Protein variants with improved properties can be rapidly generated by a phage-based system that enables continuous directed evolution.
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References
Esvelt, K.M., Carlson, J.C. & Liu, D.R. Nature 472, 499–503 (2011).
Romero, P.A. & Arnold, F.H. Nat. Rev. Mol. Cell Biol. 10, 866–876 (2009).
Ghadessy, F.J., Ong, J.L. & Holliger, P. Proc. Natl. Acad. Sci. USA 98, 4552–4557 (2001).
Davidson, A.D., Dlugosz, P.J., Levy, M. & Ellington, A.D. Curr. Protoc. Mol. Biol. 87, 24.6.1–24.6.12 (2009).
Tawfik, D.S. & Griffiths, A.D. Nat. Biotechnol. 16, 652–656 (1998).
Ghadessy, F.J. & Holliger, P. Protein Eng. Des. Sel. 17, 201–204 (2004).
Bull, J.J. & Pease, C.M. J. Mol. Evol. 41, 1160–1164 (1995).
Chelliserrykattil, J., Cai, G. & Ellington, A.D. BMC Biotechnol. 1, 13 (2001)
Bloom, J.D. et al. Curr. Opin. Struct. Biol. 15, 447–452 (2005).
Aharoni, A. et al. Nat. Genet. 37, 73–76 (2005).
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Meyer, A., Ellington, A. Molecular evolution picks up the PACE. Nat Biotechnol 29, 502–503 (2011). https://doi.org/10.1038/nbt.1884
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DOI: https://doi.org/10.1038/nbt.1884
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