A genetic approach that combines forward genetics with dynamically targeted genome-scale CRISPR–Cas9 tools has been developed to unmask phenotypic variation in Arabidopsis thaliana that is otherwise hidden by functional redundancy. This approach, called Multi-Knock, should be readily deployable by scientists and breeders for basic research and to expedite breeding efforts.
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This is a summary of: Hu, Y. et al. Multi-Knock—a multi-targeted genome-scale CRISPR toolbox to overcome functional redundancy in plants. Nat. Plants https://doi.org/10.1038/s41477-023-01374-4 (2023).
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A genome-scale CRISPR tool for targeting multiple gene family members at once. Nat. Plants 9, 511–512 (2023). https://doi.org/10.1038/s41477-023-01388-y
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DOI: https://doi.org/10.1038/s41477-023-01388-y
