Nature Methods
- 4, 927 - 929 (2007)
Published online: 21 October 2007; | doi:10.1038/nmeth1112
A monoclonal antibody for G protein–coupled receptor crystallographyPeter W Day1, 4, Søren G F Rasmussen1, 4, Charles Parnot1, Juan José Fung1, Asna Masood2, Tong Sun Kobilka1, Xiao-Jie Yao1, Hee-Jung Choi1, 3, William I Weis1, 3, Daniel K Rohrer2 & Brian K Kobilka11
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, Palo Alto, California 94305, USA. 2
Medarex, Inc., 521 Cottonwood Drive, Milpitas, California 95035, USA. 3
Department of Structural Biology, Stanford University School of Medicine, 299 Campus Drive, Stanford, Palo Alto, California 94305, USA. 4
These authors contributed equally to this work.
Correspondence should be addressed to Brian K Kobilka kobilka@stanford.edu G protein–coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human  2 adrenergic (2AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.
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