Volume 11 Issue 7, July 2016

This is a protocol to perform liver transplants in mice as a model for liver disease and transplant tolerance.

Wang et al. present a protocol for direct cloning and engineering of biosynthetic gene clusters, large operons or single genes using combined RecET and Redαβ recombineering systems present within a single E. coli host.

Methylation-assisted bisulfite sequencing (MAB-seq) enables direct genome-scale mapping of 5fC and 5caC at single-base resolution to quantify DNA demethylation. Reduced representation (RRMAB-seq) also provides increased coverage on CpG-rich regions to reduce cost.

This protocol describes the isolation and culture of primary mouse RPE cells from various mouse models to produce viable RPE cells in vitro that mimic in vivo characteristics

HOCl is a highly reactive and biologically important reactive oxygen species. Yoon and co-workers describe how to prepare a rhodamine-based probe that is specific for HOCl and is suitable for imaging experiments in Drosophila and mouse cells.

This protocol describes how to perform polar body biopsy, artificial activation of human oocytes and SNP genotyping to generate genome-wide maps of female meiotic recombination and chromosome segregation outcomes.

This protocol by Lin et al. describes the preparation of a universal electrochemical biosensing platform that uses tetrahedral DNA nanostructures. The platform allows for highly sensitive detection of nucleic acids, proteins, small molecules and whole cells.

SPADE is a density-based algorithm that can be used to organize data from heterogeneous populations of single cells into a two-dimensional tree representation. It can be applied to flow and mass cytometry data sets as well as to single-cell RNA-seq data.

An understanding of bacterial polysaccharide biochemistry requires structurally well-characterized starting materials. This protocol shows the chemical synthesis and enzymatic extension of N-acetyl-d-galactosamine undecaprenyl pyrophosphate.

Step-by-step guidance for anesthesia and analgesia administration in adult and infant marmosets for MRI-guided stereotaxic brain surgery.

This protocol assesses ribosomes purified from human cells for their ability to translate reporter mRNAs in a fractionated rabbit reticulocyte lysate assay. It may help shed light on the function of specialized ribosomes.