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Accurate sequencing by hybridization for DNA diagnostics and individual genomics

Nature Biotechnology volume 16, pages 54–58 (1998)Cite this article

Abstract

Medical DNA diagnostics will increasingly rely on an accurate and inexpensive identification of mutations that affect the function of a gene. To validate diagnostic sequencing by hybridization (SBH), a number of p53 samples were analyzed with the complete set of 8192 noncomplementary 7-mer oligonu-cleotides. In four repeated, blind experiments we accurately sequenced 1.1 kb per each of 12 homozygote and heterozygote samples possessing base substitutions, insertions, and deletions. This SBH variant offers a high throughput platform to inexpensively sequence individual gene or pathogen genome samples within the clinical laboratory setting.

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Author notes

  1. Radoje Drmanac: Corresponding author (e-mail: rade@sbh.com).

Authors and Affiliations

  1. Hyseq Inc., 670 Almanor Ave., Sunnyvale, CA, 94086

    Snezana Drmanac, David Kita, Ivan Labat, Brian Hauser & Radoje Drmanac

  2. Department of Molecular Diagnostics, SmithKline Beecham Pharmaceutical Co., 709 Swedeland Rd., King of Prussia, PA, 19486

    Carl Schmidt & John D. Burczak

Authors
  1. Snezana Drmanac
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  2. David Kita
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  3. Ivan Labat
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  4. Brian Hauser
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  5. Carl Schmidt
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  6. John D. Burczak
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  7. Radoje Drmanac
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Drmanac, S., Kita, D., Labat, I. et al. Accurate sequencing by hybridization for DNA diagnostics and individual genomics. Nat Biotechnol 16, 54–58 (1998). https://doi.org/10.1038/nbt0198-54

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  • DOI: https://doi.org/10.1038/nbt0198-54

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