Nature Structural Biology
10, 334 - 341 (2003)
Published online: 21 April 2003; | doi:10.1038/nsb922
Dynamics of herpes simplex virus capsid maturation visualized by time-lapse cryo-electron microscopyJ. Bernard Heymann, Naiqian Cheng, William W. Newcomb, Benes L. Trus, Jay C. Brown
& Alasdair C. StevenSupplementary Video 1 (mov 3435KB)
During the maturation process, the herpes simplex virus 1 precursor particle (procapsid) progresses through a succession of states from the naïve procapsid (state 1) to the fully mature capsid (state 17). The states were captured by cryo-EM and image reconstruction and are displayed here as time-lapse videos. The number (upper left) refers to the state. The particle is a T = 16 icosahedron, 1,250 Å in diameter. Video 1 shows the changes in the outer surface. Supplementary Video 2 (mov 4032KB)
Video 2 shows the changes in the inner surface of the capsid viewed along a two-fold axis of symmetry. Supplementary Video 3 (mov 3834KB)
Video 3 is a close-up view of a portion of the inner surface. Supplementary Video 4 (mov 3674KB)
Video 4 is a close-up view of a portion the outer surface, centered on a peripentonal hexon (P-hexon). To its left is a penton, and to its right, an E-hexon (edge hexon). Supplementary Video 5 (mov 3082KB)
Video 5 shows a cutaway view through part of the surface shell, which is 150 Å thick. It sections through hexons and pentons of the major capsid protein and through the triplex complexes located at local three-fold symmetry axes. The inner scaffolding shell is not shown.
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