Volume 9 Issue 3, March 2014

The axolotl (Ambystoma mexicanum) is a very useful model organism for studying limb and spinal cord regeneration. The protocol by Khattak et al. (doi: 10.1038/nprot.2014.040) describes optimized axolotl husbandry, breeding, metamorphosis, transgenesis and tamoxifen-induced, Cre-mediated recombination.

Protocol

Determining cell division symmetry through the dissection of dividing cells using single-cell expression analysis

Lukasz Jasnos
Tomoyuki Sawado
Protocol 06 Feb 2014
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Quantifying DNA double-strand breaks induced by site-specific endonucleases in living cells by ligation-mediated purification

Catherine Chailleux
François Aymard
Didier Trouche
Protocol 06 Feb 2014
Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination

Shahryar Khattak
Prayag Murawala
Elly M Tanaka
Protocol 06 Feb 2014
Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone–labeled disaccharides with LC-fluorescence and LC-MS detection

Nicola Volpi
Fabio Galeotti
Robert J Linhardt
Protocol 06 Feb 2014
Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains

Anne Czechanski
Candice Byers
Laura G Reinholdt
Protocol 06 Feb 2014
Live imaging and quantitative analysis of gastrulation in mouse embryos using light-sheet microscopy and 3D tracking tools

Takehiko Ichikawa
Kenichi Nakazato
Shigenori Nonaka
Protocol 13 Feb 2014
Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh

Aurélien Rizk
Grégory Paul
Ivo F Sbalzarini
Protocol 13 Feb 2014
Quadratic isothermal amplification for the detection of microRNA

Ruixue Duan
Xiaolei Zuo
Fan Xia
Protocol 13 Feb 2014
Capturing and cultivating single bacterial cells in gel microdroplets to obtain near-complete genomes

Armand E K Dichosa
Ashlynn R Daughton
Cliff S Han
Protocol 13 Feb 2014
Control of gene expression using a red- and far-red light–responsive bi-stable toggle switch

Konrad Müller
Matias D Zurbriggen
Wilfried Weber
Protocol 20 Feb 2014
Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths

Luc Beaufort
Nicolas Barbarin
Yves Gally
Protocol 20 Feb 2014
A guide to small-molecule structure assignment through computation of (¹H and ¹³C) NMR chemical shifts

Patrick H Willoughby
Matthew J Jansma
Thomas R Hoye
Protocol 20 Feb 2014
Preparation of the planarian Schmidtea mediterranea for high-resolution histology and transmission electron microscopy

John L Brubacher
Ana P Vieira
Phillip A Newmark
Protocol 20 Feb 2014
A general protocol for the generation of Nanobodies for structural biology

Els Pardon
Toon Laeremans
Jan Steyaert
Protocol 27 Feb 2014
Microfluidic, marker-free isolation of circulating tumor cells from blood samples

Nezihi Murat Karabacak
Philipp S Spuhler
Mehmet Toner
Protocol 27 Feb 2014
Mapping the miRNA interactome by cross-linking ligation and sequencing of hybrids (CLASH)

Aleksandra Helwak
David Tollervey
Protocol 27 Feb 2014
Chemical ablation of tumor-initiating human pluripotent stem cells

Uri Ben-David
Nissim Benvenisty
Protocol 27 Feb 2014

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Corrigendum

Correction: Corrigendum: Genome-wide chromatin analysis in mature mouse and human spermatozoa

Mizue Hisano
Serap Erkek
Antoine H F M Peters
Corrigendum 11 Mar 2014

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Volume 9 Issue 3, March 2014

Protocol

Determining cell division symmetry through the dissection of dividing cells using single-cell expression analysis

Quantifying DNA double-strand breaks induced by site-specific endonucleases in living cells by ligation-mediated purification

Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination

Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone–labeled disaccharides with LC-fluorescence and LC-MS detection

Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains

Live imaging and quantitative analysis of gastrulation in mouse embryos using light-sheet microscopy and 3D tracking tools

Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh

Quadratic isothermal amplification for the detection of microRNA

Capturing and cultivating single bacterial cells in gel microdroplets to obtain near-complete genomes

Control of gene expression using a red- and far-red light–responsive bi-stable toggle switch

Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths

A guide to small-molecule structure assignment through computation of (¹H and ¹³C) NMR chemical shifts

Preparation of the planarian Schmidtea mediterranea for high-resolution histology and transmission electron microscopy

A general protocol for the generation of Nanobodies for structural biology

Microfluidic, marker-free isolation of circulating tumor cells from blood samples

Mapping the miRNA interactome by cross-linking ligation and sequencing of hybrids (CLASH)

Chemical ablation of tumor-initiating human pluripotent stem cells

Corrigendum

Correction: Corrigendum: Genome-wide chromatin analysis in mature mouse and human spermatozoa

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