Abstract
Megakaryocytes are unique mammalian cells that undergo polyploidization (endomitosis) during differentiation, leading to an increase in cell size and protein production that precedes platelet production. Recent evidence demonstrates that endomitosis is a consequence of a late failure in cytokinesis associated with a contractile ring defect. Here we show that the non-muscle myosin IIB heavy chain (MYH10) is expressed in immature megakaryocytes and specifically localizes in the contractile ring. MYH10 downmodulation by short hairpin RNA increases polyploidization by inhibiting the return of 4N cells to 2N, but other regulators, such as of the G1/S transition, might regulate further polyploidization of the 4N cells. Conversely, re-expression of MYH10 in the megakaryocytes prevents polyploidization and the transition of 2N to 4N cells. During polyploidization, MYH10 expression is repressed by the major megakaryocyte transcription factor RUNX1. Thus, RUNX1-mediated silencing of MYH10 is required for the switch from mitosis to endomitosis, linking polyploidization with megakaryocyte differentiation.
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Introduction
Platelets, the blood cells involved in haemostasis, arise in mammals from the fragmentation of the cytoplasm of a giant cell called megakaryocyte (MK)1,2. The large cell size is related to polyploidization, a unique process that is an integral part of MK differentiation3. The process of polyploidization, which is denoted as endomitosis4, corresponds to repeated incomplete cell cycles marked by abortive mitosis at the late phase of cytokinesis5,6 and generates cells with ploidy of 2Nx with a modal ploidy of 16N3,4. In contrast to other cells undergoing failure of cytokinesis, MK contain a single polylobulated nucleus. At the 2N to 4N transition, cleavage furrow ingression occurs, but subsequently regresses with a backward movement between the two daughter cells5,6. A similar phenomenon is observed at higher ploidy with a less profound furrow ingression7,8. The cleavage furrow is related to the development at the equator of a contractile ring consisting of myosin II and F-actin, an actomyosin complex generating the forces necessary for furrow ingression and cell separation9. Our previous results have shown that there was a very limited accumulation of non-muscle myosin IIA (MYH9) in the contractile ring of MK undergoing mitosis or endomitosis, but the existence of furrow ingression suggested that a myosin II activity still persisted in endomitosis6.
In this study, we investigated the presence of MYH10 and showed that it is expressed in immature MK where it accumulates in the contractile ring, and that its expression is silenced during differentiation. MYH10 downregulation increases the ploidy level, and inversely, MYH10 overexpression decreases the ploidy level. Moreover, we show that the Runt related transcription factor 1 (RUNX1) negatively regulates MYH10 expression and thus links the polyploidization process with terminal MK differentiation.
Results
MYH9 does not have an important role in MK endomitosis
First, we confirmed an almost complete absence of MYH9 accumulation in the contractile ring during MK mitosis or endomitosis (Fig. 1a) opposite to primary erythroblasts undergoing mitosis (Fig. 1b). MYH9 has been considered as the only non-muscle myosin II heavy chain expressed in MK and platelets10. Therefore, we studied MK-restricted myh9 knock-out (KO) mice (myh9f/f(PF4 Cre+)) to examine the role of MYH9 in MK ploidization11,12, expecting an increase in MK ploidy. Surprisingly, no significant difference in the in-vivo MK ploidy level between myh9 f/f(PF4 Cre+) (17.11N, n=6) and control mice (myh9 f/f(PF4 Cre-); 16.53N, n=4, P=0.22, t-test, ploidy measured in MK≥8N) was seen (Fig. 1c). Blebbistatin, a small molecule inhibitor of myosin13,14, has been recently shown to increase MK ploidy15. Addition of 50 μM blebbistatin (an enantiomer mixture)16 in culture led to an increase in MK mean ploidy level that was similar between myh9 f/f(PF4 Cre+) and control mice (from 10.40N to 14.72N, P=0.01, t-test, for myh9 f/f(PF4 Cre+) mice, and from 10.00N to 13.40N, P=0.01, t-test, for control mice, n=3, ploidy measured in MK≥8N; Fig. 1d).
MYH10 is expressed in the contractile ring of MK
As blebbistatin inhibits all myosin II isoforms14, we investigated the expression of the three non-muscle isoforms MYH9, MYH10 and MYH14 during human and mouse MK differentiation. We differentiated hematopoietic progenitors (CD34+ cells for human and lineage-negative cells for mouse) in the presence of thrombopoietin (TPO), the growth factor regulating MK development2. The MKs were sorted by platelet-specific membrane glycoprotein CD41 (CD41+). MYH14 was undetectable during MK differentiation, whereas MYH10 messenger RNA (mRNA) and protein were identified by quantitative reverse transcription (RT)–PCR and western blot analyses in undifferentiated MK. During human MK differentiation, MYH10 expression markedly decreased from day 6 to day 9, approximately at a time when MK have switched from mitosis to endomitosis, to become undetectable in mature MK (day 12) and blood platelets, whereas MYH9 expression increased (Fig. 2a and b). This inverse link between MYH10 expression and differentiation was confirmed by directly sorting cord blood CD34+-derived MK on the CD41 and CD42 expression at day 10 of culture. An important decrease in MYH10 expression, and inversely, an increase in MYH9 expression were detected at the protein and mRNA level in CD41highCD42high MK in comparison with CD34+CD41− cells and immature MK (CD41lowCD42low; Fig. 2c and d). During murine MK differentiation, MYH9 expression remained stable, whereas MYH10 expression was hardly detectable as soon as 24 h after the beginning of the culture (Fig. 2e and f).
We used immunofluorescence labelling to investigate the recruitment of MYH10 to the contractile ring of mitotic and endomitotic human MK at day 6 of culture. At the 2N–4N transition, MYH10 accumulated at the midzone when the cleavage furrow started to form (Fig. 2g), and the percentage of positive cells increased with telophase progression. Only 24.5% of the cells (n=85, three independent experiments) with a cleavage furrow whose depth remained lower than 75% of cell transversal diameter accumulated MYH10. At later stage, telophase (depth of cleavage furrow >75%), 57% of the cells (n=141, three independent experiments) showed MYH10 accumulation. In multipolar endomitotic MK (4N to 8N or >8N), only 9% of the cells (n=42; three independent experiments) with a cleavage furrow lower than 75% of the cell transversal diameter showed MYH10 accumulation and the protein was not located on the whole furrow (Fig. 2h). The percentage increased to 29% at the end of telophase (cleavage furrow >75% transversal diameter, 3 independent experiments and 17 late telophase cells counted). Moreover, MYH9 was diffusively present in the cytoplasm of endomitotic MK, whereas MYH10 was specifically localized in the contractile ring (midzone and midbody; Fig. 2i and j). Only a few human MK accumulated MYH9 at the cleavage furrow at the end of telophase6. Furthermore, we found that MYH9 did not accumulate in the contractile ring of dipolar murine MK in telophase (97 telophase cells were counted, 3 independent experiments). These results suggest that MYH10 is the main myosin II isoform present in the contractile ring of MK. Its downregulation during differentiation leads to a decreased myosin II accumulation in the contractile ring.
MYH10 is involved in MK ploidy regulation
To determine if MYH10 is really implicated in MK polyploidization, human progenitors (CD34+ cells) were induced towards MK differentiation (stem cell factor (SCF)+TPO) and transduced at days 2 and 3 of culture with lentiviruses encoding green fluorescent protein (GFP) and two different short hairpin RNAs (shRNAs) targeting MYH10 (B3 and B5) or a scramble shRNA. Western blot analysis of the GFP-positive population sorted at 72 h after infection with the shMYH10-B5 showed an ~50% reduction in the MYH10 protein level, whereas the level of MYH9 protein remained unchanged (Fig. 3a). MYH10 knockdown resulted in a marked increase in MK ploidy, as attested by a shift in mean ploidy from 7.65N to 9.84N for shRNA-treated MK (n=3, P=0.015, t-test; Fig. 3b). The shMYH10-B3 was less effective in the downregulation of MYH10 (Fig. 3a), as well as in the increase of ploidy. By overexpressing a MYH10 mutated form, not identified by the shRNA, we could confirm in the COS7 cell line that shMYH10 affects the cell cycle by a specific MYH10 knockdown and not by an off-target effect (Supplementary Fig. S1). Conversely, we re-expressed MYH10 in MK at day 5 of culture, using a vector containing MYH10 in fusion with GFP by nucleofection. Quantitative RT–PCR analysis of the GFP-positive populations (MYH10–GFP and control GFP) 48 h after nucleofection showed a 20-fold increase in MYH10 mRNA level, whereas MYH9 mRNA level remained unchanged (Fig. 3c). MYH10 overexpression increased the percentage of 2N MK from 30.5 to 41.9% (n=4, P=0.009, t-test) and reduced MK mean ploidy level (from 6.5N to 5.2N, n=4, P=0.01, t-test; Fig. 3d). Altogether, these results suggest that MYH10 is the main myosin II isoform implicated in MK polyploidization.
Myosin II is activated during furrow ingression
We then investigated if this low myosin accumulation in the contractile ring was capable to generate a myosin activity. Activation of myosin II was assessed by exploring the phosphorylation status of its regulatory light chain (MLC2) with an antibody recognizing PPMLC2-, Ser19- and Thr18-phosphorylated forms of MLC2. A positive signal was easily seen at late telophase in 68.6% (n=102, three independent experiments) of dipolar mitotic/endomitotic MK with a cleavage furrow ≥75% cell transversal diameter (Fig. 4a). PPMLC2 was also detected on the cleavage furrow of polyploid endomitotic MK examined at late telophase or during cytokinesis failure (Fig. 4b), but only for a minority of them (25%, n=24, three independent experiments). This experiment indicates that there still exists some myosin II activity at the contractile ring at the end of MK telophase, even with a reduced myosin heavy chain accumulation.
MLC2 phosphorylation regulates myosin II activity without affecting the recruitment of myosin II and F-actin in the contractile ring13,17,18 and controls the actin dynamics (assembly and disassembly) necessary for completion of cytokinesis19. To study actin turnover, CD34+ cells were transfected with a GFP–β-actin construct and induced to differentiate into hematopoietic cells by a cocktail of growth factors. On the basis of the expression of the platelet-specific membrane glycoprotein CD41, GFP-positive cells were sorted into MK (CD41+GFP+) and non-MK (CD41−GFP+), and actin turnover was measured in the contractile ring at the end of cytokinesis by fluorescence recovery after photobleaching in dipolar mitosis or in endomitosis (Fig. 4c, Supplementary Movie 1). Fluorescence recovery in the contractile ring after bleaching was lower in MK than in non-MK cells (55.7±12.8% with a t1/2 of 6.3±2.5 s (n=15) versus 77.6±10.9% with a t1/2 of 8.6±3.7 s (n=16); P=0.0007, t-test). This significant decrease in the actin–GFP fluorescence recovery in the contractile ring of MK as compared with control myeloid cells suggested that a high pool of non-dynamic F-actin existed at the end of cytokinesis of dipolar mitotic/endomitotic MK (Fig. 4d). Partial inhibition of myosin II activity by blebbistatin pretreatment at low concentration (12.5 μM) increased the fraction of non-dynamic F-actin in the contractile ring of MK, as attested by a decreased fluorescence recovery (42.9±9.0%, with t1/2 of 2.94±1.35 s, n=8, P=0.01 t-test with blebbistatin; Fig. 4d). Although blebbistatin is inactivated by blue light, we think that myosin activity was not restored immediately and the time until recovery explains the greater stability of F-actin. Together, these results indicate that a myosin II activity still persists in the contractile ring of MK; however, its attenuation decreased the F-actin mobility, increasing the probability of a cytokinesis failure.
To investigate the role of residual myosin II activity on furrow ingression, blebbistatin was added at day 6 of culture when polyploidy begins in MK. Ploidy level was analysed by flow cytometry 72 h later in maturing MK identified by co-expression of two platelet-specific glycoproteins, CD41 and CD42. Blebbistatin significantly increased the mean ploidy from 6.47N to 11.27N at 25 μM (n=3, P=0.015, t-test; Fig. 5a) and from 6.28N to 12.12N at 100 μM (n=4, P=0.008, t-test; Fig. 5b). When examined by real-time video microscopy, H2B–GFP-expressing MK showed an apparently normal metaphase-to-anaphase transition in the presence of blebbistatin at 25 μM (Supplementary Movie 2 and 3) as compared with controls (Supplementary Movie 4 and 5), but the furrow ingression was almost completely inhibited (Supplementary Movie 2 and 3 compared with Supplementary Movie 4 and 5; Fig. 5c and d). These data indicated that myosin II was implicated in MK furrow ingression and that its activation had a negative role for MK polyploidization.
MYH10 is involved in the switch from mitosis to endomitosis
There are two ways to increase MK ploidy: one is to augment the number of endomitotic cycles, the other is to promote the switch from mitosis to endomitosis by preventing 4N cycling cells to return to 2N. To determine the mechanism by which MYH10 knockdown modifies ploidy level, MK cultures were infected at day 0 and day 1 with the shMYH10-B5-encoding lentivirus. The cell cycle status of GFP-positive cells was analysed by bromodeoxyuridine (BrdU) incorporation 72 h after infection (day 4 of culture; three independent experiments). MYH10 knockdown had no effect on BrdU incorporation in both 2N–4N (37% with shMYH10 and 36% with short hairpin scramble sequence (shSCR)) and >4N MK populations (6% with shMYH10 and 8% with shSCR; Fig. 6a). This result demonstrated that MYH10 knockdown had no impact in the G1/S transition for both diploid and polyploid MK. Simultaneous analysis of the ploidy level showed that MYH10 knockdown diminished the frequency of 2N MK, but increased that of ≥4N MK, suggesting that MYH10 regulates the fate of 4N cells (Fig. 6b). To directly demonstrate this mechanism, 4N MKs were sorted after Hoechst 33342 labelling at day 5 of culture and cultured for additional 72 h with and without blebbistatin (25 μM) before ploidy analysis (Fig. 6c). After blebbistatin treatment, only a small fraction of 2N MKs were detected (3.4±2.3%), the majority of the cells remained 4N, and a part of MK increased their ploidy level (Fig. 6c, 4N blebbistatin). Without blebbistatin treatment, a significant fraction of 4N cells returned to 2N, (30±6% 2N MK detected, three independent experiments, P=0.03 t-test; Fig. 6c, 4N control). Collectively, these results demonstrated that MYH10 was involved in the switch from mitosis to endomitosis. This result is in agreement with previous results that have shown that MYH10 is involved in cytokinesis and that its knockdown in cell lines leads to multinucleated polyploid cells15,20,21.
RUNX1 mediates MYH10 silencing during MK differentiation
The arrest of endomitosis is linked to MK maturation and RUNX1 has a role in this process by regulating genes involved in platelet functions, including myosin light chain 9 (MYL9/MLC2)22 and the CDK inhibitor p19INK4D (ref. 23). Small diploid MKs are present in the bone marrow of runx1 KO mice24,25 suggesting that RUNX1 could also regulate the switch from mitosis to endomitosis by targeting MYH10. Using an in silico approach with the ChipMapper online software, we identified a putative conserved RUNX1 transcription factor-binding site in the MYH10 promoter region (Fig. 7a, Supplementary Fig. S2). Chromatin immunoprecipitation (ChIP) assays demonstrated that RUNX1 could bind this site in MK (Fig. 7b, two independent experiments were performed). To test for its functional relevance, we cloned the promoter region (pMYH10_luc) with or without mutation in the RUNX1-binding site (pMYH10mut_luc) upstream of the luciferase gene (Fig. 7a). We performed gene reporter assays in the human erythroleukemia cell line. The mutation in the RUNX1-binding site led to a significant increase in luciferase activity (n=3, P≤0.05, t-test; Fig. 7c). To better understand the regulatory role of RUNX1 in MK, we then transduced human CD34+ cells induced for MK differentiation at day 1 and day 2 of culture with a lentivirus encoding an shRNA-targeting RUNX1 (shRUNX1)23. As shown in Fig. 7d, shRUNX1 treatment led to a significant decrease (30–50% depending on experiment) in the RUNX1 transcripts. Real-time PCR showed a 1.5-fold increase in MYH10 expression (Fig. 7d), which was confirmed at the protein level (Fig. 8a), whereas flow cytometric analysis showed a decrease in the ploidy level of mature MK (CD41highCD42high; n=3, P≤0.01, t-test; Fig. 8b).
To confirm these results in vivo, we used a KO mouse model in which the conditional runx1f/f mouse strain24 was crossed with Mx1-Cre mice. Efficient Runx1 deletion was achieved in hematopoietic cells depleted in lineage markers (lineage-negative (Lin−)) 5 weeks after injection of polyinosinic-polyacytidylic acid (pIpC) (Fig. 8c)24. Runx1-deficient bone marrow MK exhibited a decrease in ploidy level, as compared with control littermates (mean ploidy 12N versus 18N; n=3, P≤0.01, t-test; Fig. 8d). Bone marrow Lin− cells were cultured in the presence of TPO, and CD41+ cells were sorted at day 2.5 of culture. Blebbistatin (50 μM) induced a dramatic increase in ploidy in runx1f/f(Mx1 Cre+) MK (mean ploidy from 10.8N to 22.6N, n=3, P=0.006, t-test; Fig. 8e). A significant 1.7-fold increase of Myh10 mRNA level was detected in runx1-deficient MK (Fig. 8f). Altogether, the RUNX1/MYH10 pathway appears as an important regulator of the switch from mitosis to endomitosis.
Discussion
The mechanism of the switch from mitosis to endomitosis in MK is still a matter of controversy26,27,28,29,30 with studies suggesting a cell cycle regulation defect, whereas others argue for a defect in the expression of the central spindle proteins. Our new findings demonstrate the key contribution of MYH10 downregulation in the process of the switch from mitosis to endomitosis. Evidence was provided that endomitosis was a defect in late cytokinesis with an incomplete furrow ingression5,6,7. Here we demonstrate that immature MK express myosin IIB and that the MYH10 gene is silenced during differentiation. We also show that the two non-muscle myosin II isoforms have distinct roles in MK. By accumulating in the contractile ring, MYH10 is involved in late phases of mitosis, whereas MYH9 appears to be mainly an organizer of the MK cytoplasm and is implicated in pro-platelet formation at late stages of differentiation10,12. Sustaining this hypothesis, a limited MYH9 accumulation was observed in the contractile ring of human MK6. Furthermore, MYH9 was not detected in the cleavage furrow of murine MK and an myh9 KO did not modify the MK ploidy12. Therefore, it is likely that non-muscle myosin IIA and B have two distinct functions, both in human and murine megakaryopoiesis. These two myosin II isoforms have previously been shown to have distinct roles in cadherin junction of the epithelial cells31 or in the erythroblast enucleation32. However, MYH10 silencing does not explain the entire polyploidization process. MYH10 silencing has a central role at the 2N–4N transition in MK by inducing failure of cytokinesis as also observed in MYH10-ablated cardiac myocytes21 or COS cells15,20. However, MKs are not subsequently blocked in 4N. Thus, abnormalities in the G1/S transition check point might also be present in the 4N MK3,33,34. In addition, it remains to understand why MYH9 does not accumulate in the cleavage furrow of MK in contrast to other cell types; in fact, myosin II isoform localization may depend on distinct upstream signalling pathways as shown in epithelial cells31.
RUNX1, one of the main transcription factor involved in MK differentiation, directly regulates MYH10 expression and is responsible for its repression during MK differentiation, which may explain the presence of low ploidy MK in runx1f/f(Mx1 Cre+) mice24,25, as well as in hereditary or acquired haematological disorders associated with RUNX1 loss-of-function mutations35,36. Low-ploidy MK has been observed in KO of other transcription factors, such as GATA1 or FLI-1 (refs 37,38). As these two transcription factors may be associated with RUNX1 in large transcription complexes39,40, they may similarly act on MYH10 expression. The observation that two non-muscle myosin II isoforms are present in MK and have distinct roles opens new avenues of research; for example, the MYH9/MYH10 ratio could be used as a marker of MK differentiation and dysfunction. Complete understanding of how MYH10 expression is regulated in numerous physiological and pathological conditions remains a question for further investigations.
Methods
Mice and in vitro cultures of murine MK
The conditional runx1 KO mouse strain was developed by Dr J. D. Growney et al.24 and was a gift from Dr. D. G. Gilliland (Boston, USA) and Dr. N. A. Speck (Philadelphia, USA). Runx1f/f mice were crossed with Mx1-Cre mice; deletion of runx1 locus flanked by LoxP sites was induced by injection of the interferon-inducing agent pIpC. Five weeks after runx1 KO induction, platelet counts were performed. The runx1 KO mice exhibited thrombopenia (2–3×105 platelets per ml), contrasting with mice negative for Cre-recombinase (~1×106 platelets per ml).
The floxed myh9 strain was crossed with PF4-Cre mice to obtain mice with deletion of the MYH9 exon 1 in the MK lineage, as described previously11. Lin− cells were purified from the fetal liver (E14.5) after incubation with a mixture of rat monoclonal antibodies against lineage antigens (Gr1, TER119, CD11b, B220 and anti-CD3, 15 μg ml−1 each, Pharmingen, CA, USA) and depleted with Dynabeads coupled to a mouse antibody against rat immunoglobulin (Dynal, Norway). Lin− cells were cultured in the presence of murine (SCF, 10 ng ml−1, Peprotech, USA) and TPO (50 ng ml−1, Kirin Brewery, Tokyo, Japan) to obtain murine MK. Blebbistatin (50 μM) was added at the onset of Lin− cell culture and MK ploidy was analysed after 48 h treatment by blebbistatin. Mice were housed and analysed at the Institut Gustave Roussy Animal Facilities (Villejuif, France) according to the national and institutional guidelines.
In vitro MK culture derived from human CD34+ cells
CD34+ cells were obtained in agreement with our Institute Ethic Committee (Assistance Publique des Hôpitaux de Paris) from the bone marrow of healthy patients, leukapheresis samples and cord blood. CD34+ cells were isolated by an immunomagnetic cell sorting technique (AutoMacs; Miltenyi Biotec, Germany) and cultured in serum-free medium in the presence of recombinant human TPO (10 ng ml−1)41. Depending on the experiments, MKs were sorted according to CD41, or CD41 and CD42 expression using Influx flow cytometer (BD, Mountain View, CA)42.
Fluorescence recovery after photobleaching analysis
CD34+ cells were cultured 4 days in serum-free medium with recombinant human cytokines: either a combination of TPO (10 ng ml−1) and SCF (25 ng ml−1; Biovitrum AB, Stockholm, Sweden) or a combination of TPO, interleukin-3 (100 U ml−1, Novartis, Basle, Switzerland), SCF and fetal liver tyrosine kinase ligand (10 ng ml−1, Celldex Therapeutics Inc., Needham, USA). Cells were then transfected with the GFP–β-actin construct by the Amaxa nucleofection system (Amaxa, France). Twenty-four hours after transfection, CD41+GFP+ MKs were sorted. The CD41−GFP+ cells (mainly myeloid precursors) were obtained from cultures performed with four growth factors and were used as control. Hoechst 33342 (5 μg ml−1, Sigma, France) was added in the medium 5 min before observation under a laser-scanning microscope (LSM). In some experiments, blebbistatin, an enantiomer mixture of blebbistatin (±), (Merck, Germany) disolved in dimethyl sulfoxide (DMSO; 12.5 μM) or the same volume of diluents (DMSO) was added in the medium 30 min before observation. Photobleaching experiments were performed with a Zeiss LSM (510, Carl Zeiss, Germany) using a ×63/1.4 numerical aperture oil objective at 37 °C with 5% CO2 in air. Bleaching of the marked region was carried out at 100% laser power with 100 iterations. After photobleaching, images were acquired every second for 1 min. Fluorescence recovery was analysed with the Zeiss Image Examiner and Excel softwares.
Lentiviral constructs
The EF1α-H2BGFP fragment from the BOS-H2BGFP vector (BD Pharmingen) was excised and subcloned into an HIV-derived lentiviral vector (pRRLsin-PGK-eGFP-WPRE; Genethon, France) in place of the phosphoglycerate kinase (PGK)-enhanced GFP sequence.
shRNA cloning and lentiviral construction
shRUNX1-containing lentivirus was previously described23. Two shMYH10, and control SCR containing lentiviruses were constructed as previously described23.
Lentivirus production
Lentivirus particles were prepared by transient co-transfection of human HEK293T cells with lentiviral vector plasmids (H2B-GFP6, shRNA MYH10, shRNA RUNX1, shRNA SCR), the packaging plasmid pCMVΔR8.74 and the VSV-G protein envelope plasmid. JetPrime (Ozyme, France) was used for transfection according to the manufacturer's instructions. Lentivirus stocks containing about 109 infectious particles per ml were kept frozen at −70 °C. Viruses stocks were thawed and immediately used.
Cell transduction
CD34+ cells were cultured with TPO and SCF. Lentiviral particles were added at a concentration of 107 infectious particles per 1×105 cells for 12 h followed by a second transduction, then cells were cultured in presence of TPO23.
Electroporation
pEGFP-C3-MHCB plasmid encoding for wild-type MYH10 cDNA was kindly provided by Professor Shoshana Ravid (The Hebrew University, Israel). To test for specificity of the shMYH10 in COS7 cell line, four nucleotides localized in the region recognized by the shMYH10 were mutated in MYH10 cDNA by directed mutagenesis (3928nt gcagcT/AagT/CcttgagtcT/CcaA/G 3949nt, NM_005964.1). To study MYH10 overexpression in primary MK, the cytomegalovirus promoter was replaced by the EF1α promoter. pEGFP-EF1α-MHCB and the control (pEGFP-EF1α) were delivered into MK by nucleofection (Amaxa Inc., MD).
BrdU analysis
CD34+ cells were cultured with TPO and SCF, and transduced by the shMYH10 or shSCR at day 0 and day 1 culture. GFP+ cells sorted at day 4 of culture were incubated with 10 μM BrdU for 1 h at 37 °C. Staining was performed with the BrdU flow kit according to manufacturer's instruction (BD Pharmingen). BrdU incorporation was analysed in CD41+ cells stained with propidium iodide on a LSRII (Becton Dickinson).
Ploidy analysis
Hoechst 33342 (10 μg ml−1) was added in the medium of cultured MK for 1 h at 37 °C. Cells were stained with directly coupled monoclonal antibodies: anti-CD41 APC and anti-CD42 PE (BD Biosciences) for 30 min at 4 °C26. Ploidy was measured in the CD41+/CD42+ cell population (for chemical inhibitors test) and in the CD41+/CD42+/GFP+ population (after lentiviral transduction) by means of a LSRII flow cytometer equipped with three lasers (360, 480 and 560 nM excitation; Becton Dickinson). The mean ploidy of human MK was calculated by the following formula: (2N×the number of cells at 2N ploidy level +...+64N×the number of cells at 64N ploidy level/the total number of cells). The mean ploidy of murine MK was calculated in ≥8N MK.
Mitosis and endomitosis transition analysis
Hoechst 33342 (4 μg ml−1) was added in the medium for 1 h at 37 °C before cell sorting at day 5 of culture. The 4N sorted cells were cultured for additional 72 h with and without blebbistatin (25 μM) before ploidy analysis in the CD41+ population described above.
Western blot analysis
Cells were lysed in Laemmli 2× buffer, and the lysates were then gently sonicated on ice. Samples were boiled 5 min in loading buffer and subjected to SDS–polyacylamide gel electroporesis gel. After transfer, nitrocellulose membranes were blotted with the following antibodies: rabbit anti-MYH10, anti-MYH9 (Cell Signaling, Cat. Number 3404 and Number 3403, both diluted at 1:1000) and anti-HSC70 antibodies (Stressgen, used at 0.2 μg ml−1) followed by horseradish peroxidase-linked secondary antibodies and Immobilon Western Chemiluminescent HRP Substrate (Millipore Corporation, USA).
Immunofluorescence
Immunofluorescence was performed on CD41+ MK sorted at day 6 of human culture42 or day 2 of murine culture derived from Lin− liver fetal cells. Cells were examined under the Zeiss LSM. The following antibodies were used: rabbit anti-MYH10 and anti-MYH9 antibodies (as above, both diluted at 1:100), mouse anti-MYH9 (Sigma, used at 10 μg ml−1), rabbit anti-phospho-MLC2 Thr18Ser19 (PPMLC2; gift from Dr J. M. Staddon)43, and mouse anti-α tubulin and anti-β tubulin antibodies (Sigma, both used at 20 μg ml−1). Appropriate secondary antibodies were conjugated with Alexa 488 or Alexa 546 (Molecular Probes). TOTO-3 iodide (Molecular Probes) was applied for nucleus staining.
Live-cell imaging by confocal video microscopy
CD34+ cells were cultured 3 days in the presence of TPO and then transduced by H2B-GFP lentivirus as previously described6. After transduction, cells were cultured in the presence of TPO for 48–72 h. Cells were then seeded in glass bottom culture dish (MatTek) and were imaged under the Zeiss LSM using a ×63/1.4 numerical aperture oil objective at 37 °C with 5% CO2 in air. Serial images were obtained at 3- or 5-min interval. Image analyses were performed with the Zeiss Image Examiner software.
Quantitative real-time PCR
The mRNA isolation, reverse-transcription, and RT–PCR analyses were performed as described23. The expression levels of all genes studied were expressed relatively to housekeeping genes, PPIA for human cells and HPRT for murine cells, where the PPIA level was not comparable to that of MYH10. Primer sequences are listed in Supplementary Table S1.
ChIP and promoter activity assays
ChIP assays were performed with a ChIP assay kit (Millipore Upstate Biotechnology) using the anti-RUNX1 antibody (8 μg per sample, sc-65, Santa Cruz Biotechnology). Assays were performed using chromatin prepared from human MK as previously described23. Immunoprecipitated DNA was analysed on a PRISM® 7700 sequence detection system using SYBR® green (Applied biosystems) in duplicate. Primer sequences are listed in Supplementary Table S1.
Luciferase reporter assay
The human erythroleukemia cells were transfected with the reporter plasmids, pMYH10_luc and pMYH10mut_luc, and co-transfected with TK-Renilla reporter (Promega, France) for normalization of transfection efficiency. Transfected cells were harvested at 48 h post-transfection. A dual luciferase assay was performed according to the manufacturer′s instructions (Promega, France). The luciferase activity was measured with an AutoLumat LB953 luminometer (Berthold).
Statistics
Statistical significance was determined by Student's t-test. A P-value <0.05 was considered as statistically significant. Error bars represent s.d. of duplicate or triplicate.
Additional information
How to cite this article: Lordier, L. et al. RUNX1-induced silencing of non-muscle myosin heavy chain IIB contributes to megakaryocyte polyploidization. Nat. Commun. 3:717 doi: 10.1038/ncomms1704 (2012).
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Acknowledgements
We thank Dr. D. G. Gilliland and Dr. N. A. Speck for kindly providing the Runx1 conditional knockout mouse line. We thank F. Wendling, E. Solary, O. Bernard and S. Constantinescu for critical review of the manuscript. We thank I. Godin for technical help of lineage-negative purification. We thank Kirin Breweries, Novartis, Biovitrum AB and Celldex Therapeutics Inc. for their kind gift of recombinant human TPO, IL-3, SCF and FLT3-L, respectively. This study was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM) and by grants from la Ligue Nationale Contre le Cancer (LNCC ; Equipe labellisée 2010, WV) and from the Agence Nationale de la Recherche (ANR blanc W. V., ANR Jeune chercheur H. R. and ANR Jeune chercheur Y. C.). H. R. is recipient of a research fellowship from AP-HP-INSERM (contrat d'interface 2008-2013) and W. V. from IGR-INSERM. D. B. is supported by an ANR fellowship, C. Legrand by a fellowship from the Association de la Recherche contre le Cancer (ARC) and D. J. by a predoctoral fellowship from la Société Française d'hématologie (SFH).
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W. V., H. R. and Y. C. co-directed the studies. H. R., Y. C., L. L., D. B. designed the experiments. L. L., Y. C., A. J., P. R., N. D., H. R. and J. J. P. performed the cell biology experiments. D. B., H. R. and O. B. performed the molecular biology experiments. T. M. provided transgenic mice. L. L., Y. C., C. Legrand, D. J., C. Léon, C. G. and J. P. performed mouse experiments. W. V., H. R. and Y. C. wrote the manuscript with contribution of all other authors. L. L. and D. B. contributed equally to this work. H. R. and Y. C. have contributed equally to this work.
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Supplementary information
Supplementary Figures and Supplementary Table.
Supplementary Figures S1-S2 and Supplementary Table S1 (PDF 379 kb)
Supplementary Movie 1
A dipolar human MK at the end of cytokinesis before and after photobleaching. Images are shown with rainbow illumination. They were acquired every sec and 66 images are presented. (MOV 3028 kb)
Supplementary Movie 2
Absence of furrow ingression in a blebbistatin-treated dipolar human MK during endomitosis. Time-lapse images were obtained by confocal video microscopy and serial images were obtained at 3 min interval. (MOV 3028 kb)
Supplementary Movie 3
Absence of furrow ingression in a multipolar human MK during endomitosis. Time-lapse images were obtained by confocal video microscopy and serial images were obtained at 3 min interval. (MOV 65 kb)
Supplementary Movie 4
Presence of furrow ingression in control dipolar human MK during endomitosis. Time-lapse images were obtained by confocal video microscopy and serial images were obtained at 3 min interval. (MOV 135 kb)
Supplementary Movie 5
Presence of furrow ingression in control multipolar human MK during endomitosis. Time-lapse images were obtained by confocal video microscopy and serial images were obtained at 3 min interval. (MOV 331 kb)
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Lordier, L., Bluteau, D., Jalil, A. et al. RUNX1-induced silencing of non-muscle myosin heavy chain IIB contributes to megakaryocyte polyploidization. Nat Commun 3, 717 (2012). https://doi.org/10.1038/ncomms1704
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DOI: https://doi.org/10.1038/ncomms1704
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