Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis - pp1565 - 1572

Sang-Dong Yoo, Young-Hee Cho & Jen Sheen

Published online: 21 June 2007 | doi:10.1038/nprot.2007.199

Abstract - Arabidopsis: mesophyll protoplasts: a versatile cell system for transient gene expression analysis | Full Text - Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis | PDF (241 KB) - Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

Palmitoylated proteins: purification and identification - pp1573 - 1584

Junmei Wan, Amy F Roth, Aaron O Bailey & Nicholas G Davis

Published online: 21 June 2007 | doi:10.1038/nprot.2007.225

Abstract - Palmitoylated proteins: purification and identification | Full Text - Palmitoylated proteins: purification and identification | PDF (313 KB) - Palmitoylated proteins: purification and identification

Analysis of protein glycosylation by mass spectrometry - pp1585 - 1602

Willy Morelle & Jean-Claude Michalski

Published online: 21 June 2007 | doi:10.1038/nprot.2007.227

Abstract - Analysis of protein glycosylation by mass spectrometry | Full Text - Analysis of protein glycosylation by mass spectrometry | PDF (425 KB) - Analysis of protein glycosylation by mass spectrometry

Electrophysiological evaluation of engrafted stem cell-derived neurons - pp1603 - 1613

Thoralf Opitz, Björn Scheffler, Barbara Steinfarz, Tanja Schmandt & Oliver Brüstle

Published online: 21 June 2007 | doi:10.1038/nprot.2007.230

Abstract - Electrophysiological evaluation of engrafted stem cell-derived neurons | Full Text - Electrophysiological evaluation of engrafted stem cell-derived neurons | PDF (361 KB) - Electrophysiological evaluation of engrafted stem cell-derived neurons

Agrobacterium-mediated transformation of maize - pp1614 - 1621

Yuji Ishida, Yukoh Hiei & Toshihiko Komari

Published online: 21 June 2007 | doi:10.1038/nprot.2007.241

Abstract - Agrobacterium: -mediated transformation of maize | Full Text - Agrobacterium-mediated transformation of maize | PDF (324 KB) - Agrobacterium-mediated transformation of maize

See also: Protocol by Estrada-Navarrete et al.

Robust analysis of 5'-transcript ends: a high-throughput protocol for characterization of sequence diversity of transcription start sites - pp1622 - 1632

Malali Gowda, Huameng Li & Guo-Liang Wang

Published online: 21 June 2007 | doi:10.1038/nprot.2007.242

Abstract - Robust analysis of 5[prime]-transcript ends: a high-throughput protocol for characterization of sequence diversity of transcription start sites | Full Text - Robust analysis of 5'-transcript ends: a high-throughput protocol for characterization of sequence diversity of transcription start sites | PDF (232 KB) - Robust analysis of 5'-transcript ends: a high-throughput protocol for characterization of sequence diversity of transcription start sites

Crystallization of soluble proteins in vapor diffusion for x-ray crystallography - pp1633 - 1651

Manuela Benvenuti & Stefano Mangani

Published online: 28 June 2007 | doi:10.1038/nprot.2007.198

Abstract - Crystallization of soluble proteins in vapor diffusion for x-ray crystallography | Full Text - Crystallization of soluble proteins in vapor diffusion for x-ray crystallography | PDF (385 KB) - Crystallization of soluble proteins in vapor diffusion for x-ray crystallography

Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology - pp1652 - 1660

Kai Hilpert & Robert E W Hancock

Published online: 28 June 2007 | doi:10.1038/nprot.2007.203

Abstract - Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology | Full Text - Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology | PDF (408 KB) - Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology

Construction and screening of BAC libraries made from Brachypodium genomic DNA - pp1661 - 1674

Kerrie Farrar & Iain S Donnison

Published online: 28 June 2007 | doi:10.1038/nprot.2007.204

Abstract - Construction and screening of BAC : l: ibraries made from : Brachypodium: genomic DNA | Full Text - Construction and screening of BAC libraries made from Brachypodium genomic DNA | PDF (512 KB) - Construction and screening of BAC libraries made from Brachypodium genomic DNA | Supplementary information

Systematic assessment of training-induced changes in corticospinal output to hand using frameless stereotaxic transcranial magnetic stimulation - pp1675 - 1684

Jeffrey A Kleim, Erin D Kleim & Steven C Cramer

Published online: 28 June 2007 | doi:10.1038/nprot.2007.206

Abstract - Systematic assessment of training-induced changes in corticospinal output to hand using frameless stereotaxic transcranial magnetic stimulation | Full Text - Systematic assessment of training-induced changes in corticospinal output to hand using frameless stereotaxic transcranial magnetic stimulation | PDF (419 KB) - Systematic assessment of training-induced changes in corticospinal output to hand using frameless stereotaxic transcranial magnetic stimulation

Unbiased identification of cysteine S-nitrosylation sites on proteins - pp1685 - 1691

Behrad Derakhshan, Pamela C Wille & Steven S Gross

Published online: 28 June 2007 | doi:10.1038/nprot.2007.210

Abstract - Unbiased identification of cysteine : S: -nitrosylation sites on proteins | Full Text - Unbiased identification of cysteine S-nitrosylation sites on proteins | PDF (242 KB) - Unbiased identification of cysteine S-nitrosylation sites on proteins

High-efficiency transfection of mammalian neurons via nucleofection - pp1692 - 1704

Manuel Zeitelhofer, John P Vessey, Yunli Xie, Fabian Tübing, Sabine Thomas, Michael Kiebler & Ralf Dahm

Published online: 28 June 2007 | doi:10.1038/nprot.2007.226

Abstract - High-efficiency transfection of mammalian neurons via nucleofection | Full Text - High-efficiency transfection of mammalian neurons via nucleofection | PDF (349 KB) - High-efficiency transfection of mammalian neurons via nucleofection

Imaging malaria sporozoites in the dermis of the mammalian host - pp1705 - 1712

Rogerio Amino, Sabine Thiberge, Samantha Blazquez, Patricia Baldacci, Olivier Renaud, Spencer Shorte & Robert Ménard

Published online: 05 July 2007 | doi:10.1038/nprot.2007.120

Abstract - Imaging malaria sporozoites in the dermis of the mammalian host | Full Text - Imaging malaria sporozoites in the dermis of the mammalian host | PDF (442 KB) - Imaging malaria sporozoites in the dermis of the mammalian host | Supplementary information

See also: Protocol by Thiberge et al.

Automated generation of a dihydropyrimidine compound library using microwave-assisted processing - pp1713 - 1721

Doris Dallinger & C Oliver Kappe

Published online: 05 July 2007 | doi:10.1038/nprot.2007.224

Abstract - Automated generation of a dihydropyrimidine compound library using microwave-assisted processing | Full Text - Automated generation of a dihydropyrimidine compound library using microwave-assisted processing | PDF (718 KB) - Automated generation of a dihydropyrimidine compound library using microwave-assisted processing

Quantitative analysis of chromosome conformation capture assays (3C-qPCR) - pp1722 - 1733

Hélène Hagège, Petra Klous, Caroline Braem, Erik Splinter, Job Dekker, Guy Cathala, Wouter de Laat & Thierry Forné

Published online: 05 July 2007 | doi:10.1038/nprot.2007.243

Abstract - Quantitative analysis of chromosome conformation capture assays (3C-qPCR) | Full Text - Quantitative analysis of chromosome conformation capture assays (3C-qPCR) | PDF (254 KB) - Quantitative analysis of chromosome conformation capture assays (3C-qPCR)

Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping - pp1734 - 1739

Jose Luis Royo, Manuel Hidalgo & Agustin Ruiz

Published online: 05 July 2007 | doi:10.1038/nprot.2007.244

Abstract - Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping | Full Text - Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping | PDF (263 KB) - Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping

Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivo monitoring of mercury - pp1740 - 1745

Young-Keun Yang, Sung-Kyun Ko, Injae Shin & Jinsung Tae

Published online: 05 July 2007 | doi:10.1038/nprot.2007.246

Abstract - Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivo monitoring of mercury | Full Text - Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivo monitoring of mercury | PDF (319 KB) - Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivo monitoring of mercury

Direct ester condensation catalyzed by bulky diarylammonium pentafluorobenzenesulfonates - pp1746 - 1751

Akira Sakakura, Shoko Nakagawa & Kazuaki Ishihara

Published online: 05 July 2007 | doi:10.1038/nprot.2007.254

Abstract - Direct ester condensation catalyzed by bulky diarylammonium pentafluorobenzenesulfonates | Full Text - Direct ester condensation catalyzed by bulky diarylammonium pentafluorobenzenesulfonates | PDF (283 KB) - Direct ester condensation catalyzed by bulky diarylammonium pentafluorobenzenesulfonates

Studying the biodistribution of positron emission tomography reporter probes in mice - pp1752 - 1755

Shahriar S Yaghoubi, Frank Berger & Sanjiv S Gambhir

Published online: 12 July 2007 | doi:10.1038/nprot.2007.228

Abstract - Studying the biodistribution of positron emission tomography reporter probes in mice | Full Text - Studying the biodistribution of positron emission tomography reporter probes in mice | PDF (208 KB) - Studying the biodistribution of positron emission tomography reporter probes in mice | Supplementary information

Ancient DNA extraction from bones and teeth - pp1756 - 1762

Nadin Rohland & Michael Hofreiter

Published online: 12 July 2007 | doi:10.1038/nprot.2007.247

Abstract - Ancient DNA extraction from bones and teeth | Full Text - Ancient DNA extraction from bones and teeth | PDF (201 KB) - Ancient DNA extraction from bones and teeth | Supplementary information

Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P - pp1763 - 1769

Sunil Chhatre, Nigel J Titchener-Hooker, Anthony R Newcombe & Eli Keshavarz–Moore

Published online: 12 July 2007 | doi:10.1038/nprot.2007.253

Abstract - Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P | Full Text - Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P | PDF (202 KB) - Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P

Measuring distances in proteins by saturation-recovery EPR - pp1770 - 1781

Donald J Hirsh & Gary W Brudvig

Published online: 12 July 2007 | doi:10.1038/nprot.2007.255

Abstract - Measuring distances in proteins by saturation-recovery EPR | Full Text - Measuring distances in proteins by saturation-recovery EPR | PDF (373 KB) - Measuring distances in proteins by saturation-recovery EPR | Supplementary information

Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture - pp1782 - 1788

Amy L Paguirigan & David J Beebe

Published online: 12 July 2007 | doi:10.1038/nprot.2007.256

Abstract - Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture | Full Text - Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture | PDF (501 KB) - Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture

In vitro generation of CD4+CD25+ regulatory cells from murine naive T cells - pp1789 - 1794

Massimo C Fantini, Sabine Dominitzki, Angelo Rizzo, Markus F Neurath & Christoph Becker

Published online: 12 July 2007 | doi:10.1038/nprot.2007.258

Abstract - In vitro: generation of CD4+CD25+ regulatory cells from murine naive T cells | Full Text - In vitro generation of CD4+CD25+ regulatory cells from murine naive T cells | PDF (276 KB) - In vitro generation of CD4+CD25+ regulatory cells from murine naive T cells

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate - pp1795 - 1801

Mainak Das, Kerry Wilson, Peter Molnar & James J Hickman

Published online: 19 July 2007 | doi:10.1038/nprot.2007.229

Abstract - Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate | Full Text - Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate | PDF (369 KB) - Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate

Single protein production (SPP) system in Escherichia coli - pp1802 - 1810

Motoo Suzuki, Lili Mao & Masayori Inouye

Published online: 19 July 2007 | doi:10.1038/nprot.2007.252

Abstract - Single protein production (SPP) system in : Escherichia coli | Full Text - Single protein production (SPP) system in Escherichia coli | PDF (288 KB) - Single protein production (SPP) system in Escherichia coli

In vivo imaging of malaria parasites in the murine liver - pp1811 - 1818

Sabine Thiberge, Samantha Blazquez, Patricia Baldacci, Olivier Renaud, Spencer Shorte, Robert Ménard & Rogerio Amino

Published online: 19 July 2007 | doi:10.1038/nprot.2007.257

Abstract - In vivo: imaging of malaria parasites in the murine liver | Full Text - In vivo imaging of malaria parasites in the murine liver | PDF (464 KB) - In vivo imaging of malaria parasites in the murine liver | Supplementary information

See also: Protocol by Amino et al.

Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes - pp1819 - 1824

Georgina Estrada-Navarrete, Xochitl Alvarado-Affantranger, Juan-Elías Olivares, Gabriel Guillén, Claudia Díaz-Camino, Francisco Campos, Carmen Quinto, Peter M Gresshoff & Federico Sanchez

Published online: 19 July 2007 | doi:10.1038/nprot.2007.259

Abstract - Fast, efficient and reproducible genetic transformation of : Phaseolus: spp. by : Agrobacterium rhizogenes | Full Text - Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes | PDF (261 KB) - Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes

See also: Protocol by Ishida et al.

Pyruvate aldolases in chiral carbon–carbon bond formation - pp1825 - 1830

Matthew J Walters & Eric J Toone

Published online: 19 July 2007 | doi:10.1038/nprot.2007.260

Abstract - Pyruvate aldolases in chiral carbon-carbon bond formation | Full Text - Pyruvate aldolases in chiral carbon–carbon bond formation | PDF (160 KB) - Pyruvate aldolases in chiral carbon–carbon bond formation

Fluorescence in situ hybridization on vibratome sections of plant tissues - pp1831 - 1838

Pilar Prieto, Graham Moore & Peter Shaw

Published online: 19 July 2007 | doi:10.1038/nprot.2007.265

Abstract - Fluorescence : in situ: hybridization on vibratome sections of plant tissues | Full Text - Fluorescence in situ hybridization on vibratome sections of plant tissues | PDF (335 KB) - Fluorescence in situ hybridization on vibratome sections of plant tissues