Successful application of the CRISPR–Cas method for genome editing has been reported in cynomolgus monkeys (Macaca fascicularis). The researchers injected the components of the system, Cas9 mRNA and mixtures of 5 single-chain guide RNAs into 186 zygotes, 83 of which were transferred into 29 surrogate females. A total of ten pregnancies were established. So far, a female twin pair harbouring Cas9–RNA-mediated site-specific modifications in the simultaneously targeted genes Pparg and Rag1 has been born. Of note, no off-target mutations were detected in either animal.
References
Niu, Y. et al. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell 156, 836–843 (2014)
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Koch, L. High-precision gene editing in monkeys is feasible. Nat Rev Genet 15, 218 (2014). https://doi.org/10.1038/nrg3712
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DOI: https://doi.org/10.1038/nrg3712
