Volume 8 Issue 1, January 2013

SHINERS has broken the long-standing surface analysis versatility limitation of SERS. SHINs also offer superior stability and better reproducibility compared with the bare Au nanoparticles shown here, which aggregate in aqueous pyridine solution after several minutes. Image taken from the protocol by Li et al. 10.1038/nprot.2012.141. Cover design by Jamel Wooten.

Protocol

Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system

Minjoung Kyoung
Yunxiang Zhang
Axel T Brunger
Protocol 06 Dec 2012
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Global metabolic profiling of animal and human tissues via UPLC-MS

Elizabeth J Want
Perrine Masson
Jeremy K Nicholson
Protocol 06 Dec 2012
Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells

Enrico Lugli
Luca Gattinoni
Mario Roederer
Protocol 06 Dec 2012
Isolating lipid droplets from multiple species

Yunfeng Ding
Shuyan Zhang
Pingsheng Liu
Protocol 06 Dec 2012
Surface analysis using shell-isolated nanoparticle-enhanced Raman spectroscopy

Jian Feng Li
Xiang Dong Tian
Zhong Qun Tian
Protocol 13 Dec 2012
Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy

Nick Goeden
Alexandre Bonnin
Protocol 13 Dec 2012
A method to resolve the composition of heterogeneous affinity-purified protein complexes assembled around a common protein by chemical cross-linking, gel electrophoresis and mass spectrometry

Elena L Rudashevskaya
Roberto Sacco
Keiryn L Bennett
Protocol 13 Dec 2012
Multiscale analysis of genome-wide replication timing profiles using a wavelet-based signal-processing algorithm

Benjamin Audit
Antoine Baker
Alain Arneodo
Protocol 13 Dec 2012
Adapting human pluripotent stem cells to high-throughput and high-content screening

Sabrina C Desbordes
Lorenz Studer
Protocol 20 Dec 2012
Site-directed spin-labeling of nucleotides and the use of in-cell EPR to determine long-range distances in a biologically relevant environment

Mykhailo Azarkh
Vijay Singh
Malte Drescher
Protocol 20 Dec 2012
Thermodynamic analysis of protein-ligand binding interactions in complex biological mixtures using the stability of proteins from rates of oxidation

Erin C Strickland
M Ariel Geer
Michael C Fitzgerald
Protocol 20 Dec 2012
Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

Xiaojun Lian
Jianhua Zhang
Sean P Palecek
Protocol 20 Dec 2012
Transcriptome-wide mapping of N⁶-methyladenosine by m⁶A-seq based on immunocapturing and massively parallel sequencing

Dan Dominissini
Sharon Moshitch-Moshkovitz
Gideon Rechavi

Collection:

The Epitranscriptome
Protocol 03 Jan 2013
Cytometric fingerprinting for analyzing microbial intracommunity structure variation and identifying subcommunity function

Christin Koch
Susanne Günther
Susann Müller
Protocol 03 Jan 2013
Directed differentiation of human pluripotent cells to neural crest stem cells

Laura Menendez
Michael J Kulik
Stephen Dalton
Protocol 03 Jan 2013
Analyzing membrane remodeling and fission using supported bilayers with excess membrane reservoir

Sylvia Neumann
Thomas J Pucadyil
Sandra L Schmid
Protocol 03 Jan 2013

Volume 8 Issue 1, January 2013

Protocol

Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system

Global metabolic profiling of animal and human tissues via UPLC-MS

Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells

Isolating lipid droplets from multiple species

Surface analysis using shell-isolated nanoparticle-enhanced Raman spectroscopy

Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy

A method to resolve the composition of heterogeneous affinity-purified protein complexes assembled around a common protein by chemical cross-linking, gel electrophoresis and mass spectrometry

Multiscale analysis of genome-wide replication timing profiles using a wavelet-based signal-processing algorithm

Adapting human pluripotent stem cells to high-throughput and high-content screening

Site-directed spin-labeling of nucleotides and the use of in-cell EPR to determine long-range distances in a biologically relevant environment

Thermodynamic analysis of protein-ligand binding interactions in complex biological mixtures using the stability of proteins from rates of oxidation

Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

Transcriptome-wide mapping of N⁶-methyladenosine by m⁶A-seq based on immunocapturing and massively parallel sequencing

Cytometric fingerprinting for analyzing microbial intracommunity structure variation and identifying subcommunity function

Directed differentiation of human pluripotent cells to neural crest stem cells

Analyzing membrane remodeling and fission using supported bilayers with excess membrane reservoir

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