Standardizing data - pp1123 - 1124

doi:10.1038/ncb1008-1123

Biological research is benefiting from an explosion of data. There is an urgent need to invest in bioinformatic infrastructure and education to interpret this data and guarantee its archiving.

Full Text - Standardizing data | PDF (249 KB) - Standardizing data

ELSO into EMBO goes - p1124

doi:10.1038/ncb1008-1124

The European Life Sciences Organization is set to close shop at the end of the year by fusing with EMBO.

Full Text - ELSO into EMBO goes | PDF (87 KB) - ELSO into EMBO goes

Pores galore for the Golgi - pp1125 - 1126

John H. Caldwell & Kathryn E. Howell

doi:10.1038/ncb1008-1125

Strict control of cisternal pH in the Golgi is required for posttranslational modification and trafficking of proteins and lipids. A chloride channel to neutralize the charge of the proton pump and to keep the Golgi membrane potential near zero has finally been discovered.

Full Text - Pores galore for the Golgi | PDF (399 KB) - Pores galore for the Golgi

One hit, two outcomes for VHL-mediated tumorigenesis - pp1127 - 1128

Jürgen Behrens

doi:10.1038/ncb1008-1127

The tumour suppressor VHL is known to suppress hypoxia-induced gene expression by degrading HIF family transcription factors. Evidence that VHL also targets the oncoprotein -catenin for degradation highlights a new road to transformation by loss of VHL.

Full Text - One hit, two outcomes for VHL-mediated tumorigenesis | PDF (278 KB) - One hit, two outcomes for VHL-mediated tumorigenesis

Stressed out? Make some modifications! - pp1129 - 1130

Angela Hilliker & Roy Parker

doi:10.1038/ncb1008-1129

Stress granules and processing bodies are related mRNA-containing granules implicated in controlling mRNA translation and decay. A genomic screen identifies numerous factors affecting granule formation, including proteins involved in O-GlcNAc modifications. These results highlight the importance of post-translational modifications in translational control and mRNP granule formation.

Full Text - Stressed out? Make some modifications! | PDF (356 KB) - Stressed out? Make some modifications!

Dynein branches out - pp1131 - 1132

Guy Tear

doi:10.1038/ncb1008-1131

Individual neurons form specific elaborate dendritic structures that receive presynaptic information. The pattern of dendritic branching is regulated by the microtubule-associated motor protein dynein, which is responsible for the transport of essential endosomes and other organelles into the dendrites.

Full Text - Dynein branches out | PDF (309 KB) - Dynein branches out

Research highlights - p1133

doi:10.1038/ncb1008-1133

Full Text - Research highlights | PDF (224 KB) - Research highlights

GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus - pp1135 - 1145

Yusuke Maeda, Toru Ide, Masato Koike, Yasuo Uchiyama & Taroh Kinoshita

doi:10.1038/ncb1773

This study identifies a voltage-dependent anion channel involved in Golgi acidification, thus providing insights into pH regulation in the Golgi.

Abstract - | Full Text - GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus | PDF (2,217 KB) - GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus | Supplementary information

See also: News and Views by Caldwell & Howell

A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance - pp1146 - 1153

Jia-Shu Yang, Helge Gad, Stella Y. Lee, Alexander Mironov, Leiliang Zhang, Galina V. Beznoussenko, Carmen Valente, Gabriele Turacchio, Akua N. Bonsra, Guangwei Du, Gianluca Baldanzi, Andrea Graziani, Sylvain Bourgoin, Michael A. Frohman, Alberto Luini & Victor W. Hsu

doi:10.1038/ncb1774

Two distinct steps drive COPI vesicle fission: bud-neck constriction, which is dependent on the protein BARS and COPI constituents, followed by bud-neck scission, which is dependent on phosphatidic acid.

Abstract - | Full Text - A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance | PDF (1,509 KB) - A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance | Supplementary information

Proof-by-synthesis of the transcriptional logic of mammalian circadian clocks - pp1154 - 1163

Maki Ukai-Tadenuma, Takeya Kasukawa & Hiroki R. Ueda

doi:10.1038/ncb1775

Regulatory loops between transcriptional activators and repressors control the circadian clock. A minimal synthetic combination of these transcription factors is sufficient to drive a robust circadian rhythm.

Abstract - | Full Text - Proof-by-synthesis of the transcriptional logic of mammalian circadian clocks | PDF (1,912 KB) - Proof-by-synthesis of the transcriptional logic of mammalian circadian clocks | Supplementary information

Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes - pp1164 - 1171

Daisuke Satoh, Daichi Sato, Taiichi Tsuyama, Motoki Saito, Hiroyuki Ohkura, Melissa M. Rolls, Fuyuki Ishikawa & Tadashi Uemura

doi:10.1038/ncb1776

Dendritic morphogenesis is important for neuronal function. In Drosophila melanogaster dendritic arborization neurons, microtubule motors control dendritic branching in a manner that involves trafficking of Rab5-containing early endosomes.

First Paragraph - | Full Text - Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes | PDF (2,253 KB) - Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes | Supplementary information

See also: News and Views by Tear

Dynein is required for polarized dendritic transport and uniform microtubule orientation in axons - pp1172 - 1180

Yi Zheng, Jill Wildonger, Bing Ye, Ye Zhang, Angela Kita, Susan H. Younger, Sabina Zimmerman, Lily Yeh Jan & Yuh Nung Jan

doi:10.1038/ncb1777

Dendrites and axons differ in microtubule orientation and in composition of organelles and protein. In Drosophila melanogaster dendritic arborization neurons, dynein is required for axonal microtubule polarization and for dendrite-specific targeting of Golgi outposts.

First Paragraph - | Full Text - Dynein is required for polarized dendritic transport and uniform microtubule orientation in axons | PDF (2,873 KB) - Dynein is required for polarized dendritic transport and uniform microtubule orientation in axons | Supplementary information

See also: News and Views by Tear

Targeting of the F-actin-binding protein drebrin by the microtubule plus-tip protein EB3 is required for neuritogenesis - pp1181 - 1189

Sara Geraldo, Umme K. Khanzada, Maddy Parsons, John K. Chilton & Phillip R. Gordon-Weeks

doi:10.1038/ncb1778

Plus-tip (+TIP) proteins, such as EB1 and EB3, bind to growing microtubules ends. Interaction of EB3 with drebrin is important to couple filamentous actin to microtubules during neuronal growth-cone formation.

First Paragraph - | Full Text - Targeting of the F-actin-binding protein drebrin by the microtubule plus-tip protein EB3 is required for neuritogenesis | PDF (1,576 KB) - Targeting of the F-actin-binding protein drebrin by the microtubule plus-tip protein EB3 is required for neuritogenesis | Supplementary information

d-Asb11 is an essential mediator of canonical Delta–Notch signalling - pp1190 - 1198

Sander H. Diks, Maria A. Sartori da Silva, Jan-Luuk Hillebrands, Robert J. Bink, Henri H. Versteeg, Carina van Rooijen, Anke Brouwers, Ajay B. Chitnis, Maikel P. Peppelenbosch & Danica Zivkovic

doi:10.1038/ncb1779

The process of lateral inhibition in the canonical Delta-Notch pathway is crucial for cell-fate determination. d-Asb11, a regulator of neural progenitors in zebrafish, is an essential mediator of lateral inhibition through ubiquitylation of Delta A.

First Paragraph - | Full Text - d-Asb11 is an essential mediator of canonical Delta–Notch signalling | PDF (1,234 KB) - d-Asb11 is an essential mediator of canonical Delta–Notch signalling | Supplementary information

The type I TGF- receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner - pp1199 - 1207

Alessandro Sorrentino, Noopur Thakur, Susanne Grimsby, Anders Marcusson, Verena von Bulow, Norbert Schuster, Shouting Zhang, Carl-Henrik Heldin & Maréne Landström

doi:10.1038/ncb1780

In response to TGF, and in contrast to canonical SMAD activation, TGF receptor kinase activity is dispensable for activating the TAK1 kinase, whereas the TRAF6 ubiquitin ligase is neccessary.

First Paragraph - | Full Text - The type I TGF- receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner | PDF (1,346 KB) - The type I TGF- receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner | Supplementary information

Jade-1 inhibits Wnt signalling by ubiquitylating -catenin and mediates Wnt pathway inhibition by pVHL - pp1208 - 1216

Vipul C. Chitalia, Rebecca L. Foy, Markus M. Bachschmid, Liling Zeng, Maria V. Panchenko, Mina I. Zhou, Ajit Bharti, David C. Seldin, Stewart H. Lecker, Isabel Dominguez & Herbert T. Cohen

doi:10.1038/ncb1781

The tumour suppressor VHL is known to stabilize the ubiquitin ligase Jade-1. Jade-1 targets both phosphorylated and unphosphorylated -catenin, thereby linking VHL function directly to inhibition of Wnt signalling.

First Paragraph - | Full Text - Jade-1 inhibits Wnt signalling by ubiquitylating -catenin and mediates Wnt pathway inhibition by pVHL | PDF (1,508 KB) - Jade-1 inhibits Wnt signalling by ubiquitylating -catenin and mediates Wnt pathway inhibition by pVHL | Supplementary information

See also: News and Views by Behrens

The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO₂ - pp1217 - 1223

Miyoung Lee, Yongwook Choi, Bo Burla, Yu-Young Kim, Byeongwook Jeon, Masayoshi Maeshima, Joo-Yeon Yoo, Enrico Martinoia & Youngsook Lee

doi:10.1038/ncb1782

Malate is involved in mediating plant stomatal responses to high CO₂. By acting as a malate importer, the ABC transporter AtABCB14 controls stomatal movements by increasing their osmotic pressure.

First Paragraph - | Full Text - The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO2 | PDF (1,148 KB) - The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO2 | Supplementary information

A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly - pp1224 - 1231

Takbum Ohn, Nancy Kedersha, Tyler Hickman, Sarah Tisdale & Paul Anderson

doi:10.1038/ncb1783

An RNAi sreen shows that assembly of stress granules, which are involved in mRNA translation and decay regulation, requires glycosylation of translation machinery components.

First Paragraph - | Full Text - A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly | PDF (1,713 KB) - A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly | Supplementary information

See also: News and Views by Hilliker & Parker