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Standardizing animal atherosclerosis studies to improve reproducibility

Nature Reviews Cardiology volume 14, pages 574–575 (2017)Cite this article

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Contradictory results in animal atherosclerosis studies might partly explain limited translational efficacy, and also undermine the confidence of funding agencies, politicians, and the public in scientists and their research. A new guideline paper provides recommendations aimed at standardization of animal atherosclerosis studies to improve the reproducibility of this research.

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Figure 1: Advanced atherosclerotic lesion in a hypercholesterolaemic mouse.

References

  1. Daugherty, A. et al. Recommendation on design, execution, and reporting of animal atherosclerosis studies: a scientific statement from the American Heart Association. Circ. Res. http://dx.doi.org/10.1161/RES.0000000000000169 (2017).

  2. Franck, G. et al. Flow perturbation mediates neutrophil recruitment and potentiates endothelial injury via TLR2 in mice: implications for superficial erosion. Circ. Res. 121, 31–42 (2017).

    Article  CAS  Google Scholar 

  3. Hartwig, H. et al. Atherosclerotic plaque destabilization in mice: a comparative study. PLoS ONE 10, e0141019 (2015).

    Article  Google Scholar 

  4. Silvestre-Roig, C. et al. Atherosclerotic plaque destabilization: mechanisms, models, and therapeutic strategies. Circ. Res. 114, 214–226 (2014).

    Article  CAS  Google Scholar 

  5. Warnatsch, A., Ioannou, M., Wang, Q. & Papayannopoulos, V. Inflammation. Neutrophil extracellular traps license macrophages for cytokine production in atherosclerosis. Science 349, 316–320 (2015).

    Article  CAS  Google Scholar 

  6. Soehnlein, O., Ortega-Gómez, A., Döring, Y. & Weber, C. Neutrophil-macrophage interplay in atherosclerosis: protease-mediated cytokine processing versus NET release. Thromb. Haemost. 114, 866–867 (2015).

    Article  Google Scholar 

  7. Heidt, T. et al. Chronic variable stress activates hematopoietic stem cells. Nat. Med. 20, 754–758 (2014).

    Article  CAS  Google Scholar 

  8. Stewart, S., Keates, A. K., Redfern, A. & McMurray, J. J. V. Seasonal variations in cardiovascular disease. Nat. Rev. Cardiol. http://dx.doi.org/10.1038/nrcardio.2017.76 (2017).

  9. Steffens, S. et al. Circadian control of inflammatory processes in atherosclerosis and its complications. Arterioscler. Thromb. Vasc. Biol. 37, 1022–1028 (2017).

    Article  CAS  Google Scholar 

  10. Llovera, G. et al. Results of a preclinical randomized controlled multicenter trial (pRCT): anti-CD49d treatment for acute brain ischemia. Sci. Transl. Med. 7, 299ra121 (2015).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Oliver Soehnlein and Carlos Silvestre-Roig are at the Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University Munich, Pettenkoferstrasse 9, 80336 Munich, Germany; and the German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany.,

    Oliver Soehnlein & Carlos Silvestre-Roig

  2. and the German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany.,

    Oliver Soehnlein & Carlos Silvestre-Roig

  3. Oliver Soehnlein is also at the Department of Physiology and Pharmacology, Karolinska Institutet, von Eulers Väg 8, 17177 Stockholm, Sweden.,

    Oliver Soehnlein

Authors
  1. Oliver Soehnlein
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  2. Carlos Silvestre-Roig
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Corresponding author

Correspondence to Oliver Soehnlein.

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The authors declare no competing financial interests.

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Soehnlein, O., Silvestre-Roig, C. Standardizing animal atherosclerosis studies to improve reproducibility. Nat Rev Cardiol 14, 574–575 (2017). https://doi.org/10.1038/nrcardio.2017.132

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