Abstract
Forestation is regarded as an effective strategy for increasing terrestrial carbon sequestration. However, its carbon sink potential remains uncertain due to the scarcity of large-scale sampling data and limited knowledge of the linkage between plant and soil C dynamics. Here, we conduct a large-scale survey of 163 control plots and 614 forested plots involving 25304 trees and 11700 soil samples in northern China to fill this knowledge gap. We find that forestation in northern China contributes a significant carbon sink (913.19 ± 47.58 Tg C), 74% of which is stored in biomass and 26% in soil organic carbon. Further analysis reveals that the biomass carbon sink increases initially but then decreases as soil nitrogen increases, while soil organic carbon significantly decreases in nitrogen-rich soils. These results highlight the importance of incorporating plant and soil interactions, modulated by nitrogen supply in the calculation and modelling of current and future carbon sink potential.
Similar content being viewed by others
Introduction
Forestation (including both afforestation and reforestation) is generally advocated as an effective method to increasing ecosystem carbon sequestartion1. Over the past few decades, forestation has been widely implemented on a global scale, resulting in a substantial expansion of the planted forest area2. Large-scale forestation has been widely reported to improve some ecosystem services, e.g., increasing wood production, soil and water conservation, and enhancing carbon sequestration3,4,5,6,7. For instance, forestation in China has contributed substantially to global greening8 and the recent reversal of the loss of global terrestrial biomass9. However, as one important aspect of ecological consequence of forestation which has attracted broad attention, its carbon sequestration potential remains highly uncertain and controversial1,10,11,12,13. Large-scale field investigations have been scarce, limiting a comprehensive understanding of carbon dynamics after forestation, particularly the interactions between plant biomass and soil carbon dynamics13,14. Therefore, a holistic evaluation of the carbon sequestration induced by forestation at a large scale, which provides foresight and guidelines for the future implementation of forestation projects, is challenging but extremely valuable.
Forestation has the potential to increase carbon sequestration by expanding both plant biomass and soil carbon stock. The increase in biomass carbon storage is a major component of the forestation-induced carbon sink6,13. In similar climate conditions, forests generally hold a larger biomass carbon stock than other ecosystems15, and it has been estimated that the global potential for increased carbon storage in biomass is more than three times that of soil13. In addition to biomass, the second largest carbon sink is soil in the planted forest ecosystem6,13. Although the response of soil carbon to forestation may be slower than the response of biomass, the soil can still strongly regulate the ecosystem carbon balance due to the large carbon stock16. In most studies9,17,18, a fixed ratio between biomass and soil organic carbon (SOC) has generally been used to estimate SOC and ecosystem total carbon stocks based on the strong association between plant biomass and SOC stock under an assumption of proportional carbon dynamics of biomass and SOC. However, an increase in biomass does not necessarily enhance SOC accumulation, and may even result in a SOC loss, as has been observed under CO2 fertilization19,20,21 and in warming systems22. The asymmetry of biomass and soil carbon dynamics may be regulated by climate and soil nutrient supply23, and, in particular, nitrogen20. Unfortunately, this mismatch of carbon dynamics between biomass and SOC after forestation has not yet been thoroughly investigated, hindering our ability to make comprehensive understanding of carbon dynamics after forestation and accurate predictions of the carbon sink potential of forestation.
Here, we conducted a comprehensive, large-scale, paired-survey investigation of the impacts of forestation on biomass and soil carbon stock in northern China. Large-scale afforestation and reforestation programs have been implemented in China since the 1970s24, and currently China has the largest area of planted forests in the world2,25. The Three-North Shelterbelt Development Program (TNSDP), situated in the northeastern, northern and northwestern regions of China, represents the earliest and the most extensive forestation initiative in China26,27. The project covers more than 4,000,000 km2, and is spread across large gradients of climate and soil conditions, providing an ideal opportunity to explore the ecological impacts of forestation in different environments. The pairwise survey campaign was conducted within TNSDP area during 2012 and 2013, and involved 163 control plots and 614 forested plots (Supplementary Fig. 1), with 25,304 trees and 11,700 soil samples being surveyed (see Methods). By using this extensive field dataset, we estimated the carbon sink due to forestation in northern China via a machine learning approach. Furthermore, we compared the dynamics of biomass and SOC after forestation along climate and soil nitrogen gradients to test whether the changes of biomass and SOC were symmetrical (larger biomass means more carbon input to soil) or asymmetrical (plants nutrient acquisition accelerates decomposition of soil organic matter).
Results
Changes in ecosystem carbon sequestration with forestation
Across all the control-forestation plot pairs, the average change of biomass density induced by forestation (Δ(biomass density) = biomass density in forested plot – biomass density in control plot) was 4.80 kg C m−2, indicating a significant (P < 0.001) increase of biomass density after forestation. Δ(biomass density) increased with stand age, with the rate of increase varying across tree species (Fig. 1). Specifically, the largest increases of Δ(biomass density) with stand age were found for Larix (L.) gmelinii (Fig. 1b) and Pinus (P.) sylvestris var. mongholica (Fig. 1c), indicating the fast growth rate of these two tree species. Consequently, forestation with P. sylvestris var. mongholica resulted in the largest increase of biomass density (6.89 kg C m−2), followed by L. gmelinii (5.41 kg C m−2) (Fig. 1f). In contrast, the smallest average Δ(biomass density) was observed for P. tabuliformis (1.40 kg C m−2) (Fig. 1f), which had only a small increase with stand age (Fig. 1d). Δ(biomass density) for Populus spp. increased slightly with stand age (Fig. 1e), with an average value of 4.92 kg C m−2. For P. koraiensis, the slope between Δ(biomass density) and stand age was 0.20 kg C m−2 yr−1 (Fig. 1a), and its average Δ(biomass density) was 4.38 kg C m−2 (Fig. 1f).
The biomass carbon sequestration of forestation also varied among original vegetation and land use type (Supplementary Table 1). The largest carbon sequestration was observed for forestation on natural forest and cropland, especially when forested with P. sylvestris var. mongholica on them. By contrast, the smallest carbon sequestration was observed for forestation on grassland.
To estimate forestation-induced biomass carbon sequestration for the whole study region, a model tree ensemble (MTE) approach was employed (see Methods). Mean annual precipitation (MAP), mean annual temperature (MAT), tree species, stand age, longitude, and latitude were used as the predictors in the MTE. The good performance of this approach (Supplementary Fig. 2) allowed us to generate the distribution of Δ(biomass density) for the whole forestation region of northern China. As shown in Fig. 1g, Δ(biomass density) exhibited high spatial heterogeneity, with a large increase in biomass density in the east of the study region (Jilin province), where large values of planted forest biomass density were measured during the field survey (Supplementary Fig. 1). In the south of the study region, we generally found relatively smaller increases in biomass density induced by forestation, and with some scattered areas (16%) showing decreased biomass density (Fig. 1g).
Based on the planted area for each tree species (Supplementary Table 2, see Methods), forestation in northern China increased biomass carbon stock by 678.25 ± 37.98 Tg C (Table 1). Combining with the increase of SOC (234.94 ± 9.6 Tg C), forestation in northern China increased the total organic carbon (TOC = biomass C + SOC) stock by 913.19 ± 47.58 Tg C. When averaged over the planted area, forestation increased TOC density by 7.42 ± 0.39 kg C m−2, with biomass and SOC contributing 5.51 ± 0.31 kg C m−2 (accounting for 74%) and 1.91 ± 0.08 kg C m−2 (accounting for 26%), respectively.
The forestation-induced carbon sequestration varied strongly across planted tree species due to both divergent changes in carbon density and different planted area (Table 1, Supplementary Table 2). The largest contribution came from L. gmelinii, which increased biomass carbon stock by 236.97 ± 13.93 Tg C, and increased TOC stock by 346.84 ± 15.73 Tg C. Populus spp., which had the largest planted area in northern China, increased the biomass carbon and TOC stocks by 227.36 ± 7.93 Tg C and 287.25 ± 12.23 Tg C, respectively. The group of ‘other’ tree species, was another important contributor of increased TOC stock, accounting for 200.22 ± 13.24 Tg C, with 168.05 ± 11.30 Tg C from biomass. Forestation with P. koraiensis and P. sylvestris var. mongholica increased biomass carbon stocks by only 22.29 ± 1.34 Tg C and 14.96 ± 1.95 Tg C, respectively, due to their small planted areas. P. tabuliformis only increased biomass carbon stocks by 8.61 ± 1.52 Tg C mainly because of its small increase in biomass density. The increase in TOC stocks induced by P. koraiensis, P. sylvestris var. mongholica and P. tabuliformis were 33.55 ± 1.51 Tg C, 19.01 ± 2.16 Tg C and 26.31 ± 2.10 Tg C, respectively.
Divergent responses of biomass and soil organic carbon to forestation
Although forestation increased both biomass and soil stocks, the changes in biomass and SOC were not necessarily synchronous. The ratio of Δ(biomass density) and ΔSOCD had large variation and spatial heterogeneity (Fig. 1h). More surprisingly, biomass and SOC showed contrasting responses to forestation in 29% of pixels. To further explore which environmental factors drive the associations between the biomass and SOC stock in forested ecosystems, we compared the changes in biomass and SOC induced by forestation along the climate and soil nitrogen gradients (Fig. 2). Large increases in biomass density were mostly found in areas with MAT lower than 4 °C (Fig. 2a). In contrast, negative changes in biomass density were found in areas with MAT higher than 8 °C. However, we did not observe similar patterns for the responses of SOC to forestation in the same climate space (Fig. 2b), which implies that the increase (decrease) of plant biomass does not necessarily induce the increase (decrease) of SOC. Combining biomass and SOC, we found that relatively larger TOCD increases were generally distributed in areas with lower MAT ( < 6 °C) (Fig. 2c). However, smaller TOCD increases (even decreases) often occurred in warmer areas (MAT > 8 °C) (Fig. 2c), mainly because of the compensation between biomass and soil C changes (Fig. 2d). Consistent results were observed when aridity index was used in place of MAP (Supplementary Fig. 3).
Soil nitrogen strongly regulates carbon dynamics of both plant and soil20, 23, therefore we also investigated how Δ(biomass density) and ΔSOCD varied with soil total nitrogen density (STND) and stand age (Fig. 2e–h). As shown in Fig. 2e, the larger increases of biomass density induced by forestation were found in areas rich in soil nitrogen and with older stands. In contrast, where soil nitrogen levels were poor and the forest was young, forestation only resulted in a small increase in biomass density, and even a decrease in some areas. The change in SOCD was much smaller than that of biomass density, with different variations along soil nitrogen gradient (Fig. 2f). Specifically, forestation generally decreased SOCD in soils with high nitrogen content, while it increased SOCD in soils with low nitrogen content, especially in older stands. Interestingly, when STND was in the range of 1.0–1.2 kg N m-2 and stand age was more than 40 years, we found that both biomass density and SOCD showed large increases (Fig. 2g). These areas, therefore, had the most pronounced increase of TOCD, indicating that forestation in areas with moderate soil nitrogen can contribute to large values of carbon sequestration after the growth of planted trees.
The foregoing results, based on the outputs of machine learning, indicate the divergent variations between ΔSOCD and Δ(biomass density) along the soil nitrogen gradient, which is further confirmed by the results of field sampling data (Fig. 3). With the increase of STND, Δ(biomass density) increased initially but then gradually decreased, while ΔSOCD decreased monotonically (Fig. 3a). The combination of these two trends induced nonlinear changes of ΔTOCD along soil nitrogen gradient (Fig. 3b). When STND was less than 1.5 kg N m−2, forestation significantly increased TOCD, and the magnitude increased with STND (Fig. 3c). The largest carbon sequestration due to forestation was observed in the group with STND in the range 1–1.5 kg N m−2. When STND exceeded 1.5 kg N m−2, although forestation increased the carbon density in biomass, the loss of SOC offset the increase, so that any changes in TOCD were weak and nonsignificant.
Interestingly, we found a trade-off between SOC and biomass carbon dynamics among different planted tree species (Fig. 3d). For the species with a fast increase of Δ(biomass density) (e.g., L. gmelinii and P. sylvestris var. mongholica), ΔSOCD showed a large decrease along the soil nitrogen gradient. In contrast, for species with a slow increase of Δ(biomass density) (e.g., P. tabuliformis), ΔSOCD only showed a small decrease with increasing STND. These results further suggest that increasing plant biomass may induce soil carbon loss due to nutrient acquisition.
Discussion
This study provides a comprehensive assessment of forestation-induced carbon sequestration through a large-scale pairwise field investigation involving both plant biomass and SOC. Using strict paired design and widespread survey of 25,304 trees and 11,700 soil samples, our study found that forestation in northern China over the past few decades has contributed to a large carbon sink of 913.19 Tg C, with 74% coming from the biomass and 26% from SOC. From the first implementation of TNSDP in 1978 to the sampling year 2012, the mean annual carbon sequestration due to forestation in northern China has reached 26.86 Tg C year-1. Such a value equates to 10.3% of the terrestrial carbon sink in China (0.26 Pg C year−1, based on ref. 25) and 14.9–19.2% of the forest carbon sink in China (0.14–0.18 Pg C year-1, based on ref. 18). These results suggest that forestation can create a considerable carbon sink and is a potential solution to the problem of increasing atmospheric CO2 concentrations.
Our results further indicate that the forestation-induced carbon sink is regulated by many factors, which can provide benchmarks for future forestation. First, we observe that the carbon sequestration capacity varies greatly among tree species, which highlights the importance of the appropriate species choice for forestation. Moreover, multispecies plantations have been found to hold a larger biomass than monocultures28, so the effects of biodiversity and tree species composition should also be carefully evaluated to maximize carbon sink benefits29. Second, local climate and soil conditions also strongly affect the forestation carbon sink. Specifically, forestation in humid (cold) regions sequesters carbon more effectively than in dry (warm) regions (Fig. 2, Supplementary Fig. 3). At the same time, our results reveal that the carbon sink would be larger in regions with moderate nitrogen levels than in regions with high nitrogen levels because of the large carbon loss in nitrogen-rich soils. Therefore, careful and evidence-based site selection and nutrient management are also necessary during forestation campaigns. Third, forest biomass density generally increases rapidly with stand age, especially for newly-planted forests, thus it is reasonable to expect that China’s planted forest will continue to provide a carbon sink in the coming decades30. Indeed, it is estimated that age-related forest biomass C sequestration in China will be 6.69 Pg C (about 0.17 Pg C year-1) from the 2000s to the 2040s, and will be enhanced by ongoing climate change and increasing CO2 concentration31. However, it should be recognized that the carbon sequestration capacity of forest will gradually saturate as stand age increases31,32, so appropriate forestation timing and scientific forest management are also valuable choices. Furthermore, forestation is not panacea to climate change and carbon sequestration is just one of many ecological and social aspects of forestation33. It has been found that simple and unreasonable forestation may also bring both environment and social problems34,35. Therefore, collectively, a sustainable forestation carbon sink requires integrated planning and scientific decision making (to plant or not to plant), accounting for local climates and conditions (where to plant), temporal dynamics of forestation-induced carbon sink (when to plant) and careful species choice and management (how to plant), which are all extremely important if China is to achieve its ‘carbon neutrality’ target.
It is noteworthy that some uncertainties may be involved in our study. For instance, although original vegetation and land use type was found to affect carbon sequestration of forestation, we did not include this variable in our MTE model due to the lack of reliable regional-scale data. However, we conducted further analysis by training MTE models with and without previous land use type involved and found model with vegetation and land use type did not show much better performance (Supplementary Fig. 4), indicating the MTE model used is sufficiently reliable to estimate biomass carbon sink of forestation. In addition, the resolution of data for control groups (300 m) is larger than the size of our sampling plots, which may also bring some uncertainties. Nevertheless, it is acceptable because we have taken efficient efforts to make our sampling plots as representative as possible for the local environment, which can minimize the uncertainties brought by the mismatch in the spatial resolution.
In previous studies on the large-scale carbon balance, SOC stocks were generally estimated as the product of a fixed ratio and biomass carbon storage, due to the scarcity of large-scale SOC measurements9,18. Our pairwise sampling system provides a more accurate understanding of soil carbon dynamics after forestation at the regional scale36, which allows us to further investigate the carbon-dynamics relationships between soil and biomass. Our results indicate that the assumption of a constant ratio between SOC and biomass carbon is unreliable. The reasons for this unreliability may be threefold.
First, changes in biomass and SOC after forestation are asynchronous. In theory, both biomass and SOC would have nonlinear responses to forestation30,37,38. Forest biomass would initially increase with stand age but after some time would reach a relative equilibrium30,38. Here, we found that forestation generally increased biomass carbon density but the equilibrium state was not reached. This may be due to the young forest age and the large spatial heterogeneity among the study sites. Soil carbon dynamics after forestation is ultimately determined by the balance between carbon input and decomposition37,39,40,41, thus it is not necessarily synchronous with changes in biomass carbon. Indeed, forestation-induced change in SOCD was mostly determined by the initial SOC storage, and the magnitude of change gradually decreased with stand age36.
Second, the association between biomass density and SOCD changes along the soil nitrogen gradient, implies that the above- and below-ground interactions are regulated by plant nutrient acquisition. In general, a larger biomass can produce more litter and increase carbon input to the soil carbon pool. Thus, a positive relationship between biomass and SOC is widely used in most terrestrial ecosystem models14,42. However, the increase of plant biomass requires a larger nutrient supply, and can stimulate the decomposition of soil organic matter to obtain more nitrogen43. In areas with a large amount of soil organic matter, the input of litter to the SOC pool cannot completely recharge the strong decomposition of SOC (may be due to the priming effect)44, and hence we observed a large decrease of SOC. Such phenomenon was also observed in tundra, where high plant activity during the growing season stimulates the decomposition of soil organic matter45. Long-term control experiments further found that doubled aboveground litter additions did not increase soil C for any of the forests studied likely due to long-term soil priming46. Changes in soil nitrogen after forestation along the nitrogen gradient support this mechanism (Supplementary Fig. 5), where we found significant decrease of soil nitrogen after forestation in areas rich in soil organic matter47. The combination of increasing carbon input to SOC due to the increase of litter and the increasing decomposition of SOC due to nutrient acquisition after forestation complicates SOC dynamics, and requires further investigation.
Third, the ratio between biomass carbon sequestration and soil carbon sequestration varied greatly among tree species. For instance, the largest ratio between biomass and soil carbon sequestration was observed for ‘other’ group (about 5.2), while the smallest ratio (about 0.5) was observed for P. tabuliformis. Such differences may be caused by the divergent strategies for carbon allocation and nitrogen acquisition among species48. Indeed, we found that tree species with a faster increase in biomass density were generally accompanied by a larger SOC loss along the soil nitrogen gradient (Fig. 3d), implying that the nutrient acquisition strategy is species-specific and strongly associated with plant biomass49.
Forestation regulates the dynamics of SOC via affecting both C inputs and outputs50,51. Besides the litter input and nutrient acquisition (e.g., priming effect), forestation could also regulate SOC dynamics indirectly via changing soil biological or microclimatic conditions (soil temperature, moisture, pH and et.)52,53. Moreover, disturbance and forest management can also affect the input and output of SOC54,55,56. These effects make dynamics of SOC after forestation more complicated and leave the interaction between biomass and soil C cycles more uncertain.
At present, Earth system models (ESMs) generally produce a strong response of SOC to an increase in C input (i.e., NPP)14,42. However, our results suggest that the dynamics of plant C and SOC are not proportionally synergistic and there may be a trade-off between them due to nutrient competition. Such nutrient competition mechanisms have also been observed in tundra forest45, and further confirmed by a synthesized study focusing on CO2 fertilization20. Moreover, both modeling42 and field experiment57 studies have found that increasing N input can enhance soil C sequestration. Given that ESMs are currently inadequate for modeling C and N couples58, a better parameterization and description of C and N interaction schemes is likely to reduce model uncertainties in SOC dynamic simulation. Integrated studies, combining data from manipulative field experiments and large-scale sampling of soil C and N dynamics may yield some new insights, but we suggest that ecological theories of nutrient acquisition can also help to develop and refine the soil C-N schemes used in ESMs.
In summary, via an extensive paired investigation, we estimate that forestation in northern China has contributed a considerable carbon sink of 913.19 ± 47.58 Tg C, 74% of which is from biomass and 26% from SOC. This forestation-induced carbon sink is regulated by many factors, e.g., tree species choice, local climate, soil nutrient supply and stand age, which can provide benchmarks for future forestation on where, when and how to plant. Moreover, we found that biomass and SOC showed divergent responses to forestation along the soil nitrogen gradient, implying a trade-off between biomass and SOC relating to plant nitrogen acquisition. Our results indicate that the wide use of a fixed ratio between biomass and SOC may overestimate the carbon sink potential of forestation, and that the assumption made in most ESMs, that biomass is positively associated with SOC, is not reliable. Therefore, we argue that more experiment results and ecological theories should be used to improve C-N schemes in ESMs.
Methods
Study region
The study region (34.20°–51.80°N and 106.81°–133.31°E) covers seven provinces of northern China (i.e., Heilongjiang, Jilin, Liaoning, Hebei, Shanxi, Shaanxi and the Inner Mongolia) (Supplementary Fig. 1) and has a diverse range of climatic conditions, with MAT ranging from about −8 °C to 16 °C and MAP from about 300 to 1300 mm. The United Nations Food and Agriculture Organization (FAO) soil classification system59,60 lists the dominant soil types in the region as phaeozems, gleysols, humic cambisols, haplic/albic luvisols or eutric/dystric cambisols, haplic calcisols, kastanozems, chernozems, cambisols, haplic alisols, and ferric/haplic luvisols. Soil properties (e.g., pH, organic carbon, and nitrogen) present large gradients in the study area36,52. The region is a hot-spot of forestation in China, containing more than 120,000 km2 of planted forests, most of which are attributed to TNSDP24. It is, therefore, an excellent region for investigating the ecological impacts of forestation under different climate and soil conditions.
Field campaign
The control-forested pairwise system was primarily established to explore the effects of forestation on soil properties36,52,61. The system consisted of 163 study sites, each of which contained a control plot (non-forested plot) and several forested plots (1–26) with different planted tree species and/or different stand age. The distance between any one forested plot and its corresponding non-forested-control plot was usually 50–100 m to minimize any differences in soil and climatic properties between the pair. The largest acceptable distance was 2.5 km, which applied in very few cases. Moreover, the original (pre-forestation) vegetation and land use type and soil type of each forested plot were the same as those of their control plots. The original vegetation and land use types consisted of barren land, cropland, grassland, natural forest and riparian sand land, so both afforestation and reforestation were considered in our study. Note that forestation on natural forests refers to the reforestation of previously clear-cut areas that were originally natural forests. Except cropland, other control groups (barren land, grassland, natural forest and riparian sand land) were not managed. Each control-forested pair, consisting of a forested plot and its corresponding control plot was utilized to provide a good assessment of the impact of forestation. It is also noteworthy that we do not rely on before-and-after comparisons since the control plot could not accurately represent the initial conditions. Instead, we focused on the differences in C stocks between forested versus non-forested areas, all else equal. This approach enables us to evaluate the cost/benefit of forestation accurately. In total, we surveyed 163 control plots and 614 forested plots.
All the forested plots are monoculture, with the dominant tree species being P. koraiensis, L. gmelinii, P. sylvestris var. mongholica, P. tabuliformis and Populus spp. (Populus simonii, Populus beijingensis and Populus×xiaohei). At each forested plot (20 × 20 m2), we measured tree height (TH) and diameter at breast height (DBH) of each tree higher than 2.5 m, and used these data to calculate the biomass. Stand-age data were obtained from local forestry administrations, and were further validated by tree ring observations. In total, 25,304 trees were surveyed.
Soil samples were collected from both control and forested plots. At each plot, we dug three replicate soil profiles on the diagonal and collected samples from various layers (0–5, 5–10, 10–20, 20–30, 30–60 and 60–100 cm). We collected 18 samples in each plot (three replicates × six layers per replicate), except for a few plots which could not be cored to a depth of 1 m. In total, we dug 2331 soil profiles and obtained 11,700 soil samples. Note that we collected two cutting rings of soils at each depth, both of which were identical. One of rings was oven-dried while the other was air-dried in the laboratory. It should be noted that residues, litter, organic layers were not included during the soil sampling process.
Biomass density and its forestation-induced changes
Biomass density in forested plots was calculated based on TH, DBH and the number of trees (NT). First, we calculated the timber volume (Vi) of each tree, based on Eq. (1).
Coefficients (a, e and f) for each tree species can be found in Supplementary Table 3. Equation (1) has been tested, and found to be appropriate, for the tree species in northern China in previous studies and the coefficients have been validated using local field survey data62,63,64,65,66.
Second, we summed the timber volume of all trees in a plot:
and calculated biomass density (B) based on the timber volume (Eq. (3)). This equation is derived from the biomass expansion factor method67.
where A represents the plot area (400 m2), and values for b and c are given in Supplementary Table 4, which is based on ref. 67.
The change in biomass density induced by forestation was derived by subtracting the biomass density in the control plot from the biomass in the paired forested plot. Biomass density in control groups was extracted from the harmonizing vegetation-specific maps of both above and belowground biomass based on the longitude and latitude of each plot68. The datasets comprise both above and belowground biomass for different vegetation types (e.g., cropland, grassland, natural forest al.) at spatial resolution of 300 m in 2010, close to our sampling years (2012 and 2013).
Laboratory work on soil properties
We oven-dried one cutting ring of soils to determine the soil dry weight (SDW) and bulk density. The other cutting ring of soils was air-dried to constant weight in a ventilated room. Roots and stones were removed by passing through 2 mm sieves and the soils were prepared for chemical analyses. Soil total carbon and soil total nitrogen content (STCC and STNC) were measured using an elemental analyzer (Viro el cube, Elementar, Germany). For soil inorganic carbon content (SICC), we used a 08.53 Calcimeter (M1.08.53.E, Eijkelkamp, Netherlands). SOC content (SOCC) was taken as the difference between STCC and SICC. Using SOCC and STNC, soil dry weight (SDW), volume of cutting ring (V), we were able to calculate the SOC and STN densities (SOCD and STND) of each layer in each plot (Eqs. (4) and (5)).
BD indicates soil bulk density, while Ps indicates the volume percentage of soil in each cutting ring (100% minus the volume percentage of roots and stones). In the equations, j indicates the jth layer, and w indicates the thickness of the layer, e.g., j = 1 indicate the first layer (0–5 cm, w = 5) while j = 6 indicates the sixth layer (60–100 cm, w = 40). Note that each plot had three replicate profiles, so mean STNDj and SOCDj for the three profiles were used in the analysis. The sum of mean STNDj and SOCDj in all layers was used to represent STN and SOC densities in each plot.
The difference of SOC between control and forested plots were used to indicate the impacts of forestation on SOC, which was then upscaled in the whole study region using boosted regression trees36.
Statistical analyses and upscaling estimation
In this study, data analyses were conducted based on control-forested pairs. The independent student’s t test was used to explore whether the change in biomass density (Δ(biomass density) = biomass density in forested plot - biomass density in control plot) induced by forestation was significantly different from 0. False discovery rates were corrected to control potential error rates in multiple comparisons69. Linear mixed models were performed to determine the relationships between carbon changes and stand age (Fig. 1) and STND (Fig. 3), where site number was added into the models as a random factor.
To upscale forestation-induced changes in biomass density, we applied an MTE approach. This method was first discussed by Jung et al70. and has since been widely used to upscale carbon (water) flux and storage to large spatial scales71,72,73. To avoid overfitting, we randomly selected 80% of the sample data to form the training dataset, with the remaining 20% forming the validation dataset. The model trees were trained at the plot-level using MAP, MAT, tree species, stand age, longitude, and latitude as model inputs. Note that we used the change in biomass density induced by forestation as the dependent variable, so that we could estimate the carbon sequestration of forestation directly. The trained MTE is then applied to the validation dataset to test the performance of the MTE in simulating changes in biomass density. The output estimation is the median of 20 independent best ensemble members. We obtained changes in biomass density estimation in each 1 km spatial resolution grid cell by applying the well-trained MTE. The spatial distribution of planted forests at 1-km resolution was obtained from the report of the Seventh National Forest Resource Inventory (2004–2008), released by the State Forestry Administration of China53. Tree species data were derived from the 1:1,000,000 vegetation map of China74. MAP and MAT were obtained from the China Meteorological Forcing Dataset, which was created by merging a variety of data sources, and has a spatial resolution of 0.1 × 0.1° and a temporal resolution of 3 h75,76. Stand-age data were derived from a forest age map of China77, which was developed by downscaling the national forest inventory data to a 1 km spatial resolution. Data of aridity index were derived from global aridity index and potential evapo-transpiration (ET0) database v378, which were used to validate the results based on MAP. In addition, data of soil carbon and nitrogen and biomass, and code related to this study have been deposited at figshare79,80,81.
Importantly, the map of planted forests may not perfectly match the actual planted area for the study region, so the total regional change in biomass was calculated by weighting the simulated mean change in biomass density for tree group j in province i (Xij) by its corresponding planted area (Aij) (Eq. (6)). The planted area for each tree species in each province is shown in Supplementary Table 2. Note the value of X for the ‘other’ tree species group was set to the mean value of the five major tree species.
Finally, we combined the estimated Δ(biomass density) and total change in biomass with changes in SOC density (i.e., ΔSOCD) and total change in SOC (see ref. 36 for SOC) to calculate the forestation-induced changes in total organic carbon density (i.e., TOCD) and stocks.
Data availability
The field data of soil C and N related to this research have been deposited at figshare (https://doi.org/10.6084/m9.figshare.21341577). Data of biomass in planted forests and other related data have been deposited at figshare (https://doi.org/10.6084/m9.figshare.22346332). Data of aridity index are openly available at https://doi.org/10.6084/m9.figshare.7504448.v5. Data of biomass for control group are derived from https://doi.org/10.3334/ORNLDAAC/1763.
Code availability
The code used in this research are available at figshare (https://doi.org/10.6084/m9.figshare.22346344).
References
Bastin, J. F. et al. The global tree restoration potential. Science 365, 76–79 (2019).
FAO. Global Forest Resources Assessment 2015. (Food and Agricultural Organization of the United Nations, 2016).
Fang, J. & Chen, A. Dynamic forest biomass carbon pools in China and their significance. Acta Botanica Sin. 43, 967–973 (2001).
Fang, J. et al. Forest biomass carbon sinks in East Asia, with special reference to the relative contributions of forest expansion and forest growth. Glob. Change Biol. 20, 2019–2030 (2014).
Ouyang, Z. et al. Improvements in ecosystem services from investments in natural capital. Science 352, 1455–1459 (2016).
Lu, F. et al. Effects of national ecological restoration projects on carbon sequestration in China from 2001 to 2010. Proc. Natl Acad. Sci. USA 115, 4039–4044 (2018).
Lu, N. et al. Biophysical and economic constraints on China’s natural climate solutions. Nat. Clim. Change 12, 847–853 (2022).
Chen, C. et al. China and India lead in greening of the world through land-use management. Nat. Sustain. 2, 122–129 (2019).
Liu, Y. et al. Recent reversal in loss of global terrestrial biomass. Nat. Clim. Change 5, 470–474 (2015).
Griscom, B. et al. Natural climate solutions. Proc. Natl Acad. Sci. USA 114, 11645–11650 (2017). 44.
Lewis, S. et al. Comment on “The global tree restoration potential”. Science 366, 6463 (2019).
Veldman, J. et al. Comment on “The global tree restoration potential”. Science 366, 6463 (2019).
Walker, W. et al. The global potential for increased storage of carbon on land. Proc. Natl Acad. Sci. USA 19, e2111312119 (2022).
Tian, H. et al. Global patterns and controls of soil organic carbon dynamics as simulated by multiple terrestrial biosphere models: current status and future directions. Glob. Biogeochem. Cycles 29, 775–792 (2015).
Arevalo, C., Bhatti, J., Chang, S. & Sidders, D. Ecosystem carbon stocks and distribution under different land-uses in north central Alberta, Canada. For. Ecol. Manag. 257, 1776–1785 (2009).
Lal, R. Soil carbon sequestration impacts on global climate change and food security. Science 304, 1623–1627 (2004).
Shvidenko, A. & Nilsson, S. A synthesis of the impact of Russian forests on the global carbon budget for 1961–1998. Tellus 55B, 391–415 (2003).
Pan, Y. et al. A large and persistent carbon sink in the world’s forests. Science 333, 988–993 (2011).
Hoosbeek, M. et al. More new carbon in the mineral soil of a poplar plantation under free air carbon enrichment (POPFACE): cause of increased priming effect? Global Biogeochem. Cycles 18. https://doi.org/10.1029/2003GB002127 (2004).
Terrer, C. et al. A trade-off between plant and soil carbon storage under elevated CO2. Nature 591, 599–603 (2021).
Maschler, J. et al. Links across ecological scales: Plant biomass responses to elevated CO2. Glob. Change Biol. 28, 6115–6134 (2022).
Lu, M. et al. Responses of ecosystem carbon cycle to experimental warming: a meta-analysis. Ecology 94, 726–738 (2013).
Hyvönen, R. et al. The likely impact of elevated [CO2], nitrogen deposition, increased temperature and management on carbon sequestration in temperate and boreal forest ecosystems: a literature review. N. Phytol. 173, 463–480 (2006).
State Forestry Administration of the People’s Republic of China, Eighth National Forest Resource Inventory Report (2009–2013), http://www.forestry.gov.cn/main/65/20140225/659670.html (2014).
Piao, S. et al. The carbon balance of terrestrial ecosystems in China. Nature 458, 1009–1013 (2009).
He, B., Chen, A., Wang, H. & Wang, Q. Dynamic response of satellite-derived vegetation growth to climate change in the Three North Shelter Forest region in China. Remote Sens 7, 9998–10016 (2015a).
He, B. et al. Carbon sequestration from China’s afforestation projects. Environ. Earth Sci. 74, 5491–5499 (2015b).
Feng, Y. et al. Multispecies forest plantations outyield monocultures across a broad range of conditions. Science 376, 865–868 (2022).
Hua, F. et al. The biodiversity and ecosystem service contributions and trade-offs of forest restoration approaches. Science 376, 839–844 (2022).
He, N. et al. Vegetation carbon sequestration in Chinese forests from 2010 to 2050. Glob. Change Biol. 23, 1575–1584 (2017).
Yao, Y., Piao, S. & Wang, T. Future biomass carbon sequestration capacity of Chinese forests. Sci. Bull. 63, 1108–1117 (2018a).
Gunderson, P. et al. Old-growth forest carbon sinks overestimated. Nature 591, E21 (2021).
Lewis, S. et al. Regenerate natural forests to store carbon. Nature 568, 25–28 (2019).
Cao, S. et al. Excessive reliance on afforestation in China’s arid and semi-arid regions: Lessons in ecological restoration. Earth-Sci. Rev. 104, 240–245 (2011).
Fleischmann, F. et al. Pitfalls of tree planting show why we need people-centered natural climate solutions. BioScience 70, 947–950 (2020).
Hong, S. et al. Divergent responses of soil organic carbon to afforestation. Nat. Sustain. 3, 694–700 (2020).
Li, W. et al. Temporal response of soil organic carbon after grassland-related land-use change. Glob. Change Biol. 24, 4731–4746 (2018).
Bukoski, J. et al. Rates and drivers of aboveground carbon accumulation in global monoculture plantation forests. Nat. Commun. 13, 4206 (2022).
Soong, J. et al. Five years of whole-soil warming led to loss of subsoil carbon stocks and increased CO2 efflux. Sci. Adv. 7, eabd1343 (2021).
Jobbágy, E. & Jackson, R. The Vertical Distribution of Soil Organic Carbon and Its Relation to Climate and Vegetation. Ecol. Appl. 10, 423–436 (2020).
Cardon, Z. et al. Contrasting effects of elevated CO2 on old and new soil carbon pools. Soil Biol. Biochem. 33, 365–373 (2001).
Todd-Brown, K. E. O. et al. Causes of variation in soil carbon simulations from CMIP5 Earth system models and comparison with observations. Biogeosciences 10, 1717–1736 (2013).
Brady, N. & Weil, R. The nature and properties of soils. (Prentice-Hall Press, New York, 2008).
Kuzyakov, Y. & Gavrichkova, O. Time lag between photosynthesis and carbon dioxide efflux from soil: a review of mechanisms and controls. Glob. Change Biol. 16, 3386–3406 (2010).
Hartley, I. et al. A potential loss of carbon associated with greater plant growth in the European Arctic. Nat. Clim. Change 2, 875–879 (2012).
Castañeda-Gómez, L. et al. Soil organic matter molecular composition with long-term detrital alterations is controlled by site-specific forest properties. Glob. Change Biol. 29, 243–259 (2023).
Hong, S. et al. Effects of afforestation on soil carbon and nitrogen accumulation depend on initial soil nitrogen status. Glob. Biogeochem. Cycles 37, e2022GB007490 (2023).
Deng, M. et al. Ecosystem scale trade-off in nitrogen acquisition pathways. Nat. Ecol. Evol. 2, 1724 (2018).
Yin, L. et al. Rhizosphere priming effects on soil carbon and nitrogen dynamics among tree species with and without intraspecific competition. N. Phytologist 218, 1036–1048 (2018).
Binkley D. & Fisher R. Ecology and Management of Forest Soils 4th edn (Wiley-Blackwell, 2013).
Davidson, E. & Janssens, I. Temperature sensitivity of soil carbon decomposition and feedbacks to climate change. Nature 440, 165–173 (2006).
Hong, S. et al. Afforestation neutralizes soil pH. Nat. Commun. 9, 520 (2018).
Peng, S. et al. Afforestation in China cools local land surface temperature. Proc. Natl Acad. Sci. USA 111, 2915–2919 (2014).
Laganière, J. et al. Carbon accumulation in agricultural soils after afforestation: a meta-analysis. Glob. Change Biol. 16, 439–453 (2010).
Wang, W. et al. Changes in soil organic carbon, nitrogen, pH and bulk density with the development of larch (Larix gmelinii) plantations in China. Glob. Change Biol. 17, 2657–2676 (2011).
Mayer, M. et al. Tamm Review: Influence of forest management activities on soil organic carbon stocks: A knowledge synthesis. Forest Ecol. Manag. 466, 118127 (2020).
Lu, X., Vitousek, P. M., Mao, Q., Gilliam, F. S. & Mo, J. Nitrogen deposition accelerates soil carbon sequestration in tropical forests. Proc. Natl Acad. Sci. USA 118, e2020790118 (2021).
Wutzler, T. & Reichstein, M. Colimitation of decomposition by substrate and decomposers – a comparison of model formulations. Biogeoscience 5, 749–759 (2008).
FAO, IASA, ISRIC, ISSCAS, & JRC. Harmonized world soil database (version 1.2). (FAO and IIASA, 2012).
Xiong, Y. & Li, Q. Soils in China. (Press of Sciences, 1987).
Hong, S. & Chen, A. Contrasting responses of soil inorganic carbon to afforestation in acidic versus alkaline soils. Glob. Biogeochem. Cycles 36, e2021GB007038 (2022).
Wang, Y. & Wu, F. Study on the compilation of timber volume table of Pinus tabulaeformis plantation in Fuxin. J. Northeast Forestry Univ. 15, 5 (1987).
Fang, C. et al. Construction of bivariate stem volume tables of poplar in Chaoyang region. J. Liaoning Forestry Sci. Technol. 4, 5–7 (2001a).
Gong, S. & Liu, D. Establishment of the Tree Volume Tables of Pinus Sylvestris var mongolica Plantation in Daxinganling Region. Forest Resourc. Manag. 5, 20 (2002).
Yang, H. et al. Reformulation of binary timber volume table of artificial Korean pine in Liaoning Province. J. Liaoning Forestry Sci. Technol. 2, 61 (2007).
Song, Y. Establishment of binary tree volume table of Larix gmellini (Rupr.) Rupr plantation. Forestry Prospect Design 1, 59–60 (2012).
Fang, J., Chen, A., Peng, C., Zhao, S. & Ci, L. Changes in Forest Biomass Carbon Storage in China Between 1949 and 1998. Science 292, 2320–2322 (2001b).
Spawn, S. et al. Harmonized global maps of above and belowground biomass carbon density in the year 2010. Sci. Data 7, 112 (2020).
Benjamini, Y. & Yekutieli, D. The control of false discovery rate in multiple testing under dependency. Ann. Stat. 4, 1165–1188 (2001).
Jung, M., Reichstein, M. & Bondeau, A. Towards global empirical upscaling of FLUXNET eddy covariance observations: validation of a model tree ensemble approach using a biosphere model. Biogeoscience 6, 2001–2013 (2009).
Jung, M. et al. Recent decline in the global land evapotranspiration trend due to limited moisture supply. Nature 467, 951–954 (2010).
Zeng, Z. et al. A worldwide analysis of spatiotemporal changes in water balance-based evapotranspiration from 1982 to 2009. J. Geophys. Res. Atmos. 119, 1186–1202 (2014).
Yao, Y. et al. A new estimation of China’s net ecosystem productivity based on eddy covariance measurements and a model tree ensemble approach. Agric. For. Meteorol. 253–254, 84–93 (2018b).
Hou, X. Vegetation Atlas of China (1: 1,000,000). (Science Press. (in Chinese), 2001).
Yang, K., He, J., Tang, W., Qin, J. & Cheng, C. On downward shortwave and longwave radiations over high altitude regions: Observation and modeling in the Tibetan Plateau. Agric. Meteorol. 150, 38–46 (2010).
Chen, Y. et al. Improving land surface temperature modeling for dry land of China. J. Geophys. Res. Atmos. 116, 999–1010 (2011).
Zhang, Y., Yao, Y., Wang, X., Liu, Y. & Piao, S. Mapping spatial distribution of forest age in China. Earth Space Sci. 4, 108–116 (2017).
Zomer, R., Xu, J. & Trabucco, A. Version 3 of the Global Aridity Index and Potential Evapotranspiration Database. Sci. Data 9, 409 (2022).
Hong, S. et al. Biomass data for the article entitled “Asymmetry of carbon sequestrations by plant and soil after forestation regulated by soil nitrogen”. Figshare https://doi.org/10.6084/m9.figshare.22346332 (2023).
Hong, S. et al. Soil data for the article entitled “Asymmetry of carbon sequestrations by plant and soil after forestation regulated by soil nitrogen”. Figshare https://doi.org/10.6084/m9.figshare.21341577 (2022).
Hong, S. et al. Code for the article entitled “Asymmetry of carbon sequestrations by plant and soil after forestation regulated by soil nitrogen”. Figshare https://doi.org/10.6084/m9.figshare.22346344 (2023).
Acknowledgements
This study was supported by the National Key R&D Program of China (2019YFA0607304) and and the Chinese Academy of Sciences (CAS) Project for Young Scientists in Basic Research (YSBR-037). S.H. acknowledges the support from the Postdoctoral Innovation Talents Support Program of China (Grant No. BX2021005).
Author information
Authors and Affiliations
Contributions
S.P., S.H., and J.D. designed the study. S.H. conducted the experiments, performed the analysis and drafted the figures. S.H. and J.D. drafted the paper. S.H., J.D., F.K., H.X., S.C. Y.Y., and S.P. contributed to the interpretation of the results and to the text.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Peer review
Peer review information
Nature Communications thanks David Paré and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. A peer review file is available.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Hong, S., Ding, J., Kan, F. et al. Asymmetry of carbon sequestrations by plant and soil after forestation regulated by soil nitrogen. Nat Commun 14, 3196 (2023). https://doi.org/10.1038/s41467-023-38911-w
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41467-023-38911-w
This article is cited by
-
Comparative Analysis of the Drivers of Soil Organic Carbon, Total Nitrogen, and Phosphorus Stocks in Different Coniferous Plantations on the Eastern Tibetan Plateau
Journal of Soil Science and Plant Nutrition (2024)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.