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Leadzyme RNA catalysis

Nature Structural Biology volume 6, pages 208–209 (1999)Cite this article

Abstract

The structure of a lead-dependent ribozyme reveals binding sites of divalent ions. One of them mimics the binding of catalytic lead and allows modeling of the cleavage reaction.

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Figure 1: a, The sequence numbering with the cleavage site indicated by •.
Figure 2: The pairs forming two small three base pair parallel helices between the independent molecules of leadzyme in the crystal7.
Figure 3: Some differences between magnesium and lead ions.

References

  1. Pley H.W., Flaherty, K.M. & McKay, D.B. Nature 372, 68– 74 (1994).

    Article  CAS  Google Scholar 

  2. Scott W.G., Finch J.T. & Klug, A. Cell 81, 991– 1002 (1995).

    Article  CAS  Google Scholar 

  3. Murray, J.B. et al. Cell 92, 665–673 (1998).

    Article  CAS  Google Scholar 

  4. Cate, J.H. et al. Science 273, 1678–1685 (1996).

    Article  CAS  Google Scholar 

  5. Golden, B.L., Gooding, E.R., Podell, E.R. & Cech, T.R. Science 282, 259–274 ( 1999).

    Article  Google Scholar 

  6. Ferré-D'Amaré, A.R., Zhou, K. & Doudna, J.A. Nature 395, 567–574 (1999).

    Article  Google Scholar 

  7. Wedekind, J. E. & McKay, D.B. Nature Struct. Biol. 6, 261–268 ( 1999).

    Article  CAS  Google Scholar 

  8. Werner, C., Krebs, B., Keith, G. & Dirheimer, G. Biochim. Biophys. Acta 432, 161–175 ( 1976).

    Article  CAS  Google Scholar 

  9. Brown, R.S., Dewan, J.C. & Klug, A. Biochemistry 24, 4785– 4801 (1985).

    Article  CAS  Google Scholar 

  10. Pan, T. & Uhlenbeck, O.C. Nature 358, 560–563 (1992).

    Article  CAS  Google Scholar 

  11. Hoogstraten, C.G., Legault, P. & Pardi, A. J. Mol. Biol. 284, 337– 350 (1998).

    Article  CAS  Google Scholar 

  12. Westhof, E., Rao, S.T. & Sundaralingam, M. J. Mol. Biol. 142, 331– 361 (1980).

    Article  CAS  Google Scholar 

  13. Cruse, W.B.T. et al. Biochemistry 22, 1833– 1839 (1983).

    Article  CAS  Google Scholar 

  14. Chartrand, P., Usman, N. & Cedergren, R. Biochemistry 36, 3145– 3150 (1997).

    Article  CAS  Google Scholar 

  15. Ohmichi, T. & Sugimoto, N. Biochemistry 36, 3514–3521 (1997).

    Article  CAS  Google Scholar 

  16. Jason, P.R. et al. Biophys. J. 76, 65– 75 (1999).

    Article  Google Scholar 

  17. Rippe, K., Fritsch, V., Westhof, E. & Jovin, T.M. EMBO J. 11, 3777–3786 (1992).

    Article  CAS  Google Scholar 

  18. Harrison, P. G. Coord. Chem. Rev. 20, 1–36 (1976).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. the Institut de Biologie Moléculaire et Cellulaire, CNRS, 5 rue René Descartes, Strasbourg-Cedex, F-67084, France

    Eric Westhof & Thomas Hermann

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  1. Eric Westhof
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  2. Thomas Hermann
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Westhof, E., Hermann, T. Leadzyme RNA catalysis. Nat Struct Mol Biol 6, 208–209 (1999). https://doi.org/10.1038/6643

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