The best way to capture exons

Clark, M. J. et al. Nat. Biotechnol. 29, 908–914 (2011).

Sequencing only the exons in a genome narrows the search for functional variation. Clark et al. compared tools that capture the exome by hybridizing mRNA to oligonucleotide baits in solution. Nimblegen baits covered less but overlapped at high density, giving excellent sensitivity for the least sequencing. Illumina and Agilent baits covered more bases at low density, facilitating the discovery of more variants upon deeper sequencing.