Many small-molecule drugs target DNA-associated processes such as transcription, modification and replication. This paper devised a method — called Chem-seq — based on ligand-affinity capture and massively parallel DNA sequencing to identify the genome binding sites of a bromodomain inhibitor (JQ1), a cyclin-dependent kinase 9 inhibitor (AT7519) and the DNA intercalator psoralen in multiple myeloma cells. The authors note that this method could be used to help understand the action and specificity of many small molecules.
References
Anders, L. et al. Genome-wide localization of small molecules. Nature Biotech. 32, 92–96 (2014)
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Harrison, C. Genome-wide target identification. Nat Rev Drug Discov 13, 104 (2014). https://doi.org/10.1038/nrd4248
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DOI: https://doi.org/10.1038/nrd4248
