Volume 9 Issue 1, January 2014

On the cover is an image of a fly using its rear leg to clean itself during a grooming reflex. Fruit flies have many innate behaviors that allow neuroscientists to investigate how the hard-wired neural circuits underlying these behaviors self-assemble. Image from the protocol by Kays et al. doi: 10.1038/nprot.2013.157. Cover design by Jamel Wooten.

Protocol

Structural and functional analysis of single neurons to correlate synaptic connectivity with grooming behavior

Ibrahim Kays
Vedrana Cvetkovska
Brian E Chen
Protocol 05 Dec 2013
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The synthesis of tetra-modified RNA for the multidimensional control of gene expression via light-activated RNA interference

Ashish Kala
Piyush K Jain
Simon H Friedman
Protocol 05 Dec 2013
Preparation of carboxylate derivatives of terpyridine via the furan pathway

Jérôme Husson
Jérémy Dehaudt
Laurent Guyard
Protocol 05 Dec 2013
Synthesis and use of QCy7-derived modular probes for the detection and imaging of biologically relevant analytes

Orit Redy-Keisar
Einat Kisin-Finfer
Doron Shabat
Protocol 05 Dec 2013
Proteome-wide enrichment of proteins modified by lysine methylation

Scott M Carlson
Kaitlyn E Moore
Or Gozani
Protocol 05 Dec 2013
Identification and quantification of aminophospholipid molecular species on the surface of apoptotic and activated cells

Christopher P Thomas
Stephen R Clark
Valerie B O'Donnell
Protocol 12 Dec 2013
Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications

Jia Guo
Matthew J Gaffrey
Wei-Jun Qian
Protocol 12 Dec 2013
Live-cell imaging of receptors around postsynaptic membranes

Hiromitsu Tanaka
Shumpei Fujii
Tomoo Hirano
Protocol 12 Dec 2013
Discovery of protein interactions using parallel analysis of translated ORFs (PLATO)

H Benjamin Larman
Anthony C Liang
Jian Zhu
Protocol 12 Dec 2013
Copper-catalyzed asymmetric conjugate addition of alkylzirconium reagents to cyclic enones to form quaternary centers

Philippe M C Roth
Mireia Sidera
Stephen P Fletcher
Protocol 12 Dec 2013
A plate-based electrochromic approach for the high-throughput detection of electrochemically active bacteria

Shi-Jie Yuan
Wen-Wei Li
Han-Qing Yu
Protocol 19 Dec 2013
Lysine-specific chemical cross-linking of protein complexes and identification of cross-linking sites using LC-MS/MS and the xQuest/xProphet software pipeline

Alexander Leitner
Thomas Walzthoeni
Ruedi Aebersold
Protocol 19 Dec 2013
Using 5-deoxy-5-[¹⁸F]fluororibose to glycosylate peptides for positron emission tomography

Xiang-Guo Li
Kerttuli Helariutta
Anu J Airaksinen
Protocol 19 Dec 2013
Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria

Rita L Strack
Wenjiao Song
Samie R Jaffrey
Protocol 19 Dec 2013
Validation of metal-binding sites in macromolecular structures with the CheckMyMetal web server

Heping Zheng
Mahendra D Chordia
Wladek Minor
Protocol 19 Dec 2013
Full-length RNA-seq from single cells using Smart-seq2

Simone Picelli
Omid R Faridani
Rickard Sandberg
Protocol 02 Jan 2014
Determining the macropinocytic index of cells through a quantitative image-based assay

Cosimo Commisso
Rory J Flinn
Dafna Bar-Sagi
Protocol 02 Jan 2014
Extensible visualization and analysis for multidimensional images using Vaa3D

Hanchuan Peng
Alessandro Bria
Fuhui Long
Protocol 02 Jan 2014
Intravascular staining for discrimination of vascular and tissue leukocytes

Kristin G Anderson
Katrin Mayer-Barber
David Masopust
Protocol 02 Jan 2014
Synchronized fission yeast meiosis using an ATP analog–sensitive Pat1 protein kinase

Lubos Cipak
Silvia Polakova
Juraj Gregan
Protocol 02 Jan 2014

Volume 9 Issue 1, January 2014

Protocol

Structural and functional analysis of single neurons to correlate synaptic connectivity with grooming behavior

The synthesis of tetra-modified RNA for the multidimensional control of gene expression via light-activated RNA interference

Preparation of carboxylate derivatives of terpyridine via the furan pathway

Synthesis and use of QCy7-derived modular probes for the detection and imaging of biologically relevant analytes

Proteome-wide enrichment of proteins modified by lysine methylation

Identification and quantification of aminophospholipid molecular species on the surface of apoptotic and activated cells

Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications

Live-cell imaging of receptors around postsynaptic membranes

Discovery of protein interactions using parallel analysis of translated ORFs (PLATO)

Copper-catalyzed asymmetric conjugate addition of alkylzirconium reagents to cyclic enones to form quaternary centers

A plate-based electrochromic approach for the high-throughput detection of electrochemically active bacteria

Lysine-specific chemical cross-linking of protein complexes and identification of cross-linking sites using LC-MS/MS and the xQuest/xProphet software pipeline

Using 5-deoxy-5-[¹⁸F]fluororibose to glycosylate peptides for positron emission tomography

Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria

Validation of metal-binding sites in macromolecular structures with the CheckMyMetal web server

Full-length RNA-seq from single cells using Smart-seq2

Determining the macropinocytic index of cells through a quantitative image-based assay

Extensible visualization and analysis for multidimensional images using Vaa3D

Intravascular staining for discrimination of vascular and tissue leukocytes

Synchronized fission yeast meiosis using an ATP analog–sensitive Pat1 protein kinase

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