The application of the 5-choice serial reaction time task for the assessment of visual attentional processes and impulse control in rats - pp759 - 767

Andrea Bari, Jeffrey W Dalley & Trevor W Robbins

Published online: 10 April 2008 | doi:10.1038/nprot.2008.41

Abstract - The application of the 5-choice serial reaction time task for the assessment of visual attentional processes and impulse control in rats | Full Text - The application of the 5-choice serial reaction time task for the assessment of visual attentional processes and impulse control in rats | PDF (278 KB) - The application of the 5-choice serial reaction time task for the assessment of visual attentional processes and impulse control in rats | Supplementary information

A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies - pp768 - 776

Elizabeth S Ng, Richard Davis, Edouard G Stanley & Andrew G Elefanty

Published online: 10 April 2008 | doi:10.1038/nprot.2008.42

Abstract - A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies | Full Text - A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies | PDF (587 KB) - A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies

See also: Protocol by Lerou et al.

Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging - pp777 - 783

Evan W Miller, Qiwen He & Christopher J Chang

Published online: 10 April 2008 | doi:10.1038/nprot.2008.43

Abstract - Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging | Full Text - Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging | PDF (240 KB) - Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae - pp784 - 798

David Drew, Simon Newstead, Yo Sonoda, Hyun Kim, Gunnar von Heijne & So Iwata

Published online: 10 April 2008 | doi:10.1038/nprot.2008.44

Abstract - GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in : Saccharomyces cerevisiae | Full Text - GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae | PDF (537 KB) - GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice - pp799 - 810

Ulrike Lichti, Joanna Anders & Stuart H Yuspa

Published online: 10 April 2008 | doi:10.1038/nprot.2008.50

Abstract - Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for : in vitro: analysis and for grafting to immunodeficient mice | Full Text - Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice | PDF (634 KB) - Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice

Dissecting RNA folding by nucleotide analog interference mapping (NAIM) - pp811 - 823

Christina Waldsich

Published online: 17 April 2008 | doi:10.1038/nprot.2008.45

Abstract - Dissecting RNA folding by nucleotide analog interference mapping (NAIM) | Full Text - Dissecting RNA folding by nucleotide analog interference mapping (NAIM) | PDF (382 KB) - Dissecting RNA folding by nucleotide analog interference mapping (NAIM) | Supplementary information

Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed - pp824 - 834

Yukoh Hiei & Toshihiko Komari

Published online: 17 April 2008 | doi:10.1038/nprot.2008.46

Abstract - Agrobacterium: -mediated transformation of rice using immature embryos or calli induced from mature seed | Full Text - Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed | PDF (521 KB) - Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed

Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells - pp835 - 839

Darren R Williams, Gun-Hee Kim, Myung-Ryul Lee & Injae Shin

Published online: 17 April 2008 | doi:10.1038/nprot.2008.47

Abstract - Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells | Full Text - Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells | PDF (271 KB) - Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells

A protocol for determination of anticardiolipin antibodies by ELISA - pp840 - 848

Silvia S Pierangeli & Eon Nigel Harris

Published online: 17 April 2008 | doi:10.1038/nprot.2008.48

Abstract - A protocol for determination of anticardiolipin antibodies by ELISA | Full Text - A protocol for determination of anticardiolipin antibodies by ELISA | PDF (910 KB) - A protocol for determination of anticardiolipin antibodies by ELISA

Array-MAPH: a methodology for the detection of locus copy-number changes in complex genomes - pp849 - 865

Ludmila Kousoulidou, Katrin Männik, Carolina Sismani, Olga ilina, Sven Parkel, Helen Puusepp, Neeme Tõnisson, Priit Palta, Maido Remm, Ants Kurg & Philippos C Patsalis

Published online: 24 April 2008 | doi:10.1038/nprot.2008.49

Abstract - Array-MAPH: a methodology for the detection of locus copy-number changes in complex genomes | Full Text - Array-MAPH: a methodology for the detection of locus copy-number changes in complex genomes | PDF (697 KB) - Array-MAPH: a methodology for the detection of locus copy-number changes in complex genomes

L-Measure: a web-accessible tool for the analysis, comparison and search of digital reconstructions of neuronal morphologies - pp866 - 876

Ruggero Scorcioni, Sridevi Polavaram & Giorgio A Ascoli

Published online: 24 April 2008 | doi:10.1038/nprot.2008.51

Abstract - L-Measure: a web-accessible tool for the analysis, comparison and search of digital reconstructions of neuronal morphologies | Full Text - L-Measure: a web-accessible tool for the analysis, comparison and search of digital reconstructions of neuronal morphologies | PDF (527 KB) - L-Measure: a web-accessible tool for the analysis, comparison and search of digital reconstructions of neuronal morphologies

Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products - pp877 - 882

Norihiro Tomita, Yasuyoshi Mori, Hidetoshi Kanda & Tsugunori Notomi

Published online: 24 April 2008 | doi:10.1038/nprot.2008.57

Abstract - Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products | Full Text - Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products | PDF (316 KB) - Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library - pp883 - 890

Luc Guerrier, Pier Giorgio Righetti & Egisto Boschetti

Published online: 24 April 2008 | doi:10.1038/nprot.2008.59

Abstract - Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library | Full Text - Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library | PDF (286 KB) - Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library

Bioluminescence imaging of hollow fibers in living animals: its application in monitoring molecular pathways - pp891 - 899

Guo-Jun Zhang, Tsing-Bau Chen, Richard Hargreaves, Cyrille Sur & David L Williams, Jr

Published online: 01 May 2008 | doi:10.1038/nprot.2008.52

Abstract - Bioluminescence imaging of hollow fibers in living animals: its application in monitoring molecular pathways | Full Text - Bioluminescence imaging of hollow fibers in living animals: its application in monitoring molecular pathways | PDF (359 KB) - Bioluminescence imaging of hollow fibers in living animals: its application in monitoring molecular pathways

Quantitative DNase footprint titration: a tool for analyzing the energetics of protein–DNA interactions - pp900 - 914

Keith D Connaghan-Jones, Amie D Moody & David L Bain

Published online: 01 May 2008 | doi:10.1038/nprot.2008.53

Abstract - Quantitative DNase footprint titration: a tool for analyzing the energetics of protein-DNA interactions | Full Text - Quantitative DNase footprint titration: a tool for analyzing the energetics of protein–DNA interactions | PDF (486 KB) - Quantitative DNase footprint titration: a tool for analyzing the energetics of protein–DNA interactions

Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites - pp915 - 922

Elijahu Berkovich, Raymond J Monnat, Jr & Michael B Kastan

Published online: 01 May 2008 | doi:10.1038/nprot.2008.54

Abstract - Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites | Full Text - Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites | PDF (189 KB) - Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites

Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos - pp923 - 933

Paul H Lerou, Akiko Yabuuchi, Hongguang Huo, Justine D Miller, Leah F Boyer, Thorsten M Schlaeger & George Q Daley

Published online: 01 May 2008 | doi:10.1038/nprot.2008.60

Abstract - Derivation and maintenance of human embryonic stem cells from poor-quality : in vitro: fertilization embryos | Full Text - Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos | PDF (655 KB) - Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos | Supplementary information

See also: Protocol by Ng et al.