Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Nonlinear optics

Three-in-one microscopy

Nature Photonics volume 6, pages 78–80 (2012)Cite this article

Subjects

Using extremely broadband ultrafast near-infrared pulses, scientists have demonstrated simultaneous second-harmonic-generation, third-harmonic-generation and four-wave-mixing microscopy, enabling a range of different structures and functional groups in a biological sample to be imaged at once.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Strutural information of Caemorhabditis elegans can be extracted by simultaneous SHG, THG and FWM imaging modes at a microscope focus.

References

  1. Zipfel, W. R., Williams, R. M. & Webb, W. W. Nature Biotechnol. 21, 1369–1377 (2003).

    Article  Google Scholar 

  2. Selm, R., Krauss, G., Leitenstorfer, A. & Zumbusch, A. Appl. Phys. Lett. 99, 181124 (2011).

    Article  ADS  Google Scholar 

  3. Boyd, R. W. Nonlinear Optics 3rd edn (Academic, 2008).

    Google Scholar 

  4. Min, W., Lu, S., Rueckel, M., Holtom, G. R. & Xie, X. S. Nano Lett. 9, 2423–2426 (2009).

    Article  ADS  Google Scholar 

  5. Weiner, A. M. Rev. Sci. Instr. 71, 1929–1960 (2000).

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Brett Pearson is at the Department of Physics and Astronomy, Dickinson College, Carlisle, Pennsylvania 17013, USA,

    Brett Pearson

  2. Thomas Weinacht is at the Department of Physics and Astronomy, Stony Brook University in Stony Brook, New York 11794-3800, USA,

    Thomas Weinacht

Authors
  1. Brett Pearson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas Weinacht
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Thomas Weinacht.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pearson, B., Weinacht, T. Three-in-one microscopy. Nature Photon 6, 78–80 (2012). https://doi.org/10.1038/nphoton.2011.347

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nphoton.2011.347

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing