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A new polycyclic tetramate macrolactam from Allostreptomyces RD068384: stereochemistry and antifungal potential

The Journal of Antibiotics volume 77, pages 393–396 (2024)Cite this article

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Abstract

A new polycyclic tetramate macrolactam designated allostreptamide (1), together with four known congeners, were isolated from the culture extract of Allostreptomyces RD068384. The planar structure of the new compound was elucidated through interpretation of NMR and MS data. The absolute configuration was determined through ROESY and ECD analyses. The isolated compounds revealed antifungal potential against fourteen Candida albicans isolates with minimum inhibitory concentrations (MICs) ranging from 64 to 2048 µg ml-1. Compound 3 showed antibiofilm action and considerably reduced the viability of five isolates (36%) in the formed biofilm. The qRT-PCR revealed that 3 downregulated the BCR1, PLB2, ALS1, and SAP5 biofilm related gene expression. Therefore, 3 could be a promising antifungal therapy for C. albicans infections.

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Authors and Affiliations

  1. Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt

    Marwa Elsbaey

  2. Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan

    Marwa Elsbaey, Yuki Samaru, Naoya Oku & Yasuhiro Igarashi

  3. Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt

    Engy Elekhnawy

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  1. Marwa Elsbaey
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  2. Yuki Samaru
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Correspondence to Marwa Elsbaey.

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Elsbaey, M., Samaru, Y., Elekhnawy, E. et al. A new polycyclic tetramate macrolactam from Allostreptomyces RD068384: stereochemistry and antifungal potential. J Antibiot 77, 393–396 (2024). https://doi.org/10.1038/s41429-024-00705-7

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