Volume 3 Issue 5, May 2008

Phase contrast images of freshly prepared and cultured cell fractions from epidermis and dermis of newborn mice. This protocol by Ulrike Lichti, Joanna Anders and Stuart H. Yuspa details the isolation and culture of primary keratinocytes from newborn and adult mice epidermis in a low-calcium medium, for in vitro analysis and grafting to immunodeficient mice. Image is from the protocol by Lichti et al. Cover design by Jessica Iannuzzi.