Figure 2 - Counterflow centrifugal elutriation in the JE-6 elutriator rotor.


From the following article

Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation

Gaspar Banfalvi

Nature Protocols 3, 663 - 673 (2008) Published online: 27 March 2008

doi:10.1038/nprot.2008.34

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The centrifuge is run at 20 °C and constant speed (2,200 r.p.m., 683g). The pump is turned on and the system is washed first with 100 ml of 70% ethanol, then with 200 ml of physiological buffer (saline or PBS) to remove traces of alcohol. The physiological buffer is then replaced by freshly made elutriation fluid at an initial flow rate used for the introduction of cells into the chamber. Approximately 100 ml of the elutriation fluid is collected separately and discarded. The further, but still preliminary, run serves to remove bubbles from the system. Bubbles tend to be left behind in the elutriation chamber, in the sample-mixing chamber, in the tubing and inside the manometer. The Beckman loading chamber, which also serves as sample mixer, is used in the bypass position to trap bubbles and to compensate for pump pulsation. The bypass valve helps to remove bubbles from the sample-mixer chamber.

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