Abstract
Enhancing endocannabinoid signaling produces anxiolytic- and antidepressant-like effects, but the neural circuits involved remain poorly understood. The medial habenula (MHb) is a phylogenetically-conserved epithalamic structure that is a powerful modulator of anxiety- and depressive-like behavior. Here, we show that a robust endocannabinoid signaling system modulates synaptic transmission between the MHb and its sole identified GABA input, the medial septum and nucleus of the diagonal band (MSDB). With RNAscope in situ hybridization, we demonstrate that key enzymes that synthesize or degrade the endocannabinoids 2-arachidonylglycerol (2-AG) or anandamide are expressed in the MHb and MSDB, and that cannabinoid receptor 1 (CB1) is expressed in the MSDB. Electrophysiological recordings in MHb neurons revealed that endogenously-released 2-AG retrogradely depresses GABA input from the MSDB. This endocannabinoid-mediated depolarization-induced suppression of inhibition (DSI) was limited by monoacylglycerol lipase (MAGL) but not by fatty acid amide hydrolase. Anatomic and optogenetic circuit mapping indicated that MSDB GABA neurons monosynaptically project to cholinergic neurons of the ventral MHb. To test the behavioral significance of this MSDB–MHb endocannabinoid signaling, we induced MSDB-specific knockout of CB1 or MAGL via injection of virally-delivered Cre recombinase into the MSDB of Cnr1loxP/loxP or MgllloxP/loxP mice. Relative to control mice, MSDB-specific knockout of CB1 or MAGL bidirectionally modulated 2-AG signaling in the ventral MHb and led to opposing effects on anxiety- and depressive-like behavior. Thus, depression of synaptic GABA release in the MSDB-ventral MHb pathway may represent a potential mechanism whereby endocannabinoids exert anxiolytic and antidepressant-like effects.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Data availability
All data necessary for evaluation of the paper’s conclusions are present in the main text and/or Supplementary Information.
References
Walsh Z, Gonzalez R, Crosby K, M ST, Carroll C, Bonn-Miller MO. Medical cannabis and mental health: a guided systematic review. Clin Psychol Rev. 2017;51:15–29.
Sexton M, Cuttler C, Finnell JS, Mischley LK. A cross-sectional survey of medical cannabis users: patterns of use and perceived efficacy. Cannabis Cannabinoid Res. 2016;1:131–8.
Blankman JL, Simon GM, Cravatt BF. A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem Biol. 2007;14:1347–56.
Zhong P, Wang W, Pan B, Liu X, Zhang Z, Long JZ, et al. Monoacylglycerol lipase inhibition blocks chronic stress-induced depressive-like behaviors via activation of mTOR signaling. Neuropsychopharmacol Off Publ Am Coll Neuropsychopharmacol. 2014;39:1763–76.
Zhang Z, Wang W, Zhong P, Liu SJ, Long JZ, Zhao L, et al. Blockade of 2-arachidonoylglycerol hydrolysis produces antidepressant-like effects and enhances adult hippocampal neurogenesis and synaptic plasticity. Hippocampus. 2015;25:16–26.
Bedse G, Bluett RJ, Patrick TA, Romness NK, Gaulden AD, Kingsley PJ, et al. Therapeutic endocannabinoid augmentation for mood and anxiety disorders: comparative profiling of FAAH, MAGL and dual inhibitors. Transl Psychiatry. 2018;8:92.
Bedse G, Hartley ND, Neale E, Gaulden AD, Patrick TA, Kingsley PJ, et al. Functional redundancy between canonical endocannabinoid signaling systems in the modulation of anxiety. Biol Psychiatry. 2017;82:488–99.
Jenniches I, Ternes S, Albayram O, Otte DM, Bach K, Bindila L, et al. Anxiety, stress, and fear response in mice with reduced endocannabinoid levels. Biol Psychiatry. 2016;79:858–68.
Shonesy BC, Bluett RJ, Ramikie TS, Baldi R, Hermanson DJ, Kingsley PJ, et al. Genetic disruption of 2-arachidonoylglycerol synthesis reveals a key role for endocannabinoid signaling in anxiety modulation. Cell Rep. 2014;9:1644–53.
Cravatt BF, Giang DK, Mayfield SP, Boger DL, Lerner RA, Gilula NB. Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature. 1996;384:83–7.
Marcus DJ, Bedse G, Gaulden AD, Ryan JD, Kondev V, Winters ND, et al. Endocannabinoid signaling collapse mediates stress-induced amygdalo-cortical strengthening. Neuron. 2020;105:1062–76.e1066.
Shen CJ, Zheng D, Li KX, Yang JM, Pan HQ, Yu XD, et al. Cannabinoid CB1 receptors in the amygdalar cholecystokinin glutamatergic afferents to nucleus accumbens modulate depressive-like behavior. Nat Med. 2019;25:337–49.
Hsu YW, Wang SD, Wang S, Morton G, Zariwala HA, de la Iglesia HO, et al. Role of the dorsal medial habenula in the regulation of voluntary activity, motor function, hedonic state, and primary reinforcement. J Neurosci Off J Soc Neurosci. 2014;34:11366–84.
Xu C, Sun Y, Cai X, You T, Zhao H, Li Y, et al. Medial habenula-interpeduncular nucleus circuit contributes to anhedonia-like behavior in a rat model of depression. Front Behav Neurosci. 2018;12:238.
Molas S, DeGroot SR, Zhao-Shea R, Tapper AR. Anxiety and nicotine dependence: emerging role of the habenulo-interpeduncular axis. Trends Pharmacol Sci. 2017;38:169–80.
Yamaguchi T, Danjo T, Pastan I, Hikida T, Nakanishi S. Distinct roles of segregated transmission of the septo-habenular pathway in anxiety and fear. Neuron. 2013;78:537–44.
Zhao-Shea R, DeGroot SR, Liu L, Vallaster M, Pang X, Su Q, et al. Increased CRF signalling in a ventral tegmental area-interpeduncular nucleus-medial habenula circuit induces anxiety during nicotine withdrawal. Nat Commun. 2015;6:6770.
Ranft K, Dobrowolny H, Krell D, Bielau H, Bogerts B, Bernstein HG. Evidence for structural abnormalities of the human habenular complex in affective disorders but not in schizophrenia. Psychol Med. 2010;40:557–67.
Suarez J, Ortiz O, Puente N, Bermudez-Silva FJ, Blanco E, Fernandez-Llebrez P, et al. Distribution of diacylglycerol lipase alpha, an endocannabinoid synthesizing enzyme, in the rat forebrain. Neuroscience. 2011;192:112–31.
Kano M, Ohno-Shosaku T, Hashimotodani Y, Uchigashima M, Watanabe M. Endocannabinoid-mediated control of synaptic transmission. Physiological Rev. 2009;89:309–80.
Castillo PE, Younts TJ, Chavez AE, Hashimotodani Y. Endocannabinoid signaling and synaptic function. Neuron. 2012;76:70–81.
Qin C, Luo M. Neurochemical phenotypes of the afferent and efferent projections of the mouse medial habenula. Neuroscience. 2009;161:827–37.
Gray JA, McNaughton N. The neuropsychology of anxiety: an enquiry into the functions of the septo-hippocampal system. 2nd ed. Oxford, New York: Oxford University Press; 2000, xvi, p. 424.
Pratt JA. The neuroanatomical basis of anxiety. Pharmacol Ther. 1992;55:149–81.
Wang F, Flanagan J, Su N, Wang LC, Bui S, Nielson A, et al. RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn. 2012;14:22–9.
Okamoto Y, Morishita J, Tsuboi K, Tonai T, Ueda N. Molecular characterization of a phospholipase D generating anandamide and its congeners. J Biol Chem. 2004;279:5298–305.
Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010;13:133–40.
Kim U, Chung LY. Dual GABAergic synaptic response of fast excitation and slow inhibition in the medial habenula of rat epithalamus. J Neurophysiol. 2007;98:1323–32.
Choi K, Lee Y, Lee C, Hong S, Lee S, Kang SJ, et al. Optogenetic activation of septal GABAergic afferents entrains neuronal firing in the medial habenula. Sci Rep. 2016;6:34800.
Gorlich A, Antolin-Fontes B, Ables JL, Frahm S, Slimak MA, Dougherty JD, et al. Reexposure to nicotine during withdrawal increases the pacemaking activity of cholinergic habenular neurons. Proc Natl Acad Sci USA. 2013;110:17077–82.
Reiner A, Veenman CL, Medina L, Jiao Y, Del Mar N, Honig MG. Pathway tracing using biotinylated dextran amines. J Neurosci Methods. 2000;103:23–37.
McMahon HT, Bolshakov VY, Janz R, Hammer RE, Siegelbaum SA, Südhof TC. Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release. Proc Natl Acad Sci USA. 1996;93:4760–4.
Pizzarelli R, Griguoli M, Zacchi P, Petrini EM, Barberis A, Cattaneo A, et al. Tuning GABAergic inhibition: gephyrin molecular organization and functions. Neuroscience. 2020;439:125–36.
Dehmelt L, Halpain S. The MAP2/Tau family of microtubule-associated proteins. Genome Biol. 2004;6:204.
Sotty F, Danik M, Manseau F, Laplante F, Quirion R, Williams S. Distinct electrophysiological properties of glutamatergic, cholinergic and GABAergic rat septohippocampal neurons: novel implications for hippocampal rhythmicity. J Physiol. 2003;551:927–43.
Gulyas AI, Cravatt BF, Bracey MH, Dinh TP, Piomelli D, Boscia F, et al. Segregation of two endocannabinoid-hydrolyzing enzymes into pre- and postsynaptic compartments in the rat hippocampus, cerebellum and amygdala. Eur J Neurosci. 2004;20:441–58.
Yu T, Guo M, Garza J, Rendon S, Sun XL, Zhang W, et al. Cognitive and neural correlates of depression-like behaviour in socially defeated mice: an animal model of depression with cognitive dysfunction. Int J Neuropsychopharmacol. 2011;14:303–17.
Patel S, Hillard CJ. Role of endocannabinoid signaling in anxiety and depression. Curr Top Behav Neurosci. 2009;1:347–71.
Bourin M, Hascoet M. The mouse light/dark box test. Eur J Pharmacol. 2003;463:55–65.
Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol. 2003;463:3–33.
Hogg S. A review of the validity and variability of the elevated plus-maze as an animal model of anxiety. Pharmacol Biochem Behav. 1996;54:21–30.
de Brouwer G, Fick A, Harvey BH, Wolmarans W. A critical inquiry into marble-burying as a preclinical screening paradigm of relevance for anxiety and obsessive-compulsive disorder: mapping the way forward. Cogn Affect Behav Neurosci. 2019;19:1–39.
Bodnoff SR, Suranyi-Cadotte B, Quirion R, Meaney MJ. A comparison of the effects of diazepam versus several typical and atypical anti-depressant drugs in an animal model of anxiety. Psychopharmacology. 1989;97:277–9.
Rex A, Voigt JP, Voits M, Fink H. Pharmacological evaluation of a modified open-field test sensitive to anxiolytic drugs. Pharmacol Biochem Behav. 1998;59:677–83.
Liu MY, Yin CY, Zhu LJ, Zhu XH, Xu C, Luo CX, et al. Sucrose preference test for measurement of stress-induced anhedonia in mice. Nat Protoc. 2018;13:1686–98.
Rizvi SJ, Pizzagalli DA, Sproule BA, Kennedy SH. Assessing anhedonia in depression: potentials and pitfalls. Neurosci Biobehav Rev. 2016;65:21–35.
Castagne V, Moser P, Roux S, Porsolt RD. Rodent models of depression: forced swim and tail suspension behavioral despair tests in rats and mice. Curr Protoc Neurosci. 2011;Chapter 8:Unit 8.10A.
Zhang GW, Shen L, Zhong W, Xiong Y, Zhang LI, Tao HW. Transforming sensory cues into aversive emotion via septal-habenular pathway. Neuron. 2018;99:1016–28.e1015.
Koppensteiner P, Galvin C, Ninan I. Development- and experience-dependent plasticity in the dorsomedial habenula. Mol Cell Neurosci. 2016;77:105–12.
Maccarrone M. Metabolism of the endocannabinoid anandamide: open questions after 25 years. Front Mol Neurosci. 2017;10:166.
Soria-Gomez E, Busquets-Garcia A, Hu F, Mehidi A, Cannich A, Roux L, et al. Habenular CB1 receptors control the expression of aversive memories. Neuron. 2015;88:306–13.
Jacob W, Yassouridis A, Marsicano G, Monory K, Lutz B, Wotjak CT. Endocannabinoids render exploratory behaviour largely independent of the test aversiveness: role of glutamatergic transmission. Genes Brain Behav. 2009;8:685–98.
Bluett RJ, Baldi R, Haymer A, Gaulden AD, Hartley ND, Parrish WP, et al. Endocannabinoid signalling modulates susceptibility to traumatic stress exposure. Nat Commun. 2017;8:14782.
Gamble-George JC, Conger JR, Hartley ND, Gupta P, Sumislawski JJ, Patel S. Dissociable effects of CB1 receptor blockade on anxiety-like and consummatory behaviors in the novelty-induced hypophagia test in mice. Psychopharmacology. 2013;228:401–9.
Chen Y, Liu X, Vickstrom CR, Liu MJ, Zhao L, Viader A, et al. Neuronal and astrocytic monoacylglycerol lipase limit the spread of endocannabinoid signaling in the cerebellum. eNeuro. 2016;3:e0048–16.
Liu X, Chen Y, Vickstrom CR, Li Y, Viader A, Cravatt BF, et al. Coordinated regulation of endocannabinoid-mediated retrograde synaptic suppression in the cerebellum by neuronal and astrocytic monoacylglycerol lipase. Sci Rep. 2016;6:35829.
Vandecasteele M, Varga V, Berenyi A, Papp E, Bartho P, Venance L, et al. Optogenetic activation of septal cholinergic neurons suppresses sharp wave ripples and enhances theta oscillations in the hippocampus. Proc Natl Acad Sci USA. 2014;111:13535–40.
Justus D, Dalugge D, Bothe S, Fuhrmann F, Hannes C, Kaneko H, et al. Glutamatergic synaptic integration of locomotion speed via septoentorhinal projections. Nat Neurosci. 2017;20:16–9.
Freund TF, Antal M. GABA-containing neurons in the septum control inhibitory interneurons in the hippocampus. Nature. 1988;336:170–3.
Hoffman AF, Riegel AC, Lupica CR. Functional localization of cannabinoid receptors and endogenous cannabinoid production in distinct neuron populations of the hippocampus. Eur J Neurosci. 2003;18:524–34.
Le Foll B, French L. Transcriptomic characterization of the human habenula highlights drug metabolism and the neuroimmune system. Front Neurosci. 2018;12:742.
Han S, Yang SH, Kim JY, Mo S, Yang E, Song KM, et al. Down-regulation of cholinergic signaling in the habenula induces anhedonia-like behavior. Sci Rep. 2017;7:900.
Ma Z, Zhong Y, Hines CS, Wu Y, Li Y, Pang M, et al. Identifying generalized anxiety disorder using resting state habenular circuitry. Brain Imaging Behav. 2019. https://doi.org/10.1007/s11682-019-00055-1.
Darmani NA, Janoyan JJ, Kumar N, Crim JL. Behaviorally active doses of the CB1 receptor antagonist SR 141716A increase brain serotonin and dopamine levels and turnover. Pharmacol Biochem Behav. 2003;75:777–87.
Kaifosh P, Lovett-Barron M, Turi GF, Reardon TR, Losonczy A. Septo-hippocampal GABAergic signaling across multiple modalities in awake mice. Nat Neurosci. 2013;16:1182–4.
Acsady L, Arabadzisz D, Katona I, Freund TF. Topographic distribution of dorsal and median raphe neurons with hippocampal, septal and dual projection. Acta Biol Hung. 1996;47:9–19.
Han X, He Y, Bi GH, Zhang HY, Song R, Liu QR, et al. CB1 receptor activation on VgluT2-expressing glutamatergic neurons underlies delta(9)-tetrahydrocannabinol (Delta(9)-THC)-induced aversive effects in mice. Sci Rep. 2017;7:12315.
Viader A, Blankman JL, Zhong P, Liu X, Schlosburg JE, Joslyn CM, et al. Metabolic interplay between astrocytes and neurons regulates endocannabinoid action. Cell Rep. 2015;12:798–808.
Zhong P, Vickstrom CR, Liu X, Hu Y, Yu L, Yu HG, et al. HCN2 channels in the ventral tegmental area regulate behavioral responses to chronic stress. eLife. 2018;7:e32420.
Vickstrom CR, Liu X, Zhang Y, Mu L, Kelly TJ, Yan X, et al. T-type calcium channels contribute to burst firing in a subpopulation of medial habenula neurons. eNeuro. 2020;7.
Ting JT, Lee BR, Chong P, Soler-Llavina G, Cobbs C, Koch C, et al. Preparation of acute brain slices using an optimized N-Methyl-D-glucamine protective recovery method. J Vis Exp. 2018;132:e53825.
Cho CH, Lee S, Kim A, Yarishkin O, Ryoo K, Lee YS, et al. TMEM16A expression in cholinergic neurons of the medial habenula mediates anxiety-related behaviors. EMBO Rep. 2020;21:e48097.
Ge F, Mu P, Guo R, Cai L, Liu Z, Dong Y, et al. Chronic sleep fragmentation enhances habenula cholinergic neural activity. Mol Psychiatry. 2019.
Acknowledgements
This work was supported by National Institutes of Health Grants MH115536 (to CRV), MH121454 (to QSL and CJH), DA047269 (to QSL), and DA035217 (to QSL). It was also partially funded through the Research and Education Initiative Fund, a component of the Advancing a Healthier Wisconsin endowment at the Medical College of Wisconsin. CRV is a member of the Medical Scientist Training Program at MCW, which is partially supported by a training grant from NIGMS T32-GM080202.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
About this article
Cite this article
Vickstrom, C.R., Liu, X., Liu, S. et al. Role of endocannabinoid signaling in a septohabenular pathway in the regulation of anxiety- and depressive-like behavior. Mol Psychiatry 26, 3178–3191 (2021). https://doi.org/10.1038/s41380-020-00905-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41380-020-00905-1
This article is cited by
-
Psilocybin analog 4-OH-DiPT enhances fear extinction and GABAergic inhibition of principal neurons in the basolateral amygdala
Neuropsychopharmacology (2024)
-
Neuropeptide Y in the medial habenula alleviates migraine-like behaviors through the Y1 receptor
The Journal of Headache and Pain (2023)
-
cAMP-mediated upregulation of HCN channels in VTA dopamine neurons promotes cocaine reinforcement
Molecular Psychiatry (2023)
-
Integrative Analysis of Morphine-Induced Differential Circular RNAs and ceRNA Networks in the Medial Prefrontal Cortex
Molecular Neurobiology (2023)
-
Basal Forebrain Cholinergic Innervation Induces Depression-Like Behaviors Through Ventral Subiculum Hyperactivation
Neuroscience Bulletin (2023)