Review Articles

This Review discusses temporal focusing microscopy and its applications in neuroscience for imaging and optogenetic activation.

This Review highlights recent discoveries and applications of anti-CRISPR (Acr) proteins that enable the regulation of CRISPR-Cas technology.

This Review discusses the principles, advantages and limitations of Brillouin microscopy, a non-invasive tool for measuring mechanical properties of biological samples in three dimensions.

This review provides an overview of machine learning techniques in protein engineering and illustrates the underlying principles with the help of case studies.

A Review on applications of deep machine learning in image analysis that offers practical guidance for biologists.

This Review discusses the strengths and limitations of methods to probe RNA–protein interactions.

This review discusses and contrasts different acoustic-tweezer technologies and their applications in biology.

A review of cellular barcoding fundamentals and applications, including powerful approaches for lineage reconstruction, genetic screening, and the recording of cellular activity and neuroanatomy.

A review of how single-cell sequencing methods can be used to improve human stem cell and tissue engineering.

This review outlines experimental considerations, advances and challenges in microbial single-cell genome sequencing and discusses the applications and scientific questions that this approach enabled.

This Review covers the steps required to create high-quality image-based profiles from high-throughput microscopy images.

This Review introduces the fundamental considerations for building a light sheet microscope, describes the pros and cons associated with available implementations, and offers practical advice for users.

Yang and Yuste review currently available technologies for optical imaging of neural circuits, comparing them to help researchers choose optimal ones for their applications.

This Review discusses key aspects of experimental design to evaluate intergenerational and transgenerational epigenetic inheritance. It describes advantages of patrilineal and matrilineal design, and it offers advice for selecting animals for breeding and for determining the weaning scheme and group size.

In this Review, the authors compare commonly used chromosome conformation capture techniques, describing their respective strengths and weaknesses, and provide advice for the end user on which approach and analysis method to use.

This Review describes the latest methods for profiling common epitranscriptomic marks, their scale, resolution and ability to quantify. It also discusses remaining challenges.

Photoacoustic imaging (PAI) can bridge the gap between high resolution optical imaging and deep tissue imaging applications. This Review introduces PAI as well as various implementations for a range of biological applications.

This Review covers genetically encoded and exogenous contrast agents for photoacoustic imaging and offers guidance for choosing optimal probes for biological applications on the basis of photophysical properties, targeting and performance.

There is a growing appreciation that mechanical forces have important roles in many aspects of biology. This review provides a survey of methods for measuring the forces exerted by cells and discusses technical barriers to their implementation.