Dénervaud, N. et al. Proc. Natl. Acad. Sci. USA 110, 15842–15847 (2013).

The Saccharomyces cerevisiae GFP fusion library is an important tool for monitoring changes in protein abundance and localization in single cells in response to a perturbation. Dénervaud et al. harnessed this library to examine the global, dynamic behavior of the yeast proteome in response to DNA replication stress induced by methyl methanesulfonate (MMS). They developed a microfluidic platform consisting of 1,152 independent microchemostats for large-scale yeast strain analysis by fluorescence imaging, using a custom software pipeline to carry out all image processing steps. They captured movies of 4,085 GFP-tagged strains (1.5 × 108 cells in total), allowing them to discern substantial protein abundance changes for 124 proteins upon MMS treatment as well as localization changes for 118 proteins. They further examined a set of 506 deletion-GFP strains to identify components regulating processing-body formation upon ultraviolet irradiation.