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Antitumor effects of the MIG and IP-10 genes transferred with poly [D,L-2,4-diaminobutyric acid] on murine neuroblastoma

Cancer Gene Therapy volume 14, pages 696–705 (2007)Cite this article

Abstract

The number of tumor-infiltrating lymphocytes is known to be related to outcomes in patients with a variety of malignancies. Interferon (IFN) γ-inducible protein-10 (IP-10) and monokine induced by IFNγ (MIG) have chemotactic effects on activated T lymphocytes and natural killer (NK) cells. The aim of this study was to evaluate the antitumor effects of exogenous expression of the MIG and IP-10 genes delivered to solid tumors by poly [D,L-2,4-diaminobutyric acid] (PDBA). The murine MIG and IP-10 genes were transfected into mouse neuroblastoma cells with PDBA. MIG and IP-10 levels in supernatants of transfected cells were measured by enzyme-linked immunosorbent assay. The chemotactic activities of MIG and IP-10 in the supernatants of cell cultures were measured by chemotaxis assay. Tumors were injected in vivo with PDBA/pmMIG∷IP-10 complexes to evaluate the effects of these genes on tumor volume and survival time of mice. Transfected PDBA/pmMIG∷IP-10 complexes produced MIG and IP-10 protein in vitro. MIG and IP-10 proteins secreted into the culture medium showed chemotactic activity. MIG and IP-10 gene therapy with the PDBA system in vivo significantly inhibited tumor growth and prolonged survival time of mice. In conclusion, PDBA-mediated MIG and IP-10 gene therapy may be useful for treatment of solid tumors.

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Authors and Affiliations

  1. Department of Surgery I, Oita University Faculty of Medicine, Oita, Japan

    M Tominaga, Y Iwashita, M Ohta, K Shibata, T Ishio & S Kitano

  2. Laboratory of Physical Pharmaceutics, Faculty of Pharmaceutical Sciences, Josai International University, Chiba, Japan

    N Ohmori, T Goto & S Sato

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  1. M Tominaga
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Correspondence to M Tominaga.

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Tominaga, M., Iwashita, Y., Ohta, M. et al. Antitumor effects of the MIG and IP-10 genes transferred with poly [D,L-2,4-diaminobutyric acid] on murine neuroblastoma. Cancer Gene Ther 14, 696–705 (2007). https://doi.org/10.1038/sj.cgt.7701059

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