Nature Methods
- 4, 907 - 909 (2007)
Published online: 14 October 2007; | doi:10.1038/nmeth1109
Microarray-based genomic selection for high-throughput resequencingDavid T Okou1, Karyn Meltz Steinberg1, 2, Christina Middle3, David J Cutler1, Thomas J Albert3 & Michael E Zwick1, 21
Department of Human Genetics, Emory University School of Medicine, 615 Michael Street, Suite 301, Atlanta, Georgia 30322, USA. 2
Program in Population Biology, Ecology and Evolution, Emory University, 1510 Clifton Road, Atlanta, Georgia 30322, USA. 3
NimbleGen Systems, Inc., 1 Science Court, Madison, Wisconsin 53711, USA.
Correspondence should be addressed to Michael E Zwick mzwick@genetics.emory.edu We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in single-investigator laboratories.
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