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CtBPs promote mitotic fidelity through their activities in the cell nucleus

Oncogene volume 30, pages 1272–1280 (2011)Cite this article

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Abstract

CtBPs form NADH-sensitive chromatin-modifying complexes, which link cellular metabolism to gene transcription. They also function in the cytoplasm to regulate Golgi fissioning; their inhibition can consequently cause a Golgi-dependent checkpoint in G2. We have recently identified a novel role of CtBPs in the maintenance of mitotic fidelity; inhibition of CtBP synthesis resulting in reduced association of aurora B with mitotic chromatin and aberrant segregation of chromosomes. Here, we demonstrate that it is the interaction of CtBPs with transcriptional regulators and/or chromatin-modifying enzymes in the cell nucleus, rather than their role in Golgi fission, which is critical for the maintenance of mitotic fidelity.

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Acknowledgements

We are grateful to Matthew Darley for the generation of plasmid reagents, Patrick Duriez for the preparation of GST fusion proteins for microinjection, and the Southampton School of Medicine Bioimaging Unit for assistance with live cell imaging. Funding for the work was from the UK Breast Cancer Campaign.

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Authors and Affiliations

  1. Southampton Cancer Research UK Centre, University of Southampton School of Medicine, Southampton, Hampshire, UK

    C N Birts & J P Blaydes

  2. Medicines Toxicology Evaluation Section, Office of Prescription Medicines, Therapeutic Goods Administration, Woden, Australian Capital Territory, Australia

    L M Bergman

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  1. C N Birts
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Correspondence to J P Blaydes.

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Birts, C., Bergman, L. & Blaydes, J. CtBPs promote mitotic fidelity through their activities in the cell nucleus. Oncogene 30, 1272–1280 (2011). https://doi.org/10.1038/onc.2010.507

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