Volume 17 Issue 1, January 2022

This Perspective discusses the use of CRISPR–Cas9-mediated gene conversion systems in laboratory and wild rodents. Although technical hurdles must be overcome before wild-release strategies are feasible, modest improvements could increase the efficiency and reduce the cost of mouse genetics in the laboratory.

A protocol is described for generating human brain assembloids and performing viral labeling and retrograde tracing, 3D live imaging of axon projection and optogenetics with calcium imaging and electrophysiological recordings to model neural circuits.

This protocol describes an experimental and computational approach for mapping higher-order DNA interactions that relies on tagging cross-linked fragmented chromatin through an iterative split-and-pool barcoding process.

This protocol describes a method for blood–brain barrier opening (BBBO) under the guidance of interventional MRI in mice. The method, which considerably reduces BBBO variability, can be used to improve delivery of therapeutics to the brain.

This protocol describes an in vitro model of the human blood–brain barrier, self-assembled within microfluidic devices from stem-cell-derived or primary brain endothelial cells, and primary brain pericytes and astrocytes.

Peak table processing is essential for metabolomics, but finding the best workflow is challenging. This protocol describes NOREVA, an out-of-the-box software tool that can process and evaluate thousands of workflows in a single experiment.

Many biological complexes are flexible or heterogeneous. Integrative modeling using Assembline enables structure determination of these macromolecular complexes by combining data from multiple experimental sources, including electron microscopy maps.