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MiR-4295 facilitates cell proliferation and metastasis in head and neck squamous cell carcinoma by targeting NPTX1

Genes & Immunity volume 21, pages 4–12 (2020)Cite this article

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Abstract

It has been reported that MicroRNAs (miRNAs) play pivotal roles in the occurrence and progression of a variety of cancers. As reported, miR-4295 promotes cell growth and metastasis in a lot of cancers. Nonetheless, the role and molecular mechanism of miR-4295 in HNSCC still remain unknown. In this study, we discovered miR-4295 expression was significantly upregulated in HNSCC tissues and cell lines, which is also associated with the overall survival of patients. Additionally, suppression of miR-4295 significantly inhibited cell proliferation, migration and EMT process in HNSCC. Through Targetscan website, it was predicted that NPTX1 might be a direct target gene of miR-4295. Then, we verified that NPTX1 could directly interact with miR-4295 via luciferase reporter and RNA assays. What’s more, we discovered that there was a significantly negative correlation between NPTX1 and miR-4295 expression. It was indicated by further investigation that the effect of miR-4295 suppression on cell proliferation, migration and EMT process in HNSCC can be restored by knockdown of NPTX1 at the same time. Our results suggested that miR-4295 promoted the progression of HNSCC via regulating NPTX1 expression and miR-4295/NPTX1 axis, which may be a new therapeutic strategy for HNSCC.

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References

  1. Guo T, Califano JA. Molecular biology and immunology of head and neck cancer. Surg Oncol Clin N Am. 2015;24:397–407.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin. 2012;62:10–29.

    Article  PubMed  Google Scholar 

  3. Cramer JD, Speedy SE, Ferris RL, Rademaker AW, Patel UA, Samant S. National evaluation of multidisciplinary quality metrics for head and neck cancer. Cancer. 2017;123:4372–81.

    Article  PubMed  Google Scholar 

  4. Jou A, Hess J. Epidemiology and molecular biology of head and neck cancer. Oncol Res Treat. 2017;40:328–32.

    Article  CAS  PubMed  Google Scholar 

  5. Hayes J, Peruzzi PP, Lawler S. MicroRNAs in cancer: biomarkers, functions and therapy. Trends Mol Med. 2014;20:460–9.

    Article  CAS  PubMed  Google Scholar 

  6. Srivastava SK, Bhardwaj A, Leavesley SJ, Grizzle WE, Singh S, Singh AP. MicroRNAs as potential clinical biomarkers: emerging approaches for their detection. Biotech Histochem. 2013;88:373–87.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Croce CM. Causes and consequences of microRNA dysregulation in cancer. Nat Rev Genet. 2009;10:704–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Guo Z, Li J, Sun J, Sun L, Zhou Y, Yu Z. miR-346 Promotes HCC Progression by Suppressing Breast Cancer Metastasis Suppressor 1 Expression. Oncol Res. 2018;26:1073–81.

    Article  PubMed  Google Scholar 

  9. Li G, Wu F, Yang H, Deng X, Yuan Y. MiR-9-5p promotes cell growth and metastasis in non-small cell lung cancer through the repression of TGFBR2. Biomed Pharm. 2017;96:1170–8.

    Article  CAS  Google Scholar 

  10. Cao XC, Yu Y, Hou LK, Sun XH, Ge J, Zhang B, et al. miR-142-3p inhibits cancer cell proliferation by targeting CDC25C. Cell Prolif. 2016;49:58–68.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Sripada L, Singh K, Lipatova AV, Singh A, Prajapati P, Tomar D, et al. hsa-miR-4485 regulates mitochondrial functions and inhibits the tumorigenicity of breast cancer cells. J Mol Med. 2017;95:641–51.

    Article  CAS  PubMed  Google Scholar 

  12. Obayashi M, Yoshida M, Tsunematsu T, Ogawa I. microRNA-203 suppresses invasion and epithelial-mesenchymal transition induction via targeting NUAK1 in head and neck cancer. Oncotarget. 2016;7:8223–39.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Nakanishi H, Taccioli C, Palatini J, Fernandez-Cymering C, Cui R, Kim T, et al. Loss of miR-125b-1 contributes to head and neck cancer development by dysregulating TACSTD2 and MAPK pathway. Oncogene. 2014;33:702–12.

    Article  CAS  PubMed  Google Scholar 

  14. Zhao Y, Ling Z, Hao Y, Pang X. MiR-124 acts as a tumor suppressor by inhibiting the expression of sphingosine kinase 1 and its downstream signaling in head and neck squamous cell carcinoma. Oncotarget. 2017;8:25005–20.

    PubMed  PubMed Central  Google Scholar 

  15. Nan Y-H, Wang J, Wang Y, Sun P-H. MiR-4295 promotes cell growth in bladder cancer by targeting BTG1. Am J Transl Res. 2016;8:4892.

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Shao M, Geng Y, Lu P, Xi Y, Wei S, Wang L, et al. miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A. Biochem Biophys Res Commun. 2015;464:1309–13.

    Article  CAS  PubMed  Google Scholar 

  17. Huang R, Zong X. Aberrant cancer metabolism in epithelial-mesenchymal transition and cancer metastasis: mechanisms in cancer progression. Crit Rev Oncol Hematol. 2017;115:13–22.

    Article  PubMed  Google Scholar 

  18. Diepenbruck M, Christofori G. Epithelial-mesenchymal transition (EMT) and metastasis: yes, no, maybe? Curr Opin Cell Biol. 2016;43:7–13.

    Article  CAS  PubMed  Google Scholar 

  19. Peng X, Pan K, Zhao W, Zhang J. NPTX1 inhibits colon cancer cell proliferation through down-regulating cyclin A2 and CDK2 expression. Cell Biol Int. 2018;42:589–597.

    Article  CAS  Google Scholar 

  20. Zhou C, Qin Y, Xie Z, Zhang J, Yang M, Li S, et al. NPTX1 is a novel epigenetic regulation gene and associated with prognosis in lung cancer. Biochem Biophys Res Commun. 2015;458:381–6.

    Article  CAS  PubMed  Google Scholar 

  21. Setijono SR, Park M, Kim G, Kim Y, Cho KW, Song SJ. miR-218 and miR-129 regulate breast cancer progression by targeting Lamins. Biochem Biophys Res Commun. 2018;496:826–33.

    Article  CAS  PubMed  Google Scholar 

  22. Zhou W, He L, Dai Y, Zhang Y, Wang J, Liu B. MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer. Exp Ther Med. 2018;16:2811–20.

    PubMed  PubMed Central  Google Scholar 

  23. Chapman BV, Wald AI, Akhtar P, Munko AC, Xu J, Gibson SP, et al. MicroRNA-363 targets myosin 1B to reduce cellular migration in head and neck cancer. BMC Cancer. 2015;15:861.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Zhang S, Liu Z, Wu L, Wang Y. MiR-361 targets Yes-associated protein (YAP) mRNA to suppress cell proliferation in lung cancer. Biochem Biophys Res Commun. 2017;492:468–73.

    Article  CAS  PubMed  Google Scholar 

  25. Li D, Song H, Wu T, Xie D, Hu J, Zhao J, et al. MiR-519d-3p suppresses breast cancer cell growth and motility via targeting LIM domain kinase 1. Mol Cell Biochem. 2018;444:169–78.

    Article  CAS  PubMed  Google Scholar 

  26. Xu G, Zhang Z, Zhang L, Chen Y, Li N, Lv Y, et al. miR-4326 promotes lung cancer cell proliferation through targeting tumor suppressor APC2. Mol Cell Biochem. 2018;443:151–7.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors appreciate all laboratory members. This study was supported by Grant agency of the Science and Technology Department of Sichuan Province (No. 2017HH0096).

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Authors and Affiliations

  1. Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China

    Shun Lu, Hanyi Zhang & Mei Feng

  2. Department of Radioation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China

    Cheng Zhou

  3. Department of Thoracic Oncology, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Chengdu, 610041, China

    Bingwen Zou

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  1. Shun Lu
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Correspondence to Shun Lu.

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Lu, S., Zhou, C., Zou, B. et al. MiR-4295 facilitates cell proliferation and metastasis in head and neck squamous cell carcinoma by targeting NPTX1. Genes Immun 21, 4–12 (2020). https://doi.org/10.1038/s41435-019-0081-0

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