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The structure and mechanism of the Mycobacterium tuberculosis cyclodityrosine synthetase

Nature Chemical Biology volume 6, pages 797–799 (2010)Cite this article

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Abstract

The Mycobacterium tuberculosis enzyme Rv2275 catalyzes the formation of cyclo(L-Tyr-L-Tyr) using two molecules of Tyr-tRNATyr as substrates. The three-dimensional (3D) structure of Rv2275 was determined to 2.0-Å resolution, revealing that Rv2275 is structurally related to the class Ic aminoacyl-tRNA synthetase family of enzymes. Mutagenesis and radioactive labeling suggests a covalent intermediate in which L-tyrosine is transferred from Tyr-tRNATyr to an active site serine (Ser88) by transesterification with Glu233 serving as a critical base, catalyzing dipeptide bond formation.

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Figure 1: Cyclodityrosine synthetase reaction and 3D structure.
Figure 2: Visualization of acyl-enzyme intermediate.
Figure 3: Proposed mechanism of cyclodipeptide formation for CDPSs.

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Acknowledgements

We thank S. Del Amo and M. Callaway (Albert Einstein College of Medicine) for their help in cYY synthesis and ESI mass spectrometry, respectively. This work was supported by US National Institutes of Health Grant A133696.

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  1. Matthew W Vetting and Subray S Hegde: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA

    Matthew W Vetting, Subray S Hegde & John S Blanchard

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  1. Matthew W Vetting
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  2. Subray S Hegde
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Contributions

Funding was obtained by J.S.B. Experiments were conceived and designed by S.S.H., M.W.V. and J.S.B. S.S.H. cloned and purified Rv2275 and EcTyrRS, performed the kinetic analysis and did the radiolabeling experiments. M.W.V. determined the 3D structure of Rv2275 and cloned and purified the Rv2275 mutants. The manuscript was drafted by S.S.H. and M.W.V. and revised by all authors.

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Correspondence to John S Blanchard.

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The authors declare no competing financial interests.

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Supplementary Methods, Supplementary Tables 1–3 and Supplementary Figures 1–7 (PDF 4543 kb)

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Vetting, M., Hegde, S. & Blanchard, J. The structure and mechanism of the Mycobacterium tuberculosis cyclodityrosine synthetase. Nat Chem Biol 6, 797–799 (2010). https://doi.org/10.1038/nchembio.440

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