Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis - pp1565 - 1573

Kip M Connor, Nathan M Krah, Roberta J Dennison, Christopher M Aderman, Jing Chen, Karen I Guerin, Przemyslaw Sapieha, Andreas Stahl, Keirnan L Willett & Lois E H Smith

Published online: 08 October 2009 | doi:10.1038/nprot.2009.187

Abstract - Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis | Full Text - Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis | PDF (721 KB) - Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis | Supplementary information

Subject Categories: Model organisms | Pharmacology and toxicology

Housing conditions and stimulus females: a robust social discrimination task for studying male rodent social recognition - pp1574 - 1581

Abbe H Macbeth, Jennifer Stepp Edds & W Scott Young III

Published online: 08 October 2009 | doi:10.1038/nprot.2009.141

Abstract - Housing conditions and stimulus females: a robust social discrimination task for studying male rodent social recognition | Full Text - Housing conditions and stimulus females: a robust social discrimination task for studying male rodent social recognition | PDF (428 KB) - Housing conditions and stimulus females: a robust social discrimination task for studying male rodent social recognition | Supplementary information

Subject Categories: Model organisms | Neuroscience

Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells - pp1582 - 1590

Mariusz R Wieckowski, Carlotta Giorgi, Magdalena Lebiedzinska, Jerzy Duszynski & Paolo Pinton

Published online: 08 October 2009 | doi:10.1038/nprot.2009.151

Abstract - Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells | Full Text - Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells | PDF (588 KB) - Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells

Subject Categories: Cell and developmental biology | Cell and tissue culture | Isolation, purification and separation | Model organisms

The morphological and molecular features of the epithelial-to-mesenchymal transition - pp1591 - 1613

Gema Moreno-Bueno, Héctor Peinado, Patricia Molina, David Olmeda, Eva Cubillo, Vanesa Santos, José Palacios, Francisco Portillo & Amparo Cano

Published online: 15 October 2009 | doi:10.1038/nprot.2009.152

Abstract - The morphological and molecular features of the epithelial-to-mesenchymal transition | Full Text - The morphological and molecular features of the epithelial-to-mesenchymal transition | PDF (2,142 KB) - The morphological and molecular features of the epithelial-to-mesenchymal transition | Supplementary information

Subject Categories: Cell and developmental biology | Cell and tissue culture

Differentiation of human oligodendrocytes from pluripotent stem cells - pp1614 - 1622

Bao-Yang Hu, Zhong-Wei Du & Su-Chun Zhang

Published online: 15 October 2009 | doi:10.1038/nprot.2009.186

Abstract - Differentiation of human oligodendrocytes from pluripotent stem cells | Full Text - Differentiation of human oligodendrocytes from pluripotent stem cells | PDF (1,007 KB) - Differentiation of human oligodendrocytes from pluripotent stem cells

Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors - pp1623 - 1631

Kazuhiro Aoki & Michiyuki Matsuda

Published online: 15 October 2009 | doi:10.1038/nprot.2009.175

Abstract - Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors | Full Text - Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors | PDF (758 KB) - Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors | Supplementary information

Subject Categories: Biochemistry and protein analysis | Imaging

Investigation of mRNA quadruplex formation in Escherichia coli - pp1632 - 1640

Markus Wieland & Jörg S Hartig

Published online: 22 October 2009 | doi:10.1038/nprot.2009.111

Abstract - Investigation of mRNA quadruplex formation in : Escherichia coli: | Full Text - Investigation of mRNA quadruplex formation in Escherichia coli | PDF (549 KB) - Investigation of mRNA quadruplex formation in Escherichia coli

Subject Categories: Genetic modification | Nucleic acid based molecular biology | Spectroscopy and structural analysis

Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopy - pp1641 - 1648

Patrik Lundström, Pramodh Vallurupalli, D Flemming Hansen & Lewis E Kay

Published online: 22 October 2009 | doi:10.1038/nprot.2009.118

Abstract - Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopy | Full Text - Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopy | PDF (338 KB) - Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopy

Subject Categories: Biochemistry and protein analysis | Spectroscopy and structural analysis

A protocol for islet isolation from mouse pancreas - pp1649 - 1652

Dong-Sheng Li, Ya-Hong Yuan, Han-Jun Tu, Qing-Le Liang & Long-Jun Dai

Published online: 22 October 2009 | doi:10.1038/nprot.2009.150

Abstract - A protocol for islet isolation from mouse pancreas | Full Text - A protocol for islet isolation from mouse pancreas | PDF (304 KB) - A protocol for islet isolation from mouse pancreas

Subject Categories: Cell and tissue culture | Isolation, purification and separation | Model organisms

Induction of asymmetrical cell division to analyze spindle-dependent organelle partitioning using correlative microscopy techniques - pp1653 - 1662

Jen-Hsuan Wei & Joachim Seemann

Published online: 22 October 2009 | doi:10.1038/nprot.2009.160

Abstract - Induction of asymmetrical cell division to analyze spindle-dependent organelle partitioning using correlative microscopy techniques | Full Text - Induction of asymmetrical cell division to analyze spindle-dependent organelle partitioning using correlative microscopy techniques | PDF (2,531 KB) - Induction of asymmetrical cell division to analyze spindle-dependent organelle partitioning using correlative microscopy techniques | Supplementary information

Subject Categories: Biochemistry and protein analysis | Cell and developmental biology | Imaging

A reporter gene imaging system for monitoring microRNA biogenesis - pp1663 - 1669

Hae Young Ko, Do Won Hwang, Dong Soo Lee & Soonhag Kim

Published online: 29 October 2009 | doi:10.1038/nprot.2009.119

Abstract - A reporter gene imaging system for monitoring microRNA biogenesis | Full Text - A reporter gene imaging system for monitoring microRNA biogenesis | PDF (353 KB) - A reporter gene imaging system for monitoring microRNA biogenesis

Subject Categories: Genetic modification | Imaging

Generation of orthotopic and heterotopic human pancreatic cancer xenografts in immunodeficient mice - pp1670 - 1680

Michael P Kim, Douglas B Evans, Huamin Wang, James L Abbruzzese, Jason B Fleming & Gary E Gallick

Published online: 29 October 2009 | doi:10.1038/nprot.2009.171

Abstract - Generation of orthotopic and heterotopic human pancreatic cancer xenografts in immunodeficient mice | Full Text - Generation of orthotopic and heterotopic human pancreatic cancer xenografts in immunodeficient mice | PDF (1,115 KB) - Generation of orthotopic and heterotopic human pancreatic cancer xenografts in immunodeficient mice

Subject Category: Model organisms

Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures - pp1681 - 1698

Jianping Fu, Pan Mao & Jongyoon Han

Published online: 29 October 2009 | doi:10.1038/nprot.2009.176

Abstract - Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures | Full Text - Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures | PDF (1,261 KB) - Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures | Supplementary information

Subject Categories: Isolation, purification and separation | Nanotechnology

Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies - pp1699 - 1707

Pablo A Manavella & Raquel L Chan

Published online: 29 October 2009 | doi:10.1038/nprot.2009.178

Abstract - Transient transformation of sunflower leaf discs via an : Agrobacterium: -mediated method: applications for gene expression and silencing studies | Full Text - Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies | PDF (712 KB) - Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies

Subject Categories: Cell and tissue culture | Plant biology | Genetic analysis