Letter to the Editor

BJC Open article

British Journal of Cancer (2014) 111, 2203–2204. doi:10.1038/bjc.2014.401 www.bjcancer.com
Published online 12 August 2014

Coexistence of KRAS mutation with mutant but not wild-type EGFR predicts response to tyrosine-kinase inhibitors in human lung cancer

A Choughule1,4, R Sharma1,2,4, V Trivedi1,2,4, A Thavamani1,2, V Noronha1, A Joshi1, S Desai3, P Chandrani2, P Sundaram2, S Utture1, N Jambhekar3, S Gupta1, J Aich2, K Prabhash1 and A Dutt2

  1. 1Department of Medical Oncology, Tata Memorial Hospital, Tata Memorial Center, Mumbai, India
  2. 2Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Center, Navi Mumbai, India
  3. 3Department of Pathology, Tata Memorial Hospital, Tata Memorial Center, Mumbai, India

Correspondence: Dr J Aich or Dr K Prabhash; or Dr A Dutt, E-mail: jyoti.aich@gmail.com or E-mail: kprabhash1@gmail.com or ; E-mail: adutt@actrec.gov.in

4These authors contributed equally to this work.


EGFR and KRAS mutations occur mutually exclusively in NSCLC, suggesting functional redundancy (Kosaka et al, 2004; Pao et al, 2005; Shigematsu et al, 2005; Tam et al, 2006). However, they predict contrasting response rates to tyrosine-kinase inhibitors (TKIs) – while EGFR mutation predicts longer progression-free survival rate (Lynch et al, 2004; Mok et al, 2009; Fukuoka et al, 2011; Chougule et al, 2013), adverse prognosis is associated with patients harbouring KRAS mutations (Mao et al, 2010; Ihle et al, 2012). The recently reported co-occurrence of KRAS and EGFR activating mutations (Li et al, 2014) in 30 of 5125 patients raises questions about the relative values of EGFR and KRAS mutation status as predictors of outcome in NSCLC. This has obvious implications for routine KRAS testing in this disease, potentially precluding EGFR TKI therapy from some patients, similar to the current practice in colorectal cancer (Lievre et al, 2006).

EGFR mutations occur less frequently among Caucasians (10–15%) compared to East Asians (30–60%) (Lynch et al, 2004; Paez et al, 2004) in contrast to KRAS mutations for which the situation is opposite – Caucasians vs East Asians, 25–50% vs 5–15%, respectively (Mao et al, 2010; Roberts et al, 2010). We recently reported an intermediate frequency of EGFR mutations (23%) in a study involving 907 Indian NSCLC patients (Chougule et al, 2013). In a smaller study at our centre there was 74% response to TKI among patients with tumours having EGFR mutations compared to 5% in those with wild-type EGFR (Noronha et al, 2013). We performed directed sequencing of KRAS exons 2 and 3 in 86 patients from the same cohort and correlated its status with outcome after treatment with EGFR TKI (see Supplementary Table S1). There were 15 patients with KRAS G12C and 1 patient with KRAS G12V mutation for an overall mutation rate of 18.6%. Three of these 86 patients had coincident KRAS G12C and EGFR Exon-19 Del (E746-A750) mutations (Table 1), which were independently validated in each of the three samples by four orthogonal technologies (Sequenom Mass Array genotyping, Taqman Real Time PCR, Sanger Sequencing and SNaPShot PCR; see Supplementary Figure 1). All these three patients had partial response to EGFR TKI. However, only 1 of the remaining 13 patients with KRAS mutation had partial response and his tumour had wild-type EGFR with unknown copy number. This is consistent with previous studies: response to EGFR TKI in one of five patients harbouring KRAS mutation with unknown EGFR copy number or mutation status (Zhu et al, 2008); 1 response in 20 KRAS mutant patients who also harboured EGFR amplification but not EGFR mutation (Gumerlock et al, 2005); and response to gefitinib in three of five patients with coincident EGFR and KRAS mutations (Benesova et al, 2010). Furthermore, it has been shown that when KRAS and EGFR mutations are coincident in the same tumour, the genetic lesion in the latter is almost exclusively in exon-19, with virtually no occurrence of exon-21 abnormalities (Li et al, 2014). This was also true in all three cases in our series with coincident EGFR and KRAS mutations. Of note, mutations in exon-19 have been shown to predict a higher response to EGFR TKI than those in exon-21 (Mitsudomi et al, 2005).

In summary, these data suggest that NSCLC patients with KRAS mutations are unlikely to respond to EGFR TKI therapy in the absence of coincident EGFR alterations. Therefore additional KRAS molecular testing may not add predictive value in selecting patients for EGFR TKI therapy and cannot be routinely recommended.



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Supplementary Information accompanies this paper on British Journal of Cancer website

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