Abstract
A key requirement for performing three-dimensional (3D) imaging using optical microscopes is that they be capable of optical sectioning by distinguishing in-focus signal from out-of-focus background. Common techniques for fluorescence optical sectioning are confocal laser scanning microscopy and two-photon microscopy. But there is increasing interest in alternative optical sectioning techniques, particularly for applications involving high speeds, large fields of view or long-term imaging. In this Review, I examine two such techniques, based on planar illumination or structured illumination. The goal is to describe the advantages and disadvantages of these techniques.
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Acknowledgements
I acknowledge the help of W. Supatto, K. Chu, D. Lim and T. Ford for critically reading this manuscript.
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Mertz, J. Optical sectioning microscopy with planar or structured illumination. Nat Methods 8, 811–819 (2011). https://doi.org/10.1038/nmeth.1709
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DOI: https://doi.org/10.1038/nmeth.1709
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