Abstract
The production of mouse chimeras is a common step in the establishment of genetically modified animal strains. Chimeras also provide a powerful experimental tool for following cell behavior during both prenatal and postnatal development. This protocol outlines a simple and economical technique for the production of large numbers of mouse chimeras using traditional diploid morula↔diploid embryonic stem (ES) cell aggregations. Additional steps are included to describe the procedures necessary to produce specialized tetraploid chimeras using tetraploid morula↔diploid ES cell aggregations. This increasingly popular form of chimera produces embryos of nearly complete ES cell derivation that can be used to speed transgenic production or ask developmental questions. Using this protocol, mouse chimeras can be generated and transferred to pseudopregnant surrogate mothers in a 5-d period.
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Acknowledgements
We thank R.R. Behringer, E.H. Lacy and V.E. Papaioannou for advice, discussions and support.
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Supplementary Video 1
Time-lapse movie showing induction of tetraploidy by electrofusion. (MOV 1057 kb)
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Eakin, G., Hadjantonakis, AK. Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos. Nat Protoc 1, 1145–1153 (2006). https://doi.org/10.1038/nprot.2006.173
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DOI: https://doi.org/10.1038/nprot.2006.173
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