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Article
Nature Methods - 5, 61 - 67 (2007)
Published online: 23 December 2007; | doi:10.1038/nmeth1150

Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo

Kazuo Kitamura, Benjamin Judkewitz, Masanobu Kano, Winfried Denk & Michael Häusser

Supplementary Text and Figures (pdf 504K)
Supplementary Methods, Supplementary Figures 1 and 2, and Supplementary Table 1

Supplementary Movie 1 (mov 788K)
Movie of neocortical layer 2/3 neurons visualized using shadowimaging.

Supplementary Movie 2 (mov 2M)
Same as Supplementary Movie 1, except image has been inverted.

Supplementary Movie 3 (mov 2M)
Movie of neurons in the molecular layer of cerebellar cortex visualized using shadowimaging. Note that the dendrites of individual Purkinje cells are clearly visible, as well as the cell bodies of molecular layer interneurons.

Supplementary Movie 4 (mov 964K)
Movie showing targeted patching of a Purkinje cell using the negative image. Note the appearance of the dimple just prior to GOmega seal formation.


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Nature Methods
ISSN: 1548-7091
EISSN: 1548-7105
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