Abstract
The initiation of a primary immune response requires contact between dendritic cells (DCs) and resting T cells. However, little is known about the proteins that mediate this initial contact. We show here that neuropilin-1, a receptor involved in axon guidance, was expressed by human DCs and resting T cells both in vitro and in vivo. The initial contact between DCs and resting T cells led to neuropilin-1 polarization on T cells. DCs and resting T cells specifically bound soluble neuropilin-1, and resting T cells formed clusters with neuropilin-1–transfected COS-7 cells in a neuropilin-1–dependent manner. Functionally, preincubation of DCs or resting T cells with blocking neuropilin-1 antibodies inhibited DC-induced proliferation of resting T cells. These data suggest that neuropilin-1 mediates interactions between DCs and T cells that are essential for initiation of the primary immune response and show parallels between the nervous and immune systems.
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Acknowledgements
We thank M. Tessier-Lavigne and Z. He for the gift of the NP-1 construct and of anti–neuropilin-1 (intracytoplasmic and blocking anti–neuropilin-1); A. Amara for the gift of anti-DC-SIGN; A. Trautmann, G. Bismuth and A. Chédotal for their advice; and A. Baruchel for his advice and encouragement. Supported by the INSERM, the CNRS and the Association pour la Recherche sur le Cancer(grant number 5715). M. C. was supported by the Fondation pour la Recherche Médicale.
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Tordjman, R., Lepelletier, Y., Lemarchandel, V. et al. A neuronal receptor, neuropilin-1, is essential for the initiation of the primary immune response. Nat Immunol 3, 477–482 (2002). https://doi.org/10.1038/ni789
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DOI: https://doi.org/10.1038/ni789
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