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Described is a randomly inserting transposon that can be swapped for gene traps, coding insertions or protein tag genes, thus expanding the toolkit for Drosophila melanogaster genome engineering.
A genetic platform allows refinement of tissue-specific expression using the upstream activating sequence–GAL4 system in Drosophila melanogaster, facilitating the segmentation of complex expression patterns and allowing GAL4 expression patterns to be repurposed.
This resource describes a human MAP kinase interactome. Yeast two hybrid-derived protein interaction maps of MAP kinase pathway members are refined using multiple criteria, tested against reference sets, and functionally validated using knockdown with siRNA.
This resource provides a comprehensive evaluation of rodent locomotor profiles after different types of lesions to the central nervous system. The data set can guide the selection of suitable lesion paradigms, locomotor tasks and readouts in future animal studies.
Paired-end sequencing of human genomic DNA reveals at least 2.8 Mb of new sequence at 720 distinct loci. Complete sequencing of 1.67 Mb at 192 loci reveals extensive copy-number variation and provides a resource for genotyping these 'missing' sequences.
High-throughput yeast two-hybrid screening is used to generate the largest C. elegans interactome resource available thus far. Using an empirical quality control framework presented in Venkatesan et al., also online, the data set is evaluated for quality and is used to estimate the total size of the worm interactome.
A collection of 33,275 human Gateway entry clones and complementary in vitro protein expression methodologies are described that allow proteome-scale production of human proteins. This 'human protein factory' was validated by expression of 13,364 human proteins and assessment of activity in a variety of assays.