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Volume 20 Issue 7, July 2002

Nuclear transplantation in process. A bovine oocyte is held by a glass pipette (left),while a glass needle (right) is used to remove the maternal nucleus. A polar body (top) is located just underneath the zona pellucida (the thick envelope surrounding the ovum). Stained with bisbenzimide (Hoeschst 33342; Sigma) dye and visualized under a fluorescence microscope. On page 689, Lanza et al. report the successful transplantation of cloned cells into a steer. Picture courtesy Advanced Cell Technology.

Volume 20 Issue 7

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  • Therapeutic cancer vaccines offer hope for patients for whom traditional treatments have failed, but various obstacles may impede the launch of these new agents.

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  • Therapeutic cloning promises to provide genetically matched replacement tissue, but non-self mitochondrial proteins derived from the recipient oocyte could render cloned tissue immunogenic. A new study examines this issue.

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  • Two reports on whole-genome shuffling demonstrate the application of combinatorial methods for phenotypic improvement in bacteria.

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  • Programmable ribozymes that can attach non-natural amino acids to specific tRNAs may expand the range of amino acids available for protein engineering.

    • Koji Tamura
    • Paul Schimmel
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  • Reporter ribozymes that allow monitoring of protein–nucleic acid and protein–protein interactions facilitate rapid identification of inhibitors for use in drug discovery.

    • Larry Gold
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  • Every year, thousands of researchers head for the US National Institutes of Health. But what draws them there?

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