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The non-specific lethal (NSL) complex is a highly conserved MOF acetyltransferase-containing complex. A recent study now shows that the NSL complex acetylates a new non-histone target, Lamin A/C and reveals the NSL complex as a crucial factor in maintaining nuclear architecture and genome integrity.
Endosomes are central stations for converging proteins from biosynthetic pathways and endocytic routes. Many endosomal proteins are sorted to the plasma membrane or the Golgi despite the lysosome being the primary endosomal fate. SNX5 and SNX6 are now revealed to decode a conserved bipartite signal to mediate protein sorting to the Golgi or the plasma membrane.
Simonetti et al. uncover an endosomal SNX–BAR sorting complex, ESCPE-1, that coordinates sequence-dependent cargo recognition with the formation of tubulo-vesicular transport carriers for endosomal export.
Karoutas et al. report lamin A/C as a non-histone target for the acetyltransferase MOF. They find that lamin A/C acetylation prevents nuclear envelope rupture and maintains nuclear integrity.
Zhao et al. identify an unexpected role of the nuclear export factor Nxf2 as a partner of Panoramix in mediating piRNA-guided silencing. Nxf2 counteracts Nxf1-centred nuclear RNA transport to prevent the export of transposon transcripts.
Levin-Konigsberg et al. show that resorption of the phagolysosome after degradation of its contents requires transfer of PI4P and tethering to the ER, both mediated by oxysterol-binding protein-related protein 1L (ORP1L).
Pessina et al. report that DNA damage induces the assembly of a functional promoter at double-strand breaks and the transcribed RNAs promote phase separation of damage-response factors such as 53BP1.
Cell identity is shaped by a complex interplay between transcription factors, enhancers and genome organisation. A study now reveals a dynamic role for the transcription factor KLF4 in directing gene regulatory interactions during pluripotent cell reprogramming, demonstrating that transcription factors can function as chromatin organisers.
Di Giammartino, Kloetgen, Polyzos, Liu et al. probe chromatin organization, enhancer status and transcriptional changes and show that KLF4 acts as a transcriptional regulator and chromatin organizer during induced pluripotent stem cell reprogramming and in pluripotent stem cells.
Wolf et al. show that N-Ank proteins combine their curvature-sensing ankyrin repeat array and N-terminal amphipathic helix to shape membranes, and ankycorbin shapes membrane protrusions in developing neurons.
Lim et al. show that OSBP and VAPA and VAPB deliver cholesterol across ER–lysosome contacts to activate mTORC1. OSBP-mediated cholesterol trafficking activates mTORC1 in a disease model caused by loss of Niemann–Pick C1.
Li et al. find that IFFO1 bridges the core NHEJ factor XRCC4 and lamin A/C, thus reducing the mobility of broken DNA ends to prevent chromosomal translocation in cancer cells.
He and colleagues develop itChIP-seq based on simultaneous cellular indexing and chromatin tagmentation. itChIP-seq is applicable to both low-input and single-cell analyses of chromatin states.
In this Review, Caridi et al. discuss actin filaments in the nucleus and the functions of nuclear F-actin in response to DNA double-strand break repair.
Trivedi et al. find that phase separation of the chromosome passenger complex is essential for its localization and function at the inner centromere during mitosis.
Assembly of the mitotic spindle requires timely separation of the centrosomes. Their movement apart is driven by the plus-end-directed kinesin Eg5. A new study demonstrates that the kinesin KIFC3 provides an opposing microtubule-based cohesive force that modulates centrosome separation and ensures accurate chromosome segregation.
Tan et al. and Siahaan et al. present distinct but complementary data showing that microtubule regulates dynamic condensation of tau molecules, and this in turn affects microtubule biology and function.
Tan et al. and Siahaan et al. present distinct but complementary data showing that microtubule regulates dynamic condensation of tau molecules, and this in turn affects microtubule biology and function.