Review

Nature Reviews Genetics 11, 685-696 (October 2010) | doi:10.1038/nrg2841

Article series: Applications of next-generation sequencing

Advances in understanding cancer genomes through second-generation sequencing

Matthew Meyerson1,2, Stacey Gabriel1,2 & Gad Getz1,2  About the authors

Top

Cancers are caused by the accumulation of genomic alterations. Therefore, analyses of cancer genome sequences and structures provide insights for understanding cancer biology, diagnosis and therapy. The application of second-generation DNA sequencing technologies (also known as next-generation sequencing) — through whole-genome, whole-exome and whole-transcriptome approaches — is allowing substantial advances in cancer genomics. These methods are facilitating an increase in the efficiency and resolution of detection of each of the principal types of somatic cancer genome alterations, including nucleotide substitutions, small insertions and deletions, copy number alterations, chromosomal rearrangements and microbial infections. This Review focuses on the methodological considerations for characterizing somatic genome alterations in cancer and the future prospects for these approaches.

Author affiliations

  1. Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA.
  2. Broad Institute, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Correspondence to: Matthew Meyerson1,2 Email: matthew_meyerson@dfci.harvard.edu

Published online 17 September 2010

MORE ARTICLES LIKE THIS

These links to content published by NPG are automatically generated.

REVIEWS

Sequencing technologies ??? the next generation

Nature Reviews Genetics Review (01 Jan 2010)

See all 9 matches for Reviews

NEWS AND VIEWS

Targeted sequencing with microfluidics

Nature Biotechnology News and Views (01 Nov 2009)