Comment

CRISPR–Cas in the laboratory classroom

CRISPR–Cas is an adaptive immune system found in Bacteria and Archaea that confers sequence-specific protection against invasion by foreign nucleic acids. CRISPR–Cas is also a powerful tool for microbiology education and has been an inspiring model for our students for the past three years.

  • Subscribe to Nature Microbiology for full access:

    $59

    Subscribe

  • Purchase article full text and PDF:

    $18

    Buy now

Additional access options:

Already a subscriber?  Log in  now or  Register  for online access.

References

  1. 1.

    Nature 531, 160–163 (2016).

  2. 2.

    et al. Science 337, 816–821 (2012).

  3. 3.

    et al. Science 339, 819–23 (2013).

  4. 4.

    et al. Science 315, 1709–1712 (2007).

  5. 5.

    et al. Mol. Microbiol. 43, 1565–1575 (2002).

  6. 6.

    et al. Nat. Rev. Microbiol. 13, 722–736 (2015).

  7. 7.

    , & Nat. Commun. 5, 4399 (2014).

  8. 8.

    et al. Science 321, 960–964 (2008).

  9. 9.

    et al. Nature 471, 602–607 (2011).

  10. 10.

    et al. Nature 468, 67–71 (2010).

  11. 11.

    et al. Nat. Protoc. 12, 547–565 (2017).

  12. 12.

    Int. J. Food Microbiol. 126, 274–277 (2008).

  13. 13.

    et al. J. Bacteriol. 190, 1390–1400 (2008).

  14. 14.

    et al. Biochem. Mol. Biol. Educ. 44, 263–275 (2016).

  15. 15.

    & Biochem. Mol. Biol. Educ. (2016).

Download references

Acknowledgements

We are grateful to S. Charette, B. Conway, A. Culley, A. Darveau, C. Duchaine, A. Hynes, M.-L. Lemay and L. Saucier for critical reading. We also thank M. Dion and A. Renaud for their comments on their experience attending the course. S.M. acknowledges funding from the Natural Sciences and Engineering Research Council of Canada (Discovery program). S.M. holds a T1 Canada Research Chair in Bacteriophages.

Author information

Affiliations

  1. Luc Trudel, Michel Frenette and Sylvain Moineau are at Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, 1045, avenue de la Médecine, Québec, PQ G1V 0A6, Canada.

    • Luc Trudel
    • , Michel Frenette
    •  & Sylvain Moineau
  2. M.F. and S.M. are also at Groupe de recherche en écologie buccale, Faculté de médecine dentaire, 2420 rue de la Terrasse, Université Laval, Québec, PQ G1V 0A6, Canada.

    • Michel Frenette
    •  & Sylvain Moineau
  3. S.M. is also at Félix d'Hérelle Reference Center for Bacterial Viruses, Faculté de médecine dentaire, 2420 rue de la Terrasse, Université Laval, Québec, PQ G1V 0A6, Canada.

    • Sylvain Moineau

Authors

  1. Search for Luc Trudel in:

  2. Search for Michel Frenette in:

  3. Search for Sylvain Moineau in:

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Sylvain Moineau.