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Deacetylation of cortactin by SIRT1 promotes cell migration

Oncogene volume 28, pages 445–460 (2009)Cite this article

Abstract

Cortactin binds F-actin and promotes cell migration. We showed earlier that cortactin is acetylated. Here, we identify SIRT1 (a class III histone deacetylase) as a cortactin deacetylase and p300 as a cortactin acetylase. We show that SIRT1 deacetylates cortactin in vivo and in vitro and that the SIRT1 inhibitor EX-527 increases amounts of acetylated cortactin in ovarian cancer cells. We also show that p300 acetylates cortactin in vivo and that cells lacking or depleted of p300 express less-acetylated cortactin than do control cells. Deletion analysis mapped the SIRT1-binding domain of cortactin to its repeat region, which also binds F-actin. Mouse embryo fibroblasts (MEFs) lacking sir2α (the mouse homolog of SIRT1) migrated more slowly than did wild-type cells. The expression of SIRT1 in sir2α-null cells restored migratory capacity, as did expression of a deacetylation-mimetic mutant of cortactin. SIRT1 and cortactin were more abundant in breast tumor tissue than in their normal counterparts, whereas SIRT1 expression inversely correlates with the ratio of acetylation cortactin versus total cortactin. These data suggest that deacetylation of cortactin is associated with high levels of SIRT1 and tumorigenesis. Finally, breast and ovarian cancer cell lines expressing an acetylation mimetic mutant of cortactin are less motile than that of control cells, whereas cells expressing the deacetylation mimetic mutant of cortactin migrate faster than that of control cells in Transwell migration assays. In summary, our results suggest that cortactin is a novel substrate for SIRT1 and p300 and, for the first time, a possible role for SIRT1 in cell motility through deacetylation of cortactin.

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Acknowledgements

We are indebted to Dr Edward Seto for sirtuins plasmids. We thank Dr Michael W McBurney for sir2α+/+ and sir2α−/− MEFs; Dr James A DeCaprio for p300+/+ and p300−/− MEFs; Dr Don F Cameron for microscope usage; Drs Srikumar Chellappan, Gloria Ferreira, Mike Yuan, Zheng Shen, Mei Sun and Hua Yang for helpful discussions; Ms Kathleen Merkler for critical reading of this manuscript and Dr Ulmesh Jinwal for technical assistance. We also thank the H Lee Moffitt Cancer Center Analytic Microscopy Core Facility for their technical support and H Lee Moffitt Cancer Center Tissue Procurement for breast and ovarian tissues. This work was supported partly by ‘Start-up’ funds from the USF College of Medicine and H Lee Moffitt Cancer Center, an USF New Researcher grant, a Marsha Rivkin Center Scientific Scholar award for ovarian cancer research and a Bankhead-Coley Biomedical Research Program New Investigator Research Grant to XZ.

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  1. Department of Pathology and Cell Biology, College of Medicine, University of South Florida, Tampa, FL, USA

    Y Zhang, M Zhang, H Dong, S Yong, X Li, P A Kruk, W Bai, S V Nicosia & X Zhang

  2. Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA

    N Olashaw, P A Kruk, J Q Cheng, W Bai, J Chen & X Zhang

  3. Experimental Therapeutics Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA

    S V Nicosia

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Zhang, Y., Zhang, M., Dong, H. et al. Deacetylation of cortactin by SIRT1 promotes cell migration. Oncogene 28, 445–460 (2009). https://doi.org/10.1038/onc.2008.388

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