Abstract
Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.
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References
Isken, O. & Maquat, L.E. Genes Dev. 21, 1833–1856 (2007).
Behm-Ansmant, I. et al. FEBS Lett. 581, 2845–2853 (2007).
Amrani, N., Sachs, M.S. & Jacobson, A. Nat. Rev. Mol. Cell Biol. 7, 415–425 (2006).
Baker, K.E. & Parker, R. Curr. Opin. Cell Biol. 16, 293–299 (2004).
Muhlrad, D. & Parker, R. Nature 370, 578–581 (1994).
Lejeune, F., Li, X. & Maquat, L.E. Mol. Cell 12, 675–687 (2003).
Eberle, A.B., Lykke-Andersen, S., Muhlemann, O. & Jensen, T.H. Nat. Struct. Mol. Biol. 16, 49–55 (2009).
Atkin, A.L. et al. J. Biol. Chem. 272, 22163–22172 (1997).
Mangus, D.A. & Jacobson, A. Methods 17, 28–37 (1999).
Sheth, U. & Parker, R. Cell 125, 1095–1109 (2006).
Isken, O. et al. Cell 133, 314–327 (2008).
Muhlrad, D. & Parker, R. Mol. Biol. Cell 10, 3971–3978 (1999).
Hu, W., Sweet, T.J., Chamnongpol, S., Baker, K.E. & Coller, J. Nature 461, 225–229 (2009).
Stalder, L. & Muhlemann, O. RNA 15, 1265–1273 (2009).
Kshirsagar, M. & Parker, R. Genetics 166, 729–739 (2004).
Decker, C.J., Teixeira, D. & Parker, R. J. Cell Biol. 179, 437–449 (2007).
Rehwinkel, J., Behm-Ansmant, I., Gatfield, D. & Izaurralde, E. RNA 11, 1640–1647 (2005).
Coller, J. & Parker, R. Cell 122, 875–886 (2005).
Inada, T. et al. RNA 8, 948–958 (2002).
Wang, Z., Jiao, X., Carr-Schmid, A. & Kiledjian, M. Proc. Natl. Acad. Sci. USA 99, 12663–12668 (2002).
Acknowledgements
The authors thank members of the Baker and Coller laboratories for critical evaluation of the manuscript. W.H. is supported by the American Heart Association. C.P. is supported by an undergraduate stipend provided by the US National Science Foundation. Funding was provided by the US National Institutes of Health (GM080465).
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W.H., J.C. and K.E.B. designed experimental strategies; W.H. and C.P. performed experimental analysis and interpreted data; J.C. and K.E.B. interpreted data and wrote the manuscript.
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Hu, W., Petzold, C., Coller, J. et al. Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae. Nat Struct Mol Biol 17, 244–247 (2010). https://doi.org/10.1038/nsmb.1734
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DOI: https://doi.org/10.1038/nsmb.1734
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