Two new approaches for target profiling of RNA-binding proteins require less cell material, avoid radioactive reagents and give more accurate data in a shorter time compared to previous methods.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Castello, A. et al. Cell 149, 1393–1406 (2012).
Baltz, A.G. et al. Mol. Cell 46, 674–690 (2012).
Van Nostrand, E.L. et al. Nat. Methods 13, 508–514 (2016).
Zarnegar, B.J. et al. Nat. Methods 13, 489–492 (2016).
Chi, S.W., Zang, J.B., Mele, A. & Darnell, R.B. Nature 460, 479–486 (2009).
König, J. et al. Nat. Struct. Mol. Biol. 17, 909–915 (2010).
Hafner, M. et al. Cell 141, 129–141 (2010).
Kishore, S. et al. Nat. Methods 8, 559–564 (2011).
Ray, D. et al. Nature 499, 172–177 (2013).
Lambert, N. et al. Mol. Cell 54, 887–900 (2014).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Martin, G., Zavolan, M. Redesigning CLIP for efficiency, accuracy and speed. Nat Methods 13, 482–483 (2016). https://doi.org/10.1038/nmeth.3870
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.3870
This article is cited by
-
Capture and sequencing of NAD-capped RNA sequences with NAD captureSeq
Nature Protocols (2017)