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Fast, instrument-free and low-cost selection of high-affinity aptamers

Nature Biotechnology (2023)Cite this article

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We designed a method for fast aptamer selection by integrating biomaterials science, engineering principles and biology. Aptamer candidates dynamically interacting with immobilized targets in a three-dimensional, non-fouling and macroporous polyethylene glycol hydrogel were rapidly enriched and selected with high affinity against five protein targets.

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Fig. 1: Capture of high-affinity aptamers.

References

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This is a summary of: Singh, N. K. et al. High-affinity one-step aptamer selection using a non-fouling porous hydrogel. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01973-8 (2023)

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Fast, instrument-free and low-cost selection of high-affinity aptamers. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01980-9

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