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New chloptosins B and C from an Embleya strain exhibit synergistic activity against methicillin-resistant Staphylococcus aureus when combined with co-producing compound L-156,602

The Journal of Antibiotics volume 74, pages 80–85 (2021)Cite this article

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A Correction to this article was published on 17 May 2024

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Abstract

Two new dimeric cyclohexapeptides, chloptosins B and C, were discovered from the culture broth of Embleya sp. MM621-AF10 together with the known compounds chloptosin and L-156,602. The structures of the new chloptosins were determined by spectroscopic studies and advanced Marfey’s methods. The stereo structure of the constituent isoleucine was determined by C3 Marfey’s analysis. Chloptosins demonstrated potent antimicrobial activity against Gram-positive bacteria including drug-resistant strains of methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci with MICs of 0.5–2 µg ml−1. The antimicrobial activities of chloptosins were enhanced by addition of co-producing compound L-156,602, as shown by checkerboard analysis.

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Acknowledgements

The authors are grateful to Ms Yuko Takahashi at the Institute of Microbial Chemistry (BIKAKEN), Tokyo, for the LC-MS measurements. We thank Renee Mosi, PhD, from Edanz Group (https://en-author-services.edanzgroup.com/) for editing a draft of this manuscript.

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Authors and Affiliations

  1. Laboratory of Microbiology, Institute of Microbial Chemistry (BIKAKEN), 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo, 141-0021, Japan

    Hideki Hashizume, Shigeko Harada, Yuko Shibuya, Ryoko Nagasaka, Masaki Hatano & Masayuki Igarashi

  2. Laboratory of Molecular Structure Analysis, Institute of Microbial Chemistry (BIKAKEN), 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo, 141-0021, Japan

    Ryuichi Sawa, Kiyoko Iijima & Yumiko Kubota

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  1. Hideki Hashizume
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Correspondence to Hideki Hashizume.

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The original online version of this article was revised: It has come to the attention of the authors that there was an error in Figure 1 of the original article. Specifically, the depiction of the carboxylic acid group of threonine in the right half of the structures of chloptosins was missing. The correct figure is below. The original article has been corrected.

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Hashizume, H., Harada, S., Sawa, R. et al. New chloptosins B and C from an Embleya strain exhibit synergistic activity against methicillin-resistant Staphylococcus aureus when combined with co-producing compound L-156,602. J Antibiot 74, 80–85 (2021). https://doi.org/10.1038/s41429-020-0361-y

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