Volume 49 Issue 3, March 2020

Creating a null mutation of a gene is a powerful way to examine gene function, but knocking out part of a gene does not always result in a null allele.

Far from the Indopacific, aquatics manager Josh Barber and veterinarian Rebecca Ober walk through the recently established dwarf cuttlefish program at Columbia University and share their lessons learned for others interested in these intriguing animals.

As researchers look for more mammalian alternatives, an old moth is learning new tricks.

Goblet cell–associated antigen passages can deliver luminal substances to antigen-presenting cells to induce antigen-specific T cell responses. This protocol describes how to identify and quantify intestinal epithelial cells that have the capacity to take up luminal substances, by intraluminal injection of fluorescent dextran, tissue sectioning for slide preparation and imaging with fluorescence microscopy.