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Article
Nature Immunology 6, 1228 - 1235 (2005)
Published online: 6 November 2005; | doi:10.1038/ni1269

Sphingosine 1-phosphate type 1 receptor agonism inhibits transendothelial migration of medullary T cells to lymphatic sinuses

Sindy H Wei, Hugh Rosen, Melanie P Matheu, M Germana Sanna, Sheng-Kai Wang, Euijung Jo, Chi-Huey Wong, Ian Parker & Michael D Cahalan

Supplementary Fig. 1 (pdf 664K)
Schematic diagram showing orientation of the lymph node, with major anatomical specializations, during two-photon imaging of the medulla.

Supplementary Fig. 2 (pdf 756K)
Schematic diagram illustrates the mechanism by which S1P1 agonists may regulate lymphocyte egress from the node through closing of portals in the endothelial barrier.

Supplementary Fig. 3 (pdf 144K)
Characterization of competitive S1P1 receptor antagonist W123.

Supplementary Video 1 (mov 4M)
3D reconstruction of lymphatic sinuses and T cell localization.

Supplementary Video 2 (mov 2M)
Recovery of T cell motility and transendothelial migration during washout of SEW2871.

Supplementary Video 3 (mov 2M)
Constitutive T cell motility and transendothelial migration in the absence of SEW2871.

Supplementary Video 4 (mov 2M)
SEW2871 has no effect on motility in the T cell zone.

Supplementary Video 5 (mov 1M)
Passive, unidirectional flow of T cells within the medullary sinus.

Supplementary Video 6 (mov 884K)
Example of a single T cell traversing the sinus endothelial barrier during washout of SEW2871.

Supplementary Video 7 (mov 2M)
Bi-directional trafficking of an individual T cell across the sinus wall in a control axillary lymph node.

Supplementary Video 8 (mov 3M)
T cells cross the sinus endothelial barrier through stromal 'portals'.

Supplementary Video 9 (mov 3M)
W123 reverses the actions of SEW2871 by restoring T cell motility and transendothelial migration.


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Nature Immunology
ISSN: 1529-2908
EISSN: 1529-2916
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