CRISPR-Cas systems

Definition

CRISPR-Cas systems are prokaryotic immune systems that confer resistance to foreign genetic elements such as plasmids and phages. CRISPR-Cas systems have been exploited for targeted genome editing.

Latest Research and Reviews

  • Research | | open

    Zbtb16-encoded transcription factor PLZF directs the differentiation of multiple innate and innate-like cell lineages, but how Zbtb16 itself is regulated remains unclear. Here the authors show, using CRISPR gene editing, ATAC-seq and ChIP-seq, that specific Runx1-bound enhancer elements critically modulate lineage-dependent expressions of PLZF.

    • Ai-Ping Mao
    • , Isabel E. Ishizuka
    • , Darshan N. Kasal
    • , Malay Mandal
    •  & Albert Bendelac
  • Research | | open

    Genetic isolation of a genetically modified organism represents a useful strategy for biocontainment. Here the authors use dCas9-VP64-driven gene expression to construct a ‘species-like’ barrier to reproduction between two otherwise compatible populations.

    • Maciej Maselko
    • , Stephen C. Heinsch
    • , Jeremy M. Chacón
    • , William R. Harcombe
    •  & Michael J. Smanski
  • Research |

    Genome editing in human zygotes shows that OCT4 is required for normal development at an earlier stage in humans than in mice.

    • Norah M. E. Fogarty
    • , Afshan McCarthy
    • , Kirsten E. Snijders
    • , Benjamin E. Powell
    • , Nada Kubikova
    • , Paul Blakeley
    • , Rebecca Lea
    • , Kay Elder
    • , Sissy E. Wamaitha
    • , Daesik Kim
    • , Valdone Maciulyte
    • , Jens Kleinjung
    • , Jin-Soo Kim
    • , Dagan Wells
    • , Ludovic Vallier
    • , Alessandro Bertero
    • , James M. A. Turner
    •  & Kathy K. Niakan
    Nature 550, 67–73
  • Research | | open

    CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.

    • Xiaoyu Chen
    • , Josephine M. Janssen
    • , Jin Liu
    • , Ignazio Maggio
    • , Anke E. J. ‘t Jong
    • , Harald M.M. Mikkers
    •  & Manuel A. F. V. Gonçalves
  • Research | | open

    Understanding the link between epigenetic marks and gene regulation requires the development of new tools to directly manipulate chromatin. Here the authors demonstrate a Cas9-based system to recruit chromatin remodelers to loci of interest, allowing rapid, reversible manipulation of epigenetic states.

    • Simon M. G. Braun
    • , Jacob G. Kirkland
    • , Emma J. Chory
    • , Dylan Husmann
    • , Joseph P. Calarco
    •  & Gerald R. Crabtree
  • Research | | open

    β-thalassemia is characterised by the presence of an excess of α-globin chains, which contribute to erythrocyte pathology. Here the authors use CRISP/Cas9 to reduce α-globin expression in hematopoietic precursors, and show effectiveness in xenograft assays in mice.

    • Sachith Mettananda
    • , Chris A. Fisher
    • , Deborah Hay
    • , Mohsin Badat
    • , Lynn Quek
    • , Kevin Clark
    • , Philip Hublitz
    • , Damien Downes
    • , Jon Kerry
    • , Matthew Gosden
    • , Jelena Telenius
    • , Jackie A. Sloane-Stanley
    • , Paula Faustino
    • , Andreia Coelho
    • , Jessica Doondeea
    • , Batchimeg Usukhbayar
    • , Paul Sopp
    • , Jacqueline A. Sharpe
    • , Jim R. Hughes
    • , Paresh Vyas
    • , Richard J. Gibbons
    •  & Douglas R. Higgs

News and Comment